Dopamine (Synonyms: ASL279 free base)

Name: Dopamine
Brand: MedChemExpress
SKU: HY-B0451
Rating: 5.0 (21 reviews)

Cat. No.: HY-B0451 Purity: 98.56%: Data Sheet
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Dopamine is a catecholamine neurotransmitter that is produced in the substantia nigra, ventral tegmental area, and hypothalamus of the brain. Dopamine plays several important roles in the brain and body. Dopamine acts through D2 dopamine receptors to induce endocytosis of VEGFR2, which is critical for promoting angiogenesis.

For research use only. We do not sell to patients.

CAS No. : 51-61-6

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 21 publication(s) in Google Scholar

Other Forms of Dopamine:

Dopamine hydrochloride In-stock

21 Publications Citing Use of MCE Dopamine

Histological Imaging/Staining

: Dopamine purchased from MedChemExpress. Usage Cited in: bioRxiv. 2023 Oct 13.; Representative images of fluorescence of TR-D3 in the anterior and posterior brain sections, brainstem, the cervical, thoracic and lumbar spinal cord, and liver in control and in the presence of dopamine (DA).

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Biological Activity
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Description

Dopamine is a catecholamine neurotransmitter that is produced in the substantia nigra, ventral tegmental area, and hypothalamus of the brain. Dopamine plays several important roles in the brain and body^[1]. Dopamine acts through D2 dopamine receptors to induce endocytosis of VEGFR2, which is critical for promoting angiogenesis^[1].

IC₅₀ & Target

Microbial Metabolite

D2 Dopamine Receptor

Cellular Effect

Cell Line	Type	Value	Description	References
CHO-K1	EC₅₀	0.69 1 Compound: Dopamine	Agonist activity at D2 receptor (unknown origin) expressed in CHOK1 cells co-expressing G-alpha 15 by fluo-4 dye based FLIPR assay Agonist activity at D2 receptor (unknown origin) expressed in CHOK1 cells co-expressing G-alpha 15 by fluo-4 dye based FLIPR assay	[PMID: 27487565]
AtT20	EC₅₀	8.53 1 Compound: DA, dopamine	Agonist activity at human cloned dopamine D3 receptor expressed in AtT-20 cells assessed as stimulation of [35S]GTP-gamma-S binding Agonist activity at human cloned dopamine D3 receptor expressed in AtT-20 cells assessed as stimulation of [35S]GTP-gamma-S binding	[PMID: 18072730]
AtT20	EC₅₀	8.53 1 Compound: dopamine	Agonist activity at human dopamine D3 receptor expressed in mouse AtT-20 cells assessed as stimulation of [35S]GTPgamma binding Agonist activity at human dopamine D3 receptor expressed in mouse AtT-20 cells assessed as stimulation of [35S]GTPgamma binding	[PMID: 20038106]
HEK293	EC₅₀	0.8 1 Compound: 1; DA	Agonist activity at D2R (unknown origin) expressed in HEK293 cells assessed as effect on cAMP accumulation incubated for 10 mins by Gi-cAMP Glosensor assay Agonist activity at D2R (unknown origin) expressed in HEK293 cells assessed as effect on cAMP accumulation incubated for 10 mins by Gi-cAMP Glosensor assay	[PMID: 31098001]
CHO	EC₅₀	10.6 1 Compound: Dopamine	Agonist activity at human dopamine D3 receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding Agonist activity at human dopamine D3 receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding	[PMID: 24471976]
HEK293	EC₅₀	1.1 1 Compound: dopamine	Agonist activity at rat D2 receptor in HEK293 cells coexpressing G-alpha-qo5 by FLIPR Agonist activity at rat D2 receptor in HEK293 cells coexpressing G-alpha-qo5 by FLIPR	[PMID: 17149874]
HEK293	EC₅₀	1.1 1 Compound: DA	Agonist activity at RLuc8-fused Go-coupled human D3R expressed in HEK293 cells untagged beta1 and mVenus-tagged gamma2 measured after 5 mins in presence of coelenterazine H by BRET assay Agonist activity at RLuc8-fused Go-coupled human D3R expressed in HEK293 cells untagged beta1 and mVenus-tagged gamma2 measured after 5 mins in presence of coelenterazine H by BRET assay	[PMID: 32342685]
CHO	EC₅₀	183 1 Compound: DA	Agonist activity at human D2 receptor expressed in CHO cells by [35S]GTPgammaS binding assay Agonist activity at human D2 receptor expressed in CHO cells by [35S]GTPgammaS binding assay	[PMID: 26555041]
HEK293	EC₅₀	1.1 1 Compound: Dopamine	In vitro effective concentration tested on HEK293 cells co-transfected with rat Dopamine receptor D2 using FLIPR assay In vitro effective concentration tested on HEK293 cells co-transfected with rat Dopamine receptor D2 using FLIPR assay	[PMID: 15239663]
CHO	EC₅₀	209 1 Compound: DA, dopamine	Agonist activity at human cloned dopamine D2L receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding Agonist activity at human cloned dopamine D2L receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding	[PMID: 18072730]
CHO-K1	EC₅₀	1.8 1 Compound: Dopamine	Agonist activity at dopamine D3 receptor (unknown origin) expