Amiloride
Based on 64 publication(s) in Google Scholar
Amiloride (MK-870) is an inhibitor of both epithelial sodium channel (ENaC) and urokinase-type plasminogen activator receptor (uTPA). Amiloride is a blocker of polycystin-2 (PC2; TRPP2) channel.
For research use only. We do not sell to patients.
- Purity: 99.96%
- CAS No.: 2609-46-3
- Formula: C6H8ClN7O
- Molecular Weight:229.63
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Publications Citing Use of MedChemExpress (MCE) Amiloride
More- Nat Nanotechnol. 2024 Dec;19(12):1903-1913. [Abstract]
- Cell Metab. 2022 Dec 6;34(12):2018-2035.e8. [Abstract]
- Nat Biomed Eng. 2024 Nov;8(11):1469-1482. [Abstract]
- Cell Mol Immunol. 2024 Aug;21(8):826-841. [Abstract]
- Adv Funct Mater. 2025 Aug 3.
- Adv Funct Mater. 2024 Oct 31.
- Mol Neurodegener. 2025 Jul 23;20(1):84. [Abstract]
- ACS Nano. 2025 Dec 26. [Abstract]
- ACS Nano. 2023 May 9;17(9):8141-8152. [Abstract]
- J Am Chem Soc. 2018 Dec 12;140(49):17234-17240. [Abstract]
- Acta Pharm Sin B. 2024 Dec;14(12):5418-5434. [Abstract]
- J Extracell Vesicles. 2026 Feb;15(2):e70238. [Abstract]
- Adv Sci (Weinh). 2025 May 14:e2406150. [Abstract]
- Adv Sci (Weinh). 2025 Apr 3:e2412356. [Abstract]
- Adv Sci (Weinh). 2024 Mar;11(9):e2307880. [Abstract]
- Theranostics. 2020 May 17;10(15):6581-6598. [Abstract]
- Theranostics. 2019 May 31;9(13):3732-3753. [Abstract]
- Biomaterials. 2026 Jan:324:123541. [Abstract]
- Biomaterials. 2022 May;284:121529. [Abstract]
- J Nanobiotechnology. 2020 Jan 31;18(1):26. [Abstract]
- Small. 2026 Jun 5:e74067. [Abstract]
- J Control Release. 2025 Nov 20:389:114450. [Abstract]
- J Control Release. 2024 Oct 26:S0168-3659(24)00725-9. [Abstract]
- J Hazard Mater. 2025 Dec 5:500:140498. [Abstract]
- Environ Sci Technol. 2021 Mar 2;55(5):3144-3155. [Abstract]
- Cell Rep Med. 2025 Jul 15;6(7):102208. [Abstract]
- Biosens Bioelectron. 2025 Jan 25:274:117184. [Abstract]
- Mater Today Bio. 2025 Mar 27:32:101712. [Abstract]
- Cell Mol Biol Lett. 2024 Dec 3;29(1):149. [Abstract]
- J Neuroinflammation. 2022 Feb 22;19(1):53. [Abstract]
- Food Chem. 2024 Sep 8;463(Pt 1):141196. [Abstract]
- Acta Biomater. 2025 Jul 1:201:171-186. [Abstract]
- Proc Natl Acad Sci U S A. 2026 Mar 31;123(13):e2520119123. [Abstract]
- Small Methods. 2021 Apr;5(4):e2001047. [Abstract]
- J Pharm Anal. 2025 Jul;15(7):101182. [Abstract]
- Cell Commun Signal. 2023 May 4;21(1):99. [Abstract]
- Int J Biol Macromol. 2024 Aug 5;277(Pt 3):134517. [Abstract]
- Int J Biol Macromol. 2022 May 15:207:559-569. [Abstract]
- Food Res Int. 2025 Dec 20;226:118114.
