Tetramethylrhodamine-5-iodoacetamide
Based on 1 Customer Validation
Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) is a thiol-selective reactive dye that is used to non-specifically label proteins via the cysteine residues. Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) can be used to covalently label DNA fragments.
For research use only. We do not sell to patients.
- Purity: 86.61%
- CAS No.: 114458-99-0
- Formula: C26H24IN3O4
- Molecular Weight:569.39
-
Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Tetramethylrhodamine-5-iodoacetamide (0.5, 1.0, 2.0, 3.0, 4.0, 5.0, and 7.5 mM dye) shows different results for labeling carbonic anhydrase and myoglobin. At lower dye concentrations (up to 40:1 dye:thiol ratio) fluorescence labeling of only the carbonic anhydrase band is observed. However,as concentration increases, fluorescence labeling of both carbonic anhydrase and the lower myoglobin band is observed, indicating nonspecific labeling at these dye:thiol ratios[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 114458-99-0
-
Appearance Solid
-
Molecular Weight 569.39
-
Formula C26H24IN3O4
-
Color Brown to reddish brown
-
SMILES
CN(C1=CC2=[O+]C3=C(C=CC(N(C)C)=C3)C(C4=CC=C(NC(CI)=O)C=C4C([O-])=O)=C2C=C1)C
-
Synonyms
5-TMRIA
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 125 mg/mL (219.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (274 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7563 mL | 8.7813 mL | 17.5627 mL | 43.9066 mL |
| 5 mM | 0.3513 mL | 1.7563 mL | 3.5125 mL | 8.7813 mL | |
| 10 mM | 0.1756 mL | 0.8781 mL | 1.7563 mL | 4.3907 mL | |
| 15 mM | 0.1171 mL | 0.5854 mL | 1.1708 mL | 2.9271 mL | |
| 20 mM | 0.0878 mL | 0.4391 mL | 0.8781 mL | 2.1953 mL | |
| 25 mM | 0.0703 mL | 0.3513 mL | 0.7025 mL | 1.7563 mL | |
| 30 mM | 0.0585 mL | 0.2927 mL | 0.5854 mL | 1.4636 mL | |
| 40 mM | 0.0439 mL | 0.2195 mL | 0.4391 mL | 1.0977 mL | |
| 50 mM | 0.0351 mL | 0.1756 mL | 0.3513 mL | 0.8781 mL | |
| 60 mM | 0.0293 mL | 0.1464 mL | 0.2927 mL | 0.7318 mL | |
| 80 mM | 0.0220 mL | 0.1098 mL | 0.2195 mL | 0.5488 mL | |
| 100 mM | 0.0176 mL | 0.0878 mL | 0.1756 mL | 0.4391 mL |
Tetramethylrhodamine-5-iodoacetamide Related Classifications
HY-123749 Related Classifications
Powered by Bioz