2. Immunology/Inflammation TGF-beta/Smad Stem Cell/Wnt
  3. AP-1 PKA
  4. BRP

BRP  (Synonyms: BRINP2-related peptide)

Cat. No.: HY-P10927 Purity: 95.15%
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Handling Instructions Technical Support

BRP is a 12-peptide derived from BRINP2 that can cross the blood-brain barrier. BRP induces the central activation of FOS in neuronal cells via the cAMP-PKA-CREB signaling pathway. BRP exerts anorectic and anti-obesity effects without triggering nausea or aversive responses. The action of BRP is independent of the leptin, GLP-1 receptor and melanocortin 4 receptor pathways. BRP is applicable to obesity-related research.

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Custom Peptide Synthesis

BRP

BRP 화학구조

사이즈 가격 재고 수량
1 mg 해외재고보유
5 mg 해외재고보유
10 mg 해외재고보유
25 mg 해외재고보유
50 mg   견적 받기  
100 mg   견적 받기  

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 1 Customer Validation

Other Forms of BRP:

BRP Related Antibodies

Top Publications Citing Use of Products

View All AP-1 Isoform Specific Products:

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c-Fos FosB Fra1/FOSL1 Fra2/FOSL2 c-Jun

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PKA PKAR PKACα

  • Biological Activity

  • 순도&문서

  • References

  • 고객리뷰

제품 설명

BRP is a 12-peptide derived from BRINP2 that can cross the blood-brain barrier. BRP induces the central activation of FOS in neuronal cells via the cAMP-PKA-CREB signaling pathway. BRP exerts anorectic and anti-obesity effects without triggering nausea or aversive responses. The action of BRP is independent of the leptin, GLP-1 receptor and melanocortin 4 receptor pathways. BRP is applicable to obesity-related research[1].

In Vitro

BRP (50-100 μg/mL; 0-24 h) induces potent, sustained, and non-desensitizing Fos expression in NS-1 rat neuronal cells, with EC50 values of 33.7 μM at 24 h and 37 μM at 1 h[1].
BRP (50-100 μg/ml; 0-24 h) induces potent, sustained and non-desensitizing Fos expression in mouse Neuro2a neuronal cells, with EC50 values of 33 μM at 24 h and 27 μM at 1 h, and activates the CREB and ERK1/2 signaling pathways[1].
BRP (100 μg/mL; 0-40 min) increases intracellular cAMP levels in Neuro2a mouse neuronal cells[1].
BRP (100 μg/mL; 15 min) induces phosphorylation of CREB at Ser133, phosphorylation of ERK1/2, and phosphorylation of PKA substrates in Neuro2a neuronal cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

BRP Related Antibodies
In Vivo

BRP (0.5-20 mg/kg; i.p., i.n., i.c.v.; single dose) dose-dependently inhibits food intake in lean male and female C57BL/6J mice when administered via intraperitoneal, intranasal or intracerebroventricular routes[1].
BRP (5 mg/kg; i.p.; daily; 14 days) reverses obesity and improves glucose homeostasis in diet-induced obese male C57BL/6J mice by reducing food intake and fat mass[1].
BRP (2 mg/kg; i.m.; single dose) suppresses feeding behavior in male Yucatan miniature pigs without inducing anxiety-like behavior[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6J (8-week-old male; 8-week-old female)[1]
Dosage: 0.5-20 mg/kg (i.p., single dose); 10 mg/kg (i.n., single dose); 0.5 mg/kg (i.c.v., single dose)
Administration: i.p. (single dose); i.n. (single dose); i.c.v. (single dose, infused into lateral ventricle over ~30s)
Result: Potently and immediately suppressed food intake for up to 3 hours in lean male mice at 5 mg/kg i.p..
Almost completely suppressed food intake over 3 hours in lean male mice at 20 mg/kg i.p..
Did not lower food intake in lean male mice at 0.5 mg/kg i.p..
Suppressed food intake over 3 hours in lean female mice at 5 mg/kg i.p..
Suppressed food intake over 3 hours in lean male mice at 10 mg/kg i.p..
Suppressed food intake over 3 hours in lean male mice at 10 mg/kg i.n..
Suppressed food intake over 3 hours in lean male mice at 0.5 mg/kg i.c.v..
Animal Model: C57BL/6J (6-week diet-induced obese male)[1]
Dosage: 1-5 mg/kg
Administration: i.p. (daily; 14 days)
Result: Reduced accumulated food intake and body weight.
Improved glucose tolerance and insulin tolerance.
Reduced inguinal white adipose tissue mass, reduced brown adipose tissue mass, reduced liver mass, reduced adipocyte size in inguinal white adipose tissue, reduced ectopic hepatic lipid accumulation, and reduced fat mass without affecting lean mass.
분자량

1540.88

화학식

C68H117N25O14S
Appearance

Solid

Color

White to off-white

Sequence

Thr-His-Arg-Ile-Leu-Arg-Arg-Leu-Phe-Asn-Leu-Cys-NH2
Sequence Shortening

THRILRRLFNLC-NH2
선적

Room temperature in continental US; may vary elsewhere.
보관

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

용액&용해도
In Vitro: 

H2O : ≥ 100 mg/mL (64.90 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.6490 mL 3.2449 mL 6.4898 mL
5 mM 0.1298 mL 0.6490 mL 1.2980 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • 몰농도 계산기

  • 농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
순도&문서
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 0.6490 mL 3.2449 mL 6.4898 mL 16.2245 mL
5 mM 0.1298 mL 0.6490 mL 1.2980 mL 3.2449 mL
10 mM 0.0649 mL 0.3245 mL 0.6490 mL 1.6224 mL
15 mM 0.0433 mL 0.2163 mL 0.4327 mL 1.0816 mL
20 mM 0.0324 mL 0.1622 mL 0.3245 mL 0.8112 mL
25 mM 0.0260 mL 0.1298 mL 0.2596 mL 0.6490 mL
30 mM 0.0216 mL 0.1082 mL 0.2163 mL 0.5408 mL
40 mM 0.0162 mL 0.0811 mL 0.1622 mL 0.4056 mL
50 mM 0.0130 mL 0.0649 mL 0.1298 mL 0.3245 mL
60 mM 0.0108 mL 0.0541 mL 0.1082 mL 0.2704 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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BRP Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BRPBRINP2-related peptideAP-1PKAActivator Protein 1Protein kinase AmiceNS-1 rat neuronal cellsINS1 832/13 rat insulinoma cellsNeuro2a mouse neuronal cellspigshypothalamic nucleiBRINP2FOSblood-brain barriercAMP-PKA-CREB signalling pathwayInhibitorinhibitorinhibit

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상품명:
BRP
Cat. No.:
HY-P10927
수량:
MCE Japan Authorized Agent:

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