Mca-(ala7,lys(dnp)9)-bradykinin

Name: Mca-(ala7,lys(dnp)9)-bradykinin
Brand: MedChemExpress
SKU: HY-P3749
Rating: 4.5 (1 reviews)

Cat. No.: HY-P3749 Purity: 99.90%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Mca-(ala7,lys(dnp)9)-bradykinin is a sensitive fluorogenic substrate for ECE-1 (endothelin-converting enzyme-1). The incorporation of a (7-methoxycoumarin-4-yl)acetyl (Mca) fluorescent group and a 2,4-dinitrophenyl (Dnp) quenching group has resulted in a large fluorescence increase upon substrate cleavage.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Mca-(ala7,lys(dnp)9)-bradykinin Chemical Structure

CAS No. : 323577-36-2

Size	Price	Stock	Quantity
1 mg	USD 220	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 650	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 1050	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Mca-(ala7,lys(dnp)9)-bradykinin is also a substrate for neprilysin, but is hydrolyzed 10-fold more efficiently by ECE-1, making this substrate selective for ECE-1^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

1388.44

Formula

C₆₆H₈₁N₁₅O₁₉

CAS No.

323577-36-2

Appearance

Solid

Color

Light yellow to yellow

Emission (Em)

460

Excitation (Ex)

380

Sequence Shortening

{MOCAc}-RPPGFSAFK-{DNP}

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture and light, under nitrogen

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (72.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.7202 mL	3.6012 mL	7.2023 mL
5 mM	0.1440 mL	0.7202 mL	1.4405 mL
10 mM	0.0720 mL	0.3601 mL	0.7202 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.90%

Select Batch:

Data Sheet (273 KB) SDS (252 KB)

COA (250 KB) LCMS (93 KB) Elemental Analysis Report (109 KB)

Handling Instructions (2659 KB)

References

[1]. Johnson GD, et al. Development of an internally quenched fluorescent substrate selective for endothelin-converting enzyme-1. Anal Biochem. 2000 Nov 1;286(1):112-8. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	0.7202 mL	3.6012 mL	7.2023 mL	18.0058 mL
	5 mM	0.1440 mL	0.7202 mL	1.4405 mL	3.6012 mL
	10 mM	0.0720 mL	0.3601 mL	0.7202 mL	1.8006 mL
	15 mM	0.0480 mL	0.2401 mL	0.4802 mL	1.2004 mL
	20 mM	0.0360 mL	0.1801 mL	0.3601 mL	0.9003 mL
	25 mM	0.0288 mL	0.1440 mL	0.2881 mL	0.7202 mL
	30 mM	0.0240 mL	0.1200 mL	0.2401 mL	0.6002 mL
	40 mM	0.0180 mL	0.0900 mL	0.1801 mL	0.4501 mL
	50 mM	0.0144 mL	0.0720 mL	0.1440 mL	0.3601 mL
	60 mM	0.0120 mL	0.0600 mL	0.1200 mL	0.3001 mL