2. TGF-beta/Smad
  3. TGF-β Receptor
  4. BMP signaling agonist sb4

Sb4  (Synonyms: BMP signaling agonist sb4)

製品番号: HY-124697 純度: 99.98%
COA 取扱説明書 Technical Support

BMP signaling agonist sb4 is a potent benzoxazole bone morphogenetic protein 4 (BMP4) signaling agonist with a EC50 value of 74 nM, activates BMP signaling by stabilizing intracellular p-SMAD-1/5/9. BMP signaling agonist sb4 activates BMP4 target genes (inhibitors of DNA binding,?Id1?and?Id3) canonical BMP signaling.

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CAS 番号 : 100874-08-6

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 55 在庫あり
Solution
10 mM * 1 mL in DMSO USD 55 在庫あり
Solid
5 mg $50 在庫あり
10 mg $70 在庫あり
50 mg $160 在庫あり
100 mg $250 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 8 publication(s) in Google Scholar

Sb4 関連抗体

Top Publications Citing Use of Products

    BMP signaling agonist sb4 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jul 1;16(1):5470.  [Abstract]

    Percentage survival of mice after receiving 8.0 Gy of IR and different dosages of SB4 (1, 2.5, 5 mg/kg, i.p.).

    BMP signaling agonist sb4 purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2024 Nov 14;10(1):468.  [Abstract]

    mRNA expression levels of key genes in stem cell pluripotency (differentiation day 0), mesoderm (differentiation day 2), and intermediate mesoderm cells (differentiation day 4–6) by using qRT-PCR analysis under different combinations of SB4 (1, 10 μM) compound and/or BMP4.

    BMP signaling agonist sb4 purchased from MedChemExpress. Usage Cited in: ACS Pharmacol Transl Sci. 2024 Jun 24;7(7):1951-1970.  [Abstract]

    Inhibitory effect of BMPSB4 (0, 10, 20, 40, 80 μM,24-96 h) on AtT20/D16v-F2 cells showed dose-dependent and time-dependent effects. Cell viability was detected via the CCK-8 assay.

    BMP signaling agonist sb4 purchased from MedChemExpress. Usage Cited in: ACS Pharmacol Transl Sci. 2024 Jun 24;7(7):1951-1970.  [Abstract]

    Western blot assay showed a concentration-dependent phosphorylation of SMAD1/5/9 by BMPSB4 (40 μM).

    BMP signaling agonist sb4 purchased from MedChemExpress. Usage Cited in: ACS Pharmacol Transl Sci. 2024 Jun 24;7(7):1951-1970.  [Abstract]

    BMPSB4 (40, 80 μM) induced apoptosis of AtT20/D16v-F2 cells and was not affected by NOGGIN.

    TGF-β Receptor アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    ALK1 ALK2 ALK3 ALK4 ALK5 ALK6 ALK7 TGFβR2 ACVR2A ACVR2B BMP

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    製品説明

    BMP signaling agonist sb4 is a potent benzoxazole bone morphogenetic protein 4 (BMP4) signaling agonist with a EC50 value of 74 nM, activates BMP signaling by stabilizing intracellular p-SMAD-1/5/9. BMP signaling agonist sb4 activates BMP4 target genes (inhibitors of DNA binding,?Id1?and?Id3) canonical BMP signaling[1].

    IC50 & Target

    IC50: BMP4 signal[1]
    体外実験

    BMP signaling agonist sb4 (0.05 μM-1 μM; 24 hours) induces phosphorylated SMAD-1/5/9 in a dose-dependent manner in PRECs cells under serum-starved conitions[1].
    BMP signaling agonist sb4 (1 μM; 0-60 mins) enhances the efficacy of signaling at each concentration of rhBMP4 tested. This effect is most pronounced at low concentrations of rhBMP4, whereby sb4 increases BRE-luc expression 2-fold at 0.4 ng/ml of rhBMP4[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Sb4 関連抗体

    Western Blot Analysis[1]

    Cell Line: Primary mouse kidney epithelial cells (PRECs)
    Concentration: 0.05 μM-1 μM
    Incubation Time: 24 hours
    Result: Increased p-SMAD-1/5/9 abundance in PRECs.

    Western Blot Analysis[1]

    Cell Line: BRE-Luc cells
    Concentration: 1 μM
    Incubation Time: 0min, 5 mins, 15 mins, 30 mins, 45 mins, 60 mins
    Result: Acted to stabilize p-SMAD-1/5/9 to enhance the transcriptional response.
    分子量

    320.20

    分子式

    C14H10BrNOS
    CAS 番号
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    BrC1=CC=C(CSC2=NC3=CC=CC=C3O2)C=C1
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 100 mg/mL (312.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.1230 mL 15.6152 mL 31.2305 mL
    5 mM 0.6246 mL 3.1230 mL 6.2461 mL
    10 mM 0.3123 mL 1.5615 mL 3.1230 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

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    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (7.81 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: 2.5 mg/mL (7.81 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.98%

    COA (245 KB) HNMR (247 KB) RP-HPLC (168 KB) LCMS (382 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.1230 mL 15.6152 mL 31.2305 mL 78.0762 mL
    5 mM 0.6246 mL 3.1230 mL 6.2461 mL 15.6152 mL
    10 mM 0.3123 mL 1.5615 mL 3.1230 mL 7.8076 mL
    15 mM 0.2082 mL 1.0410 mL 2.0820 mL 5.2051 mL
    20 mM 0.1562 mL 0.7808 mL 1.5615 mL 3.9038 mL
    25 mM 0.1249 mL 0.6246 mL 1.2492 mL 3.1230 mL
    30 mM 0.1041 mL 0.5205 mL 1.0410 mL 2.6025 mL
    40 mM 0.0781 mL 0.3904 mL 0.7808 mL 1.9519 mL
    50 mM 0.0625 mL 0.3123 mL 0.6246 mL 1.5615 mL
    60 mM 0.0521 mL 0.2603 mL 0.5205 mL 1.3013 mL
    80 mM 0.0390 mL 0.1952 mL 0.3904 mL 0.9760 mL
    100 mM 0.0312 mL 0.1562 mL 0.3123 mL 0.7808 mL
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    BMP signaling agonist sb4 Related Classifications

    • Molarity Calculator

    • Dilution Calculator

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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    BMP signaling agonist sb4100874-08-6BMP signaling agonist sb 4BMP signaling agonist sb-4TGF-β ReceptorTransforming growth factor beta receptorsbmpSMADagonistPRECBRE-luccellInhibitorinhibitorinhibit

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    製品名:
    BMP signaling agonist sb4
    製品番号:
    HY-124697
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