2. 疾患モデリングル製品 Immunology/Inflammation
  3. Immunology and Inflammatory Disease Models Toll-like Receptor (TLR)
  4. Arthritis Models Myocarditis Models
  5. Complete Freund's adjuvant (CFA)

フロイント 完全 アジュバント  (Synonyms: Complete Freund's adjuvant (CFA))

製品番号: HY-153808
COA 取扱説明書 Technical Support

Complete Freund's adjuvant (CFA) is an immunoadjuvant emulsified with antigen by its discoverer Jules T. Freund to enhance an animal's immune response to an antigen. Complete Freund's adjuvant (CFA) is also an inducer of the Th1 immune response and a ligand of TLRs. Complete Freund's adjuvant (CFA) contains heat-killed inactive tuberculosis bacilli and consists of a paraffin oil-in-water emulsion. Complete Freund's adjuvant (CFA) stimulates a strong and durable immune response and can be used to induce persistent inflammatory pain models in mice, experimental autoimmune myocarditis (EAM) models, and more. Incomplete Freund's adjuvant (IFA) (HY-153808A) is another type of Freund's Adjuvant that stimulates a weaker immune response.

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Complete Freund's adjuvant (CFA)

フロイント 完全 アジュバント 構造式

CAS 番号 : 9007-81-2

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10 mL * 2 USD 50 在庫あり
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10 mL * 5 USD 105 在庫あり

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Based on 15 publication(s) in Google Scholar

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Toll-like Receptor (TLR) アイソフォーム固有の製品をすべて表示:

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TLR3 TLR8 TLR9 TLR2 TLR4 TLR7 TLR1 TLR6

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製品説明

Complete Freund's adjuvant (CFA) is an immunoadjuvant emulsified with antigen by its discoverer Jules T. Freund to enhance an animal's immune response to an antigen. Complete Freund's adjuvant (CFA) is also an inducer of the Th1 immune response and a ligand of TLRs. Complete Freund's adjuvant (CFA) contains heat-killed inactive tuberculosis bacilli and consists of a paraffin oil-in-water emulsion. Complete Freund's adjuvant (CFA) stimulates a strong and durable immune response and can be used to induce persistent inflammatory pain models in mice, experimental autoimmune myocarditis (EAM) models, and more. Incomplete Freund's adjuvant (IFA) (HY-153808A) is another type of Freund's Adjuvant that stimulates a weaker immune response[1][2].

体外実験

Complete Freund's adjuvant (CFA) contains numerous microbial TLR ligands (such as TLRs 2, 4, and 9), is a potent inducer of a Th1-type immune response[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

フロイント 完全 アジュバント 関連抗体
体内実験

Note:
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

Complete Freund's adjuvant (CFA) induces experimental autoimmune myocarditis and neuropathic pain, with a relatively low plasma clearance rate (22%)[1][2][3][4].

Induction of experimental autoimmune myocarditis (EAM)[1]
Background
Complete Freund's adjuvant (CFA) induces myocarditis by activating the immune system and enhancing the antigen-specific immune response.
Specific Modeling Methods
Mice: A/J, BALB/c, and IL6KO BALB/c • male • 6-8-week-old
Administration: 5mg/ml • s.c. • twice
Note
(1) On day 0, all mice additionally received 500 ng of pertussis toxin (i.p.)
(2) On days 0 and 7, mice received subcutaneous injections peptide emulsified in CFA supplemented with 5 mg/mL of Mycobacterium tuberculosis, strain H37Ra.
Modeling Indicators
Histological analysis: Myocarditis severity was assessed by histology and relative heart weight.
Molecular changes: Test serum levels of total IgG, IgG1, IgG2a, IgG2b, IL-1b and IL6.
Correlated Product(s): Incomplete Freund's adjuvant (IFA) (HY-153808A); Complete Freund's adjuvant (CFA, 1 mg/ml) (HY-153808B)

Induction of Neuropathic Pain[2][3]
Background
Complete Freund's adjuvant (CFA) releases inflammatory mediators TNF-α and IL-6 in body which can activate peripheral nerve endings and act on pain nerve endings, resulting in neuropathic pain[2][3].
Specific Modeling Methods
Mice: C57BL/6J • male • 8-week-old
Administration: 20 μl • s.c. • once
Note
(1) Inject Complete Freund’s Adjuvant (CFA) without dilution into the plantar area of the hind paw.
(2) Tests PWT and PWL for both hind paws corresponding to before and 24, 48, 72 h after CFA injection.
Modeling Indicators
Behavior Observation: Detect paw withdrawal threshold (PWT) and paw withdrawal latency (PWL).
Correlated Product(s): Galantamine (HY-76299)
Opposite Product(s): Mecamylamine (HY-B1395A)

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: A/J, BALB/c, and IL6KO BALB/c male mice[1]
Dosage:
Administration: Subcutaneous Injectionon (sc); injected at days 0 and 7. EAM severity was analyzed by histology on day 21 (Repeated administration of CFA to animals can cause multiple injection-site effects, the most often observed are on site granulomas and pain)
Result: Increased the relative proportion of CD11b+F4/80−monocytes and CD11b+Gr1+ granulocytes.
Expanded monocytes in the heart and in the spleen and those monocytes in the spleen make less IL-10 as well as more IL-6.
CAS 番号
Appearance

Liquid

Color

Colorless to light yellow

SMILES

[Complete Freund's adjuvant (CFA)]
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

Store at 4°C, protect from light, do not freeze

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, do not freeze)
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COA (240 KB)

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参考文献
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Complete Freund's adjuvant (CFA) Related Classifications

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    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Complete Freund's adjuvant (CFA)9007-81-2Toll-like Receptor (TLR)immunologic adjuvantimmunityinflammationimmunization-induced animal modelscollagen-induced arthritisCIAexperimental autoimmune encephalomyelitisEAEexperimental autoimmune thyroiditisEATexperimental autoimmune uveitisEAUexperimental autoimmune myocarditisEAMInhibitorinhibitorinhibit

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Complete Freund's adjuvant (CFA)
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