- Sci Total Environ. 2021 Jul 20:779:146523. [Abstract]
- Br J Pharmacol. 2020 Aug;177(15):3473-3488. [Abstract]
- Cell Rep. 2022 May 31;39(9):110880. [Abstract]
- Anal Chem. 2026 Feb 10;98(5):3577-3587. [Abstract]
- Anal Chem. 2025 Jun 17;97(23):12026-12034. [Abstract]
- J Cell Biol. 2025 May 5;224(5):e202405060. [Abstract]
- Transl Res. 2024 Jan:263:53-72. [Abstract]
- J Ethnopharmacol. 2022 Jun 28:292:115171. [Abstract]
- Commun Biol. 2022 Nov 14;5(1):1248. [Abstract]
- J Zhejiang Univ Sci B. 2025 May 28;26(5):461-476. [Abstract]
- mBio. 2025 Mar 12;16(3):e0378724. [Abstract]
- Mol Pharm. 2025 Apr 7;22(4):2182-2192. [Abstract]
- FASEB J. 2025 Jan 15;39(1):e70298. [Abstract]
- iScience. 2022 Oct 19;25(12):105403. [Abstract]
- Microb Pathog. 2019 Jul 18:103638. [Abstract]
- J Leukoc Biol. 2024 Jul 16:qiae159. [Abstract]
- J Pharm Biomed Anal. 2021 Feb 20:195:113870. [Abstract]
- Nanotechnology. 2018 Feb 23;29(8):085101. [Abstract]
- Cell Physiol Biochem. 2018;51(6):2564-2574. [Abstract]
- Am J Transl Res. 2021 Jul 15;13(7):7538-7555. [Abstract]
- NPJ Biomed Innov. 2026 May 22;3(1):33. [Abstract]
- Res Sq. 2026 Feb 5.
- Res Sq. 2025 Nov 16.
- SSRN. 2025 Aug 21.
- University of Toronto. 2025.
Customer Validation & Images
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IF
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WB
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Cell Imaging/Staining
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Flow Cytometry
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Cell Imaging/Staining
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK293 | IC50 |
>100 μM
Compound: 1
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Cytotoxicity against human HEK293 cells measured after 48 hrs by MTS assay
Cytotoxicity against human HEK293 cells measured after 48 hrs by MTS assay
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[PMID: 31679971] |
| HEK293 | IC50 |
57 μM
Compound: amiloride
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Inhibition of 4-(4-(dimethylamino)styryl)-N-methylpyridinium uptake at human OCT1 expressed in HEK293 cells by confocal microscopy
Inhibition of 4-(4-(dimethylamino)styryl)-N-methylpyridinium uptake at human OCT1 expressed in HEK293 cells by confocal microscopy
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[PMID: 18788725] |
| HepG2 | IC50 |
>100 μM
Compound: 1
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Cytotoxicity against human HepG2 cells measured after 48 hrs by MTS assay
Cytotoxicity against human HepG2 cells measured after 48 hrs by MTS assay
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[PMID: 31679971] |
| HT-1080 | IC50 |
>100 μM
Compound: 1
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Cytotoxicity against human HT1080 cells measured after 48 hrs by MTS assay
Cytotoxicity against human HT1080 cells measured after 48 hrs by MTS assay
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[PMID: 31679971] |
| MDA-MB-231 | IC50 |
>100 μM
Compound: 1
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Cytotoxicity against human MDA-MB-231 cells measured after 48 hrs by MTS assay
Cytotoxicity against human MDA-MB-231 cells measured after 48 hrs by MTS assay
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[PMID: 31679971] |
Amiloride blocks δβγ channels with an IC50 of 2.6 μM (58, 71, 75, 134, 148). The Ki of amiloride for δβγ ENaC is 26-fold that of αβγ channels (0.1 μM for αβγ ENaC). Amiloride blockade of δβγ ENaC is much more voltage dependent compared with the αβγ channel. The Ki of amiloride for δαβγ channels is 920 and 13.7 μM at -120 and +80 mV, respectively, which significantly differs from that of both αβγ and δβγ channels[1]. Amiloride is a relatively selective inhibitor of the epithelial sodium channel (ENaC) with an IC50 (the concentration required to reach 50% inhibition of an ion channel) in the concentration range of 0.1 to 0.5 μM. Amiloride is a relatively poor inhibitor of the the Na+/H+ exchanger (NHE) with an IC50 as low as 3 μM in the presence of a low external [Na+] but as high as 1 mM in the presence of a high [Na+]. Amiloride is an even weaker inhibitor of the Na+/Ca2+ exchanger (NCX), with an IC50 of 1 mM. Amiloride (1 μM) and submicromolar doses of Benzamil (30 nM), doses known to inhibit the ENaC, inhibit the myogenic vasoconstriction response to increasing perfusion pressure by blocking the activity of ENaC proteins. Amiloride completely inhibits Na+ influx in doses known to be relatively specific for ENaC (1.5 μM) in vascular smooth muscle cells (VSMC)[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2609-46-3
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Appearance Solid
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Molecular Weight 229.63
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Formula C6H8ClN7O
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Color Off-white to light yellow
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SMILES
O=C(C1=NC(Cl)=C(N)N=C1N)NC(N)=N
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Synonyms
MK-870
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (64)
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Journal Impact Factor
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Most Recent
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Nat Nanotechnol
Metal-ion-chelating phenylalanine nanostructures reverse immune dysfunction and sensitize breast tumour to immune checkpoint blockade. [Abstract]2024 Dec;19(12):1903-1913. PMID: 39187583 -
Cell Metab
Cancer cell-intrinsic XBP1 drives immunosuppressive reprogramming of intratumoral myeloid cells by promoting cholesterol production. [Abstract]2022 Dec 6;34(12):2018-2035.e8. PMID: 36351432
Amiloride purchased from MedChemExpress. Usage Cited in: Cell Metab. 2022 Dec 6;34(12):2018-2035.e8. [Abstract]
Bone marrow cells cultured in medium containing 10 ng/mL GM-CSF for 3 days were treated with 2 μg PKH67-labeled sEVs with or without Amiloride (10 μM), Chlorpromazine (10 μM), or EIPA (5 μM) for 1 h. The median fluorescence intensity (MFI) of PKH67 was assessed by flow cytometry.
