1. Immunology/Inflammation
  2. COX

(+)-Catechin hydrate 

Cat. No.: HY-N0355 Purity: 99.22%
Handling Instructions

(+)-Catechin hydrate inhibits cyclooxygenase-1 (COX-1) with an IC50 of 1.4 μM.

For research use only. We do not sell to patients.

(+)-Catechin hydrate Chemical Structure

(+)-Catechin hydrate Chemical Structure

CAS No. : 225937-10-0

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10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
100 mg USD 50 In-stock
Estimated Time of Arrival: December 31
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Description

(+)-Catechin hydrate inhibits cyclooxygenase-1 (COX-1) with an IC50 of 1.4 μM.

IC50 & Target

COX-1

1.4 μM (IC50)

In Vitro

(+)-Catechin exhibits >95% inhibitory activity at 70 μg/mL against cyclooxygenase-1 (COX-1) with an IC50 of 1.4 μM[1]. A dose-dependent reduction in color is observed after 24 hours of treatment with (+)-Catechin, and 54.76% of the cells are dead at the highest concentration of (+)-Catechin tested (160 μg/mL) whereas the IC50 of (+)-Catechin is achieved at 127.62 μg/mL (+)-Catechin. A dose- and time-dependent increase in the induction of apoptosis is observed when MCF-7 cells are treated with (+)-Catechin. When compare to the control cells at 24 hours, 40.7 and 41.16% of the cells treated with 150 μg/mL and 300 μg/mL (+)-Catechin, respectively, undergo apoptosis. The expression levels of Caspase-3, -8, and -9 and p53 in MCF-7 cells treated with 150 μg/mL (+)-Catechin for 24 h increase by 5.81, 1.42, 3.29, and 2.68 fold, respectively, as compare to the levels in untreated control cells[2].

In Vivo

Animals treated with (+)-Catechin at the lowest tested dose, i.e., 50 mg/kg, p.o. have spent comparatively more time in exploring the novel object in the choice trial, however, the difference is not statistically significant. (+)-Catechin prevents the time-induced episodic memory deficits in a dose-dependent manner, the most effective being 200 mg/kg, p.o.. Treatment with (+)-Catechin prevents the rise in MPO level compare to DOX alone treatment group (21.98±9.44 and 36.76±4.39% in the hippocampus and the frontal cortex respectively)[3].

Clinical Trial
Solvent & Solubility
In Vitro: 

DMSO : 150 mg/mL (Need ultrasonic and warming)

References
Cell Assay
[2]

The Cell viability assay is performed to assess the toxicity of different concentrations of (+)-Catechin on MCF-7 cells. Briefly, MCF-7 cells (2×104 cells/well) are plated in 96-well plates and treated with 0 μg/mL (+)-Catechin and 160 μg/mL (+)-Catechin for 24 hours. Then, 40 μL of the Cell Titer Blue solution is directly added to the wells and incubated at 37°C for 6 hours. The fluorescence is recorded with a 560 nm/590 nm (excitation/emission) filter set using a microplate fluorescence reader, and the IC50 is calculated. Quadruplet samples are run for each concentration of (+)-Catechin in three independent experiments[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[3]

Rats[3]
Twelve weeks old, healthy male rats weighing 200 to 230 g are used in this study. Rats are divided into four experimental groups (n=9 each) for one vehicle and three groups of (+)-Catechin (three doses). The doses of (+)-Catechin are prepared at 50, 100, 200 mg/kg and administered orally for 7 days prior to and during the experimental trials. Episodic memory, the conscious memory of the past experiences is evaluated in this study[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Formula

C₁₅H₁₄O₆.xH₂O

CAS No.

225937-10-0

SMILES

OC1=CC(O)=C(C[[email protected]](O)[[email protected]@H](C2=CC=C(O)C(O)=C2)O3)C3=C1.[xH2O]

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
(+)-Catechin hydrate
Cat. No.:
HY-N0355
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(+)-Catechin hydrate

Cat. No.: HY-N0355