1. Dye Reagents
  2. Tetramethylrhodamine-5-iodoacetamide

Tetramethylrhodamine-5-iodoacetamide (Synonyms: 5-TMRIA)

Cat. No.: HY-123749
Handling Instructions

Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) is a thiol-selective reactive dye that is used to non-specifically label proteins via the cysteine residues. Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) can be used to covalently label DNA fragments.

For research use only. We do not sell to patients.

Tetramethylrhodamine-5-iodoacetamide Chemical Structure

Tetramethylrhodamine-5-iodoacetamide Chemical Structure

CAS No. : 114458-99-0

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Description

Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) is a thiol-selective reactive dye that is used to non-specifically label proteins via the cysteine residues. Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) can be used to covalently label DNA fragments[1].

In Vitro

Tetramethylrhodamine-5-iodoacetamide (0.5, 1.0, 2.0, 3.0, 4.0, 5.0, and 7.5 mM dye) shows different results for labeling carbonic anhydrase and myoglobin. At lower dye concentrations (up to 40:1 dye:thiol ratio) fluorescence labeling of only the carbonic anhydrase band is observed. However,as concentration increases, fluorescence labeling of both carbonic anhydrase and the lower myoglobin band is observed, indicating nonspecific labeling at these dye:thiol ratios[1].

Molecular Weight

569.39

Formula

C₂₆H₂₄IN₃O₄

CAS No.

114458-99-0

SMILES

CN(C1=CC2=[O+]C3=C(C=CC(N(C)C)=C3)C(C4=CC=C(NC(CI)=O)C=C4C([O-])=O)=C2C=C1)C

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Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

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Keywords:

Tetramethylrhodamine-5-iodoacetamide5-TMRIAOthersInhibitorinhibitorinhibit

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Tetramethylrhodamine-5-iodoacetamide
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