BODIPY FL alkyne
Based on 1 Customer Validation
BODIPY (BOD) FL alkyne is an alkyne-containing BODIPY fluorophore derivative. BODIPY FL alkyne is a bioorthogonal labeling reagent with low toxicity and extremely low non-specific reactivity, and it is widely used in fluorescent bioimaging. BODIPY FL alkyne specifically labels azide groups on intracellular glycoconjugates mainly via strain-promoted azide-alkyne cycloaddition (SPAAC), or mediates site-specific conjugation with proteins such as IL-33, and supports positive cross-linking with other probes (e.g., DBCO-SCy5) for dual labeling. With the advantages of high specificity and low background interference, BODIPY FL alkyne can be used in the research of related diseases such as asthma, atopic dermatitis and inflammatory bowel disease.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit: 98.60%
- CAS. Nr.: 302795-84-2
- Formel: C17H18BF2N3O
- Molecular Weight:329.15
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Speicherung:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Biologische Aktivität
Beschreibung
In Vitro
BODIPY-based monofunctional Pt(II) conjugate 1 exhibits specific photocytotoxicity towards HeLa and MCF-7 cancer cells (IC50=13.1 μM and 7.6 μM, respectively) with lower toxicity to normal HBL-100 and L02 cells, and generates singlet oxygen with a quantum yield of 0.19[2].
BODIPY FL alkyne is an environmentally sensitive fluorogenic building block that shows an 8-fold increase in fluorescence emission in hydrophobic media[3].
BODIPY FL alkyne (1 μg/mL HpBARI_Hom2) labeled IL-33 enables wash-free, real-time imaging of ST2-mediated internalization in live HEK-Blue cells, with signals that are specifically blocked by the ST2 antagonist HpBARI_Hom2[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:ST2-expressing HEK-Blue cells
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Concentration:1 μg/mL HpBARI_Hom2 (blockade)
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Incubation Time:0.5-5 min (time-course imaging); pre-incubation (blockade)
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Result:Showed similar fluorescence intensity in cells before and after washing with PBS, indicating specific ST2-mediated internalization.
Time-course imaging detected ST2-mediated internalization within minutes.
Pre-incubation with HpBARI_Hom2 eliminated all green fluorescence signals.
Emission (Em)
516
Excitation (Ex)
495
Chemical Information
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CAS. Nr. 302795-84-2
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Appearance Solid
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Molecular Weight 329.15
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Formel C17H18BF2N3O
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Color Light yellow to yellow
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SMILES
O=C(NCC#C)CCC1=CC=C(C=C(C(C)=C2)[N]3=C2C)[N-]1[B+3]3([F-])[F-]
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Synonyms
BOD FL alkyne
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 50 mg/mL (151.91 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Reinheit & Dokumentation
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Data Sheet (271 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Verweise
[2]. Antina E, et al. BODIPY Conjugates as Functional Compounds for Medical Diagnostics and Treatment. Molecules. 2022;27(4):1396. Published 2022 Feb 18. [Content Brief]
[3]. Reese AE, et al. Inserting "OFF-to-ON" BODIPY Tags into Cytokines: A Fluorogenic Interleukin IL-33 for Real-Time Imaging of Immune Cells. ACS Cent Sci. 2023;10(1):143-154. Published 2023 Dec 20. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.0381 mL | 15.1906 mL | 30.3813 mL | 75.9532 mL |
| 5 mM | 0.6076 mL | 3.0381 mL | 6.0763 mL | 15.1906 mL | |
| 10 mM | 0.3038 mL | 1.5191 mL | 3.0381 mL | 7.5953 mL | |
| 15 mM | 0.2025 mL | 1.0127 mL | 2.0254 mL | 5.0635 mL | |
| 20 mM | 0.1519 mL | 0.7595 mL | 1.5191 mL | 3.7977 mL | |
| 25 mM | 0.1215 mL | 0.6076 mL | 1.2153 mL | 3.0381 mL | |
| 30 mM | 0.1013 mL | 0.5064 mL | 1.0127 mL | 2.5318 mL | |
| 40 mM | 0.0760 mL | 0.3798 mL | 0.7595 mL | 1.8988 mL | |
| 50 mM | 0.0608 mL | 0.3038 mL | 0.6076 mL | 1.5191 mL | |
| 60 mM | 0.0506 mL | 0.2532 mL | 0.5064 mL | 1.2659 mL | |
| 80 mM | 0.0380 mL | 0.1899 mL | 0.3798 mL | 0.9494 mL | |
| 100 mM | 0.0304 mL | 0.1519 mL | 0.3038 mL | 0.7595 mL |
BODIPY FL alkyne Related Classifications
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