2. Membrane Transporter/Ion Channel Apoptosis Neuronal Signaling
  3. Sodium Channel Apoptosis TRP Channel
  4. Amiloride hydrochloride dihydrate

Amiloride hydrochloride dihydrate  (Synonyms: MK-870 hydrochloride dihydrate)

Cat. No.: HY-B0285B Purity: 99.70%
COA Handling Instructions

Amiloride hydrochloride dihydrate (MK-870 hydrochloride dihydrate) is an inhibitor of both epithelial sodium channel (ENaC) and urokinase-type plasminogen activator receptor (uTPA). Amiloride hydrochloride dihydrate is a blocker of polycystin-2 (PC2; TRPP2) channel.

For research use only. We do not sell to patients.

Amiloride hydrochloride dihydrate Chemical Structure

Amiloride hydrochloride dihydrate Chemical Structure

CAS No. : 17440-83-4

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Solution
10 mM * 1 mL in DMSO USD 73 In-stock
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Customer Review

Based on 25 publication(s) in Google Scholar

Other Forms of Amiloride hydrochloride dihydrate:

Amiloride hydrochloride dihydrate Related Antibodies

Top Publications Citing Use of Products

    Amiloride hydrochloride dihydrate purchased from MCE. Usage Cited in: Microb Pathog. 2019 Jul 18:103638.   [Abstract]

    The viral mRNA and VP1 protein levels in IBRS-2 cells treated with different concentrations of Amiloride are determined by qPCR and Western Blot, respectively. ‘‘VC’’ indicates that the cells that are not treated with Amiloride.

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    • Biological Activity

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    • References

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    Description

    Amiloride hydrochloride dihydrate (MK-870 hydrochloride dihydrate) is an inhibitor of both epithelial sodium channel (ENaC[1]) and urokinase-type plasminogen activator receptor (uTPA[2]). Amiloride hydrochloride dihydrate is a blocker of polycystin-2 (PC2; TRPP2[3]) channel.

    IC50 & Target

    ENaC[1]; uTPA[2]; polycystin-2(TRPP2)[3]
    In Vivo

    Amiloride hydrochloride dihydrate is a potent epithelial sodium channels (ENaCs) blocker. Amiloride is highly concentrated in the plasma 15 to 30 minutes following the injections. 2 mg/kg dose of Amiloride hydrochloride dihydrate has no effect on blood pressure, heart rate, mesenteric vascular resistance, or hindquarters vascular resistance as compare to the baseline measurements (n=7). Over a 2 hour period, Amiloride hydrochloride dihydrate elicits negligible responses in arterial pressure (-1±1 mmHg) and heart rate (-10±6 bpm/min) as compare to baseline levels. Results show an Amiloride hydrochloride dihydrate dose-related response pattern for the c-Fos activation in the area postrema (AP). Even at the lowest dose of Amiloride hydrochloride dihydrate (0.1 mg/kg), the number of c-Fos labeled neurons in the AP is statistically different from the control rats at a p<0.01 level[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    Molecular Weight

    302.12

    Appearance

    Solid

    Formula

    C6H13Cl2N7O3
    CAS No.
    SMILES

    O=C(C1=NC(Cl)=C(N)N=C1N)NC(N)=N.[H]Cl.O.O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 83.33 mg/mL (275.82 mM; ultrasonic and warming and heat to 60°C)

    H2O : 2 mg/mL (6.62 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.3099 mL 16.5497 mL 33.0994 mL
    5 mM 0.6620 mL 3.3099 mL 6.6199 mL
    10 mM 0.3310 mL 1.6550 mL 3.3099 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (8.27 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (8.27 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.08 mg/mL (6.88 mM); Clear solution

    *All of the co-solvents are available by MCE.
    Purity & Documentation

    Purity: 99.70%

    COA (251 KB) HNMR (255 KB) LCMS (255 KB)

    Handling Instructions (2659 KB)
    References
    Animal Administration
    [1]

    The experiments are performed on male and female Sprague-Dawley rats that have been maintained in a heat controlled room maintained at 23° C with a 12h/12h light-dark schedule. A total of 116 adult rats are injected i.p. with Amiloride hydrochloride dihydrate (0.1, 0.5, and 2.0 mg/kg). After 15, 30, 45, or 120 min post-injection, the rats are anesthetized with 8% chloral hydrate, the chest is opened, and blood samples are obtained by cardiac puncture. The blood is centrifuged at 5000 RPM for 5 min, and the plasma (1.5 mL) separated and stored at -80° C[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References
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    Amiloride hydrochloride dihydrate Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Keywords:

    Amiloride hydrochloride dihydrate17440-83-4MK-870 hydrochloride dihydrateSodium ChannelApoptosisTRP ChannelNa channelsNa+ channelsTransient receptor potential channelsInhibitorinhibitorinhibit

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