1. Dye Reagents
  2. 6-CFDA

6-CFDA (Synonyms: 6-Carboxyfluorescein diacetate)

Cat. No.: HY-D0721 Purity: 95.09%
Handling Instructions

6-CFDA is fluorescent polyanionic probe, and its derivatives such as 2′,7′-bis (2-carboxyethyl)-5(6)-carboxyfluorescein are used to measure changes in intracellular pH (pHi) and processes such as dendrimer aggregation and absorption.

For research use only. We do not sell to patients.

6-CFDA Chemical Structure

6-CFDA Chemical Structure

CAS No. : 3348-03-6

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 79 In-stock
Estimated Time of Arrival: December 31
10 mg USD 72 In-stock
Estimated Time of Arrival: December 31
50 mg USD 96 In-stock
Estimated Time of Arrival: December 31
100 mg USD 168 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

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Description

6-CFDA is fluorescent polyanionic probe, and its derivatives such as 2′,7′-bis (2-carboxyethyl)-5(6)-carboxyfluorescein are used to measure changes in intracellular pH (pHi) and processes such as dendrimer aggregation and absorption.

Molecular Weight

460.39

Formula

C₂₅H₁₆O₉

CAS No.

3348-03-6

SMILES

CC(OC1=CC=C(C2(O3)C4=CC(C(O)=O)=CC=C4C3=O)C(OC5=C2C=CC(OC(C)=O)=C5)=C1)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (217.21 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1721 mL 10.8604 mL 21.7207 mL
5 mM 0.4344 mL 2.1721 mL 4.3441 mL
10 mM 0.2172 mL 1.0860 mL 2.1721 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (5.43 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (5.43 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (5.43 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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This equation is commonly abbreviated as: C1V1 = C2V2

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Keywords:

6-CFDA6-Carboxyfluorescein diacetateOthersInhibitorinhibitorinhibit

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6-CFDA
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HY-D0721
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