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  4. Tetramethylrhodamine-5-iodoacetamide

Tetramethylrhodamine-5-iodoacetamide  (Synonyms: 5-TMRIA)

製品番号: HY-123749 純度: 86.61%
COA 取扱説明書 Technical Support

Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) is a thiol-selective reactive dye that is used to non-specifically label proteins via the cysteine residues. Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) can be used to covalently label DNA fragments.

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Tetramethylrhodamine-5-iodoacetamide

Tetramethylrhodamine-5-iodoacetamide 構造式

CAS 番号 : 114458-99-0

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 670 在庫あり
Solution
10 mM * 1 mL in DMSO USD 670 在庫あり
Solid
5 mg $535 在庫あり
10 mg $800 在庫あり
50 mg   お問い合わせ  
100 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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Based on 1 publication(s) in Google Scholar

Tetramethylrhodamine-5-iodoacetamide 関連抗体

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製品説明

Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) is a thiol-selective reactive dye that is used to non-specifically label proteins via the cysteine residues. Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) can be used to covalently label DNA fragments[1].

体外実験

Tetramethylrhodamine-5-iodoacetamide (0.5, 1.0, 2.0, 3.0, 4.0, 5.0, and 7.5 mM dye) shows different results for labeling carbonic anhydrase and myoglobin. At lower dye concentrations (up to 40:1 dye:thiol ratio) fluorescence labeling of only the carbonic anhydrase band is observed. However,as concentration increases, fluorescence labeling of both carbonic anhydrase and the lower myoglobin band is observed, indicating nonspecific labeling at these dye:thiol ratios[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Tetramethylrhodamine-5-iodoacetamide 関連抗体
分子量

569.39

分子式

C26H24IN3O4
CAS 番号
Appearance

Solid

Color

Brown to reddish brown

SMILES

CN(C1=CC2=[O+]C3=C(C=CC(N(C)C)=C3)C(C4=CC=C(NC(CI)=O)C=C4C([O-])=O)=C2C=C1)C
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶剤 & 溶解度
体外: 

DMSO : 125 mg/mL (219.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7563 mL 8.7813 mL 17.5627 mL
5 mM 0.3513 mL 1.7563 mL 3.5125 mL
10 mM 0.1756 mL 0.8781 mL 1.7563 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

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純度とドキュメンテーション
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.7563 mL 8.7813 mL 17.5627 mL 43.9066 mL
5 mM 0.3513 mL 1.7563 mL 3.5125 mL 8.7813 mL
10 mM 0.1756 mL 0.8781 mL 1.7563 mL 4.3907 mL
15 mM 0.1171 mL 0.5854 mL 1.1708 mL 2.9271 mL
20 mM 0.0878 mL 0.4391 mL 0.8781 mL 2.1953 mL
25 mM 0.0703 mL 0.3513 mL 0.7025 mL 1.7563 mL
30 mM 0.0585 mL 0.2927 mL 0.5854 mL 1.4636 mL
40 mM 0.0439 mL 0.2195 mL 0.4391 mL 1.0977 mL
50 mM 0.0351 mL 0.1756 mL 0.3513 mL 0.8781 mL
60 mM 0.0293 mL 0.1464 mL 0.2927 mL 0.7318 mL
80 mM 0.0220 mL 0.1098 mL 0.2195 mL 0.5488 mL
100 mM 0.0176 mL 0.0878 mL 0.1756 mL 0.4391 mL
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Tetramethylrhodamine-5-iodoacetamide Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tetramethylrhodamine-5-iodoacetamide114458-99-05-TMRIAFluorescent DyeInhibitorinhibitorinhibit

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製品名:
Tetramethylrhodamine-5-iodoacetamide
製品番号:
HY-123749
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