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  4. BODIPY FL alkyne

BODIPY FL alkyne  (Synonyms: BOD FL alkyne)

Cat. No.: HY-114354 Purity: 98.60%
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BODIPY (BOD) FL alkyne is an alkyne-containing BODIPY fluorophore derivative. BODIPY FL alkyne is a bioorthogonal labeling reagent with low toxicity and extremely low non-specific reactivity, and it is widely used in fluorescent bioimaging. BODIPY FL alkyne specifically labels azide groups on intracellular glycoconjugates mainly via strain-promoted azide-alkyne cycloaddition (SPAAC), or mediates site-specific conjugation with proteins such as IL-33, and supports positive cross-linking with other probes (e.g., DBCO-SCy5) for dual labeling. With the advantages of high specificity and low background interference, BODIPY FL alkyne can be used in the research of related diseases such as asthma, atopic dermatitis and inflammatory bowel disease.

For research use only. We do not sell to patients.

BODIPY FL alkyne

BODIPY FL alkyne Chemical Structure

CAS No. : 302795-84-2

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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BODIPY FL alkyne Related Antibodies

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Description

BODIPY (BOD) FL alkyne is an alkyne-containing BODIPY fluorophore derivative. BODIPY FL alkyne is a bioorthogonal labeling reagent with low toxicity and extremely low non-specific reactivity, and it is widely used in fluorescent bioimaging. BODIPY FL alkyne specifically labels azide groups on intracellular glycoconjugates mainly via strain-promoted azide-alkyne cycloaddition (SPAAC), or mediates site-specific conjugation with proteins such as IL-33, and supports positive cross-linking with other probes (e.g., DBCO-SCy5) for dual labeling. With the advantages of high specificity and low background interference, BODIPY FL alkyne can be used in the research of related diseases such as asthma, atopic dermatitis and inflammatory bowel disease[1][2][3].

In Vitro

BODIPY-based monofunctional Pt(II) conjugate 1 exhibits specific photocytotoxicity towards HeLa and MCF-7 cancer cells (IC50=13.1 μM and 7.6 μM, respectively) with lower toxicity to normal HBL-100 and L02 cells, and generates singlet oxygen with a quantum yield of 0.19[2].
BODIPY FL alkyne is an environmentally sensitive fluorogenic building block that shows an 8-fold increase in fluorescence emission in hydrophobic media[3].
BODIPY FL alkyne (1 μg/mL HpBARI_Hom2) labeled IL-33 enables wash-free, real-time imaging of ST2-mediated internalization in live HEK-Blue cells, with signals that are specifically blocked by the ST2 antagonist HpBARI_Hom2[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

BODIPY FL alkyne Related Antibodies

Immunofluorescence[3]

Cell Line: ST2-expressing HEK-Blue cells
Concentration: 1 μg/mL HpBARI_Hom2 (blockade)
Incubation Time: 0.5-5 min (time-course imaging); pre-incubation (blockade)
Result: Showed similar fluorescence intensity in cells before and after washing with PBS, indicating specific ST2-mediated internalization.
Time-course imaging detected ST2-mediated internalization within minutes.
Pre-incubation with HpBARI_Hom2 eliminated all green fluorescence signals.
Molecular Weight

329.15

Formula

C17H18BF2N3O
CAS No.
Appearance

Solid

Color

Light yellow to yellow

Emission (Em)

516
Excitation (Ex)

495
SMILES

O=C(NCC#C)CCC1=CC=C(C=C(C(C)=C2)[N]3=C2C)[N-]1[B+3]3([F-])[F-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (151.91 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0381 mL 15.1906 mL 30.3813 mL
5 mM 0.6076 mL 3.0381 mL 6.0763 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 98.60%

SDS (252 KB)

COA (243 KB) HNMR (251 KB) RP-HPLC (234 KB) MS (71 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.0381 mL 15.1906 mL 30.3813 mL 75.9532 mL
5 mM 0.6076 mL 3.0381 mL 6.0763 mL 15.1906 mL
10 mM 0.3038 mL 1.5191 mL 3.0381 mL 7.5953 mL
15 mM 0.2025 mL 1.0127 mL 2.0254 mL 5.0635 mL
20 mM 0.1519 mL 0.7595 mL 1.5191 mL 3.7977 mL
25 mM 0.1215 mL 0.6076 mL 1.2153 mL 3.0381 mL
30 mM 0.1013 mL 0.5064 mL 1.0127 mL 2.5318 mL
40 mM 0.0760 mL 0.3798 mL 0.7595 mL 1.8988 mL
50 mM 0.0608 mL 0.3038 mL 0.6076 mL 1.5191 mL
60 mM 0.0506 mL 0.2532 mL 0.5064 mL 1.2659 mL
80 mM 0.0380 mL 0.1899 mL 0.3798 mL 0.9494 mL
100 mM 0.0304 mL 0.1519 mL 0.3038 mL 0.7595 mL
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BODIPY FL alkyne Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BODIPY FL alkyne302795-84-2BOD FL alkyneFluorescent Dyemitochondriainflammatory bowel diseasesintracellular glycoconjugatesMCF-7 cellsHeLa cellsasthmaatopic dermatitisA549 cellsstrain-promoted azide-alkyne cycloadditioninterleukin IL-33Inhibitorinhibitorinhibit

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