2. TGF-beta/Smad Stem Cell/Wnt Immunology/Inflammation
  3. PKA AP-1
  4. BRP TFA

BRP (BRINP2-related peptide) TFA is a 12-peptide derived from BRINP2 that can cross the blood-brain barrier. BRP TFA induces the central activation of FOS in neuronal cells via the cAMP-PKA-CREB signaling pathway. BRP TFA exerts anorectic and anti-obesity effects without triggering nausea or aversive responses. The action of BRP TFA is independent of the leptin, GLP-1 receptor and melanocortin 4 receptor pathways. BRP TFA is applicable to obesity-related research.

For research use only. We do not sell to patients.

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BRP TFA

BRP TFA Chemical Structure

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Based on 1 publication(s) in Google Scholar

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Description

BRP (BRINP2-related peptide) TFA is a 12-peptide derived from BRINP2 that can cross the blood-brain barrier. BRP TFA induces the central activation of FOS in neuronal cells via the cAMP-PKA-CREB signaling pathway. BRP TFA exerts anorectic and anti-obesity effects without triggering nausea or aversive responses. The action of BRP TFA is independent of the leptin, GLP-1 receptor and melanocortin 4 receptor pathways. BRP TFA is applicable to obesity-related research[1].

In Vitro

BRP (50-100 μg/mL; 0-24 h) TFA induces potent, sustained, and non-desensitizing Fos expression in NS-1 rat neuronal cells, with EC50 values of 33.7 μM at 24 h and 37 μM at 1 h[1].
BRP (50-100 μg/ml; 0-24 h) TFA induces potent, sustained and non-desensitizing Fos expression in mouse Neuro2a neuronal cells, with EC50 values of 33 μM at 24 h and 27 μM at 1 h, and activates the CREB and ERK1/2 signaling pathways[1].
BRP (100 μg/mL; 0-40 min) TFA increases intracellular cAMP levels in Neuro2a mouse neuronal cells[1].
BRP (100 μg/mL; 15 min) TFA induces phosphorylation of CREB at Ser133, phosphorylation of ERK1/2, and phosphorylation of PKA substrates in Neuro2a neuronal cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

BRP TFA Related Antibodies
In Vivo

BRP (0.5-20 mg/kg; i.p., i.n., i.c.v.; single dose) TFA dose-dependently inhibits food intake in lean male and female C57BL/6J mice when administered via intraperitoneal, intranasal or intracerebroventricular routes[1].
BRP (5 mg/kg; i.p.; daily; 14 days) TFA reverses obesity and improves glucose homeostasis in diet-induced obese male C57BL/6J mice by reducing food intake and fat mass[1].
BRP (2 mg/kg; i.m.; single dose) TFA suppresses feeding behavior in male Yucatan miniature pigs without inducing anxiety-like behavior[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6J (8-week-old male; 8-week-old female)[1]
Dosage: 0.5-20 mg/kg (i.p., single dose); 10 mg/kg (i.n., single dose); 0.5 mg/kg (i.c.v., single dose)
Administration: i.p. (single dose); i.n. (single dose); i.c.v. (single dose, infused into lateral ventricle over ~30s)
Result: Potently and immediately suppressed food intake for up to 3 hours in lean male mice at 5 mg/kg i.p..
Almost completely suppressed food intake over 3 hours in lean male mice at 20 mg/kg i.p..
Did not lower food intake in lean male mice at 0.5 mg/kg i.p..
Suppressed food intake over 3 hours in lean female mice at 5 mg/kg i.p..
Suppressed food intake over 3 hours in lean male mice at 10 mg/kg i.p..
Suppressed food intake over 3 hours in lean male mice at 10 mg/kg i.n..
Suppressed food intake over 3 hours in lean male mice at 0.5 mg/kg i.c.v..
Animal Model: C57BL/6J (6-week diet-induced obese male)[1]
Dosage: 1-5 mg/kg
Administration: i.p. (daily; 14 days)
Result: Reduced accumulated food intake and body weight.
Improved glucose tolerance and insulin tolerance.
Reduced inguinal white adipose tissue mass, reduced brown adipose tissue mass, reduced liver mass, reduced adipocyte size in inguinal white adipose tissue, reduced ectopic hepatic lipid accumulation, and reduced fat mass without affecting lean mass.
Molecular Weight

1540.88 (free base)

Formula

C68H117N25O14S.xC2HF3O2
Appearance

Solid

Sequence

Thr-His-Arg-Ile-Leu-Arg-Arg-Leu-Phe-Asn-Leu-Cys-NH2
Sequence Shortening

THRILRRLFNLC-NH2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL

*"≥" means soluble, but saturation unknown.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C1V1 = C2V2

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Purity & Documentation
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BRP TFA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BRPBRINP2-related peptidePKAAP-1Protein kinase AActivator Protein 1miceNS-1 rat neuronal cellsINS1 832/13 rat insulinoma cellsNeuro2a mouse neuronal cellspigshypothalamic nucleiBRINP2FOSblood-brain barriercAMP-PKA-CREB signalling pathwayInhibitorinhibitorinhibit

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