Amiloride purchased from MedChemExpress. Usage Cited in: Cell Metab. 2022 Dec 6;34(12):2018-2035.e8. [Abstract]
Amiloride hydrochloride (10 μM; 3 d) crippled the effect of shNT cell-derived sEVs in elevating MDSCs to the level of shXBP cell-generated sEVs.
Amiloride purchased from MedChemExpress. Usage Cited in: Cell Metab. 2022 Dec 6;34(12):2018-2035.e8. [Abstract]
Amiloride hydrochloride (10 μM; 3 d) decreased the STAT3 phosphorylation level in MDSCs.
Amiloride purchased from MedChemExpress. Usage Cited in: Cell Metab. 2022 Dec 6;34(12):2018-2035.e8. [Abstract]
Amiloride hydrochloride (10 μM; 3 d) decreased the iNOS mRNA level in MDSCs.
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Nat Biomed Eng
In situ formation of biomolecular condensates as intracellular drug reservoirs for augmenting chemotherapy. [Abstract]2024 Nov;8(11):1469-1482. PMID: 39271933 -
Cell Mol Immunol
Depleting profibrotic macrophages using bioactivated in vivo assembly peptides ameliorates kidney fibrosis. [Abstract]2024 Aug;21(8):826-841. PMID: 38871810 -
Amiloride purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2025 Aug 3.
Typical confocal images of RAW 264.7 cells incubated with 100 µg/mL FITC/M-BSA@PSiNPs for 6 h, in the absence or presence of Amiloride（50 μM; 6 h).
Amiloride purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2025 Aug 3.
The inhibitory effects of Pitstop 2, MβCD, Amiloride (50 μM; 6 h) and CytoD on the expression of IL-1β, IL-6, CCL2, IFN-β, CXCL10 and TNF-α genes in RAW 264.7 cells.
Amiloride purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2025 Aug 3.
Typical confocal images of RAW 264.7 cells incubated with 100 μg/mL FITC/M-BSA@PSiNPs for 6 h, in the absence or presence of 50 μmol/L Pitstop 2, 100 μmol/L Methyl-β-cyclodextrin (MβCD), 50 μmol/L amiloride, or 1 μmol/L CytoD (scale bar = 40 μm).
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Mol Neurodegener
Endothelium-specific endoglin triggers astrocyte reactivity via extracellular vesicles in a mouse model of Alzheimer's disease. [Abstract]2025 Jul 23;20(1):84. PMID: 40702549 -
ACS Nano
Redox-Responsive Peptide Coacervates for Enhanced mRNA Delivery and Intracellular Release. [Abstract]2025 Dec 26. PMID: 41451635 -
ACS Nano
Enantioselective Degrader for Elimination of Extracellular Aggregation-Prone Proteins hIAPP Associated with Type 2 Diabetes. [Abstract]2023 May 9;17(9):8141-8152. PMID: 37057955 -
J Am Chem Soc
2018 Dec 12;140(49):17234-17240. PMID: 30398334 -
Acta Pharm Sin B
Development of a bacteria-nanosapper for the active delivery of ZIF-8 particles containing therapeutic genes for cancer immune therapy. [Abstract]2024 Dec;14(12):5418-5434. PMID: 39807327
Amiloride purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2024 Dec;14(12):5418-5434. [Abstract]
Amiloride hydrochloride (450 μM; 4 h) profoundly impaired the uptake rates of Il2/ZIF-8@Salmonella in RAW264.7 cells.
