Mca-(ala7,lys(dnp)9)-bradykinin
Based on 1 Customer Validation
Mca-(ala7,lys(dnp)9)-bradykinin is a sensitive fluorogenic substrate for ECE-1 (endothelin-converting enzyme-1). The incorporation of a (7-methoxycoumarin-4-yl)acetyl (Mca) fluorescent group and a 2,4-dinitrophenyl (Dnp) quenching group has resulted in a large fluorescence increase upon substrate cleavage.
For research use only. We do not sell to patients.
- Purity: 99.93%
- CAS No.: 323577-36-2
- Formula: C66H81N15O19
- Molecular Weight:1388.44
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Storage:
Sealed storage, away from moisture and light, under nitrogen.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Mca-(ala7,lys(dnp)9)-bradykinin is also a substrate for neprilysin, but is hydrolyzed 10-fold more efficiently by ECE-1, making this substrate selective for ECE-1[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
460
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Chemical Information
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CAS No. 323577-36-2
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Appearance Solid
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Molecular Weight 1388.44
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Formula C66H81N15O19
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Color Light yellow to yellow
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Sequence Shortening
{MOCAc}-RPPGFSAFK-{DNP}
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light, under nitrogen
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
Solvent & Solubility
DMSO : 100 mg/mL (72.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.7202 mL | 3.6012 mL | 7.2023 mL | 18.0058 mL |
| 5 mM | 0.1440 mL | 0.7202 mL | 1.4405 mL | 3.6012 mL | |
| 10 mM | 0.0720 mL | 0.3601 mL | 0.7202 mL | 1.8006 mL | |
| 15 mM | 0.0480 mL | 0.2401 mL | 0.4802 mL | 1.2004 mL | |
| 20 mM | 0.0360 mL | 0.1801 mL | 0.3601 mL | 0.9003 mL | |
| 25 mM | 0.0288 mL | 0.1440 mL | 0.2881 mL | 0.7202 mL | |
| 30 mM | 0.0240 mL | 0.1200 mL | 0.2401 mL | 0.6002 mL | |
| 40 mM | 0.0180 mL | 0.0900 mL | 0.1801 mL | 0.4501 mL | |
| 50 mM | 0.0144 mL | 0.0720 mL | 0.1440 mL | 0.3601 mL | |
| 60 mM | 0.0120 mL | 0.0600 mL | 0.1200 mL | 0.3001 mL |
Mca-(ala7,lys(dnp)9)-bradykinin Related Classifications
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