Antiviral effect of amiloride on replication of foot and mouth disease virus in cell culture

  • Microb Pathog. 2019 Oct;135:103638. doi: 10.1016/j.micpath.2019.103638.
Mei-Jiao Gong  1 ,  Yan-Yan Chang  1 ,  Jun-Jun Shao  1 ,  Shi-Fang Li  1 ,  Yong-Guang Zhang  1 ,  Hui-Yun Chang  2
Affiliations
  • 1. State Key Laboratory of Veterinary Etiological Biology, OIE/National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, China.
  • 2. State Key Laboratory of Veterinary Etiological Biology, OIE/National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, China. Electronic address: [email protected].
PMID: 31326561 DOI: 10.1016/j.micpath.2019.103638
Abstract

Recently, amiloride was shown to potently suppress Coxsackievirus B3 (CVB3) replication. In the current study, we investigated whether amiloride could also exhibit Antiviral activity against foot-and-mouth disease virus (FMDV), which belongs to the same family (Picornaviridae) as CVB3. We found that amiloride exerted Antiviral activity in a dose-dependent manner against two strains of FMDV in IBRS-2 cells, with slight cytotoxicity at 1000 μM. Besides, amiloride did not inhibit the attachment and entry of FMDV in IBRS-2 cells, but prevented early viral replication. These data implied that amiloride could be a promising candidate for further research as a potential Antiviral drug against FMDV Infection.

Keywords
Amiloride; Antiviral activity; FMDV; In vitro.
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