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J Extracell Vesicles
Extracellular Vesicles Coordinate Bacterial Cloaking in Lung Epithelial Cells to Alleviate Acute Inflammatory Injury. [Abstract]2026 Feb;15(2):e70238. PMID: 41656978 -
Adv Sci (Weinh)
Lipid Droplet-Derived Biomimetic Nanocarriers for the Enhancement of Porcine Intermuscular Fat Content. [Abstract]2025 May 14:e2406150. PMID: 40365640 -
Adv Sci (Weinh)
Screening and Identification of Novel DNA Aptamer for Targeted Delivery to Injured Podocytes in Glomerular Diseases. [Abstract]2025 Apr 3:e2412356. PMID: 40178289 -
Adv Sci (Weinh)
Pharmaceutical Manipulation of Mitochondrial F0F1-ATP Synthase Enables Imaging and Protection of Myocardial Ischemia/Reperfusion Injury Through Stress-induced Selective Enrichment. [Abstract]2024 Mar;11(9):e2307880. PMID: 38093654
Amiloride purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Mar;11(9):e2307880. [Abstract]
Representative images and quantitative data of the NIR fluorescent signals in H9C2 cells exposed to IR‐780 pre‐treatment with ice condition, Amiloride (75 µg/mL; 30 min).
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Theranostics
Proinflammatory macrophage-derived microvesicles exhibit tumor tropism dependent on CCL2/CCR2 signaling axis and promote drug delivery via SNARE-mediated membrane fusion. [Abstract]2020 May 17;10(15):6581-6598. PMID: 32550891 -
Theranostics
A Multifunctional Nanotherapy for Targeted Treatment of Colon Cancer by Simultaneously Regulating Tumor Microenvironment. [Abstract]2019 May 31;9(13):3732-3753. PMID: 31281510 -
Biomaterials
Biodegradable polymers with tertiary amines enhance mRNA delivery of lipid nanoparticles via improved endosomal escape. [Abstract]2026 Jan:324:123541. PMID: 40644791 -
Biomaterials
Targeted immunomodulation therapy for cardiac repair by platelet membrane engineering extracellular vesicles via hitching peripheral monocytes. [Abstract]2022 May;284:121529. PMID: 35447403 -
J Nanobiotechnology
Enhance transgene responses through improving cellular uptake and intracellular trafficking by bio-inspired non-viral vectors. [Abstract]2020 Jan 31;18(1):26. PMID: 32005170 -
Small
Self-Assembling Cationic Lipopeptides for the Construction of Functional Vesicular Gene-Delivery Systems. [Abstract]2026 Jun 5:e74067. PMID: 42246404 -
J Control Release
Inducing potent ferroptosis in cancer cells by manipulation of ferritinophagy with a ferritin-targeted ATTEC. [Abstract]2025 Nov 20:389:114450. PMID: 41274571 -
J Control Release
2024 Oct 26:S0168-3659(24)00725-9. PMID: 39490420 -
J Hazard Mater
CYP450 activation and mitophagy induction mediate Ti3C2 MXene detoxification in RAW 264.7 cells. [Abstract]2025 Dec 5:500:140498. PMID: 41252987 -
Environ Sci Technol
Graphene Quantum Dots Disrupt Embryonic Stem Cell Differentiation by Interfering with the Methylation Level of Sox 2. [Abstract]2021 Mar 2;55(5):3144-3155. PMID: 33569944 -
Cell Rep Med
CLDN4 palmitoylation promotes hepatic-to-biliary lineage transition and lenvatinib resistance in hepatocellular carcinoma. [Abstract]2025 Jul 15;6(7):102208. PMID: 40592346 -
Biosens Bioelectron
Cathepsin B dependent activatable trigger fluorophore (CAT-Fluor) for in situ functional imaging of antibody-drug conjugates. [Abstract]2025 Jan 25:274:117184. PMID: 39879789 -
Mater Today Bio
Metal-organic framework-based delivery systems as nanovaccine for enhancing immunity against porcine circovirus type 2. [Abstract]2025 Mar 27:32:101712. PMID: 40230641 -
Cell Mol Biol Lett
Acid-sensing ion channel-1 contributes to the failure of myelin sheath regeneration following spinal cord injury by transcellular delivery of PGE2. [Abstract]2024 Dec 3;29(1):149. PMID: 39627718 -
J Neuroinflammation
α-Synuclein-containing erythrocytic extracellular vesicles: essential contributors to hyperactivation of monocytes in Parkinson's disease. [Abstract]2022 Feb 22;19(1):53. PMID: 35193594 -
Food Chem
Absorption and transport mechanism of colloidal nanoparticles (CNPs) in lamb soup based on Caco-2 cell. [Abstract]2024 Sep 8;463(Pt 1):141196. PMID: 39260179 -
Acta Biomater
Hydrogel system with growth cone-targeted hydroxyapatite nanorods: Regulating calcium signals for peripheral nerve injury repair. [Abstract]2025 Jul 1:201:171-186. PMID: 40419070 -
Proc Natl Acad Sci U S A
Endogenous ATP-powered nanomotors directing neural stem cell differentiation for Parkinson's disease treatment. [Abstract]2026 Mar 31;123(13):e2520119123. PMID: 41880568 -
Small Methods
2021 Apr;5(4):e2001047. PMID: 34927842 -
J Pharm Anal
Effect of cholesterol on distribution, cell uptake, and protein corona of lipid microspheres at sites of cardiovascular inflammatory injury. [Abstract]2025 Jul;15(7):101182. PMID: 40735031 -
Cell Commun Signal
Promoting AMPK/SR-A1-mediated clearance of HMGB1 attenuates chemotherapy-induced peripheral neuropathy. [Abstract]2023 May 4;21(1):99. PMID: 37143083 -
Int J Biol Macromol
Growth inhibition of pancreatic cancer by targeted delivery of gemcitabine via fucoidan-coated pH-sensitive liposomes. [Abstract]2024 Aug 5;277(Pt 3):134517. PMID: 39111497 -
Int J Biol Macromol
Polyethylenimine-coated PLGA nanoparticles containing Angelica sinensis polysaccharide promote dendritic cells activation and associated molecular mechanisms. [Abstract]2022 May 15:207:559-569. PMID: 35288164 -
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Sci Total Environ
2021 Jul 20:779:146523. PMID: 34030247 -
Br J Pharmacol
An orally available hypoglycaemic peptide taken up by caveolae transcytosis displays improved hypoglycaemic effects and body weight control in db/db mice. [Abstract]2020 Aug;177(15):3473-3488. PMID: 32293707 -
Cell Rep
LL-37 transports immunoreactive cGAMP to activate STING signaling and enhance interferon-mediated host antiviral immunity. [Abstract]2022 May 31;39(9):110880. PMID: 35649354 -
Anal Chem
Fluorogenic Peptide-Modulated 2D ZnO/MnO2 Nanosheet Heterostructure Enables Monitoring of Tumor Early Response to Immunotherapy. [Abstract]2026 Feb 10;98(5):3577-3587. PMID: 41603436 -
Anal Chem
Unveiling the Spatiotemporal Behavior of Chiral Quantum Dots During Cellular Endocytosis. [Abstract]2025 Jun 17;97(23):12026-12034. PMID: 40470594 -
J Cell Biol
2025 May 5;224(5):e202405060. PMID: 40136051 -
Transl Res
Employing the sustained-release properties of poly(lactic-co-glycolic acid) nanoparticles to reveal a novel mechanism of sodium-hydrogen exchanger 1 in neuropathic pain. [Abstract]2024 Jan:263:53-72. PMID: 37678757 -
J Ethnopharmacol
Screening bioactive compounds from Danggui-shaoyao-san for treating sodium retention in nephrotic syndrome using bio-affinity ultrafiltration. [Abstract]2022 Jun 28:292:115171. PMID: 35259444 -
Commun Biol
2022 Nov 14;5(1):1248. PMID: 36376440 -
J Zhejiang Univ Sci B
Pharmacological inhibition of ENaC or NCX can attenuate hepatic ischemia-reperfusion injury exacerbated by hypernatremia. [Abstract]2025 May 28;26(5):461-476. PMID: 40436642 -
mBio
2025 Mar 12;16(3):e0378724. PMID: 39964166 -
Mol Pharm
2025 Apr 7;22(4):2182-2192. PMID: 40032688 -
FASEB J
Inhibition of ASIC1a reduces ferroptosis in rheumatoid arthritis articular chondrocytes via the p53/NRF2/SLC7A11 pathway. [Abstract]2025 Jan 15;39(1):e70298. PMID: 39760183 -
iScience
Renal lysophospholipase A1 contributes to Enterococcus faecalis-induced hypertension by enhancing sodium reabsorption. [Abstract]2022 Oct 19;25(12):105403. PMID: 36419851 -
Microb Pathog
Antiviral effect of amiloride on replication of foot and mouth disease virus in cell culture. [Abstract]2019 Jul 18:103638. PMID: 31326561
Amiloride purchased from MedChemExpress. Usage Cited in: Microb Pathog. 2019 Jul 18:103638. [Abstract]
The viral mRNA and VP1 protein levels in IBRS-2 cells treated with different concentrations of Amiloride are determined by qPCR and Western Blot, respectively. ‘‘VC’’ indicates that the cells that are not treated with Amiloride.
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J Leukoc Biol
Combination of microparticles vaccine with MSI-1436 exerts a strong immune response for hepatocellular carcinoma. [Abstract]2024 Jul 16:qiae159. PMID: 39012079 -
J Pharm Biomed Anal
Screening method of mildronate and over 300 doping agents by reversed-phase liquid chromatography-high resolution mass spectrometry. [Abstract]2021 Feb 20:195:113870. PMID: 33453569 -
Nanotechnology
Construction and evaluation of BSA-CaP nanomaterials with enhanced transgene performance via biocorona-inspired caveolae-mediated endocytosis. [Abstract]2018 Feb 23;29(8):085101. PMID: 29256442 -
Cell Physiol Biochem
Characterization of a DNA Aptamer for Ovarian Cancer Clinical Tissue Recognition and in Vivo Imaging. [Abstract]2018;51(6):2564-2574. PMID: 30562733 -
Am J Transl Res
Knockdown of VDAC1 alleviates the cognitive dysfunction secondary to sepsis-associated encephalopathy. [Abstract]2021 Jul 15;13(7):7538-7555. PMID: 34377234 -
NPJ Biomed Innov
2026 May 22;3(1):33. PMID: 42174191 -
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Solvent & Solubility
DMSO : 100 mg/mL (435.48 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.89 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.89 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (420 KB)
- English - EN (420 KB)
- Français - FR (420 KB)
- Deutsch - DE (420 KB)
- Norwegian - NO (420 KB)
- Español - ES (420 KB)
- Swedish - SV (420 KB)
- Italian - IT (420 KB)
- Portuguese - PT (420 KB)
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Handling Instructions (2659 KB)
References
[1]. Ji, H.L., et al. delta ENaC: a novel divergent amiloride-inhibitable sodium channel. Am J Physiol Lung Cell Mol Physiol, 2012. 303(12): p. L1013-26. [Content Brief]
[2]. Teiwes J, et al. Epithelial sodium channel inhibition in cardiovascular disease. A potential role for amiloride. Am J Hypertens. 2007 Jan;20(1):109-17. [Content Brief]
[3]. Giamarchi A, et al. A polycystin-2 (TRPP2) dimerization domain essential for the function of heteromeric polycystin complexes. EMBO J. 2010 Apr 7;29(7):1176-91. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.3548 mL | 21.7741 mL | 43.5481 mL | 108.8703 mL |
| 5 mM | 0.8710 mL | 4.3548 mL | 8.7096 mL | 21.7741 mL | |
| 10 mM | 0.4355 mL | 2.1774 mL | 4.3548 mL | 10.8870 mL | |
| 15 mM | 0.2903 mL | 1.4516 mL | 2.9032 mL | 7.2580 mL | |
| 20 mM | 0.2177 mL | 1.0887 mL | 2.1774 mL | 5.4435 mL | |
| 25 mM | 0.1742 mL | 0.8710 mL | 1.7419 mL | 4.3548 mL | |
| 30 mM | 0.1452 mL | 0.7258 mL | 1.4516 mL | 3.6290 mL | |
| 40 mM | 0.1089 mL | 0.5444 mL | 1.0887 mL | 2.7218 mL | |
| 50 mM | 0.0871 mL | 0.4355 mL | 0.8710 mL | 2.1774 mL | |
| 60 mM | 0.0726 mL | 0.3629 mL | 0.7258 mL | 1.8145 mL | |
| 80 mM | 0.0544 mL | 0.2722 mL | 0.5444 mL | 1.3609 mL | |
| 100 mM | 0.0435 mL | 0.2177 mL | 0.4355 mL | 1.0887 mL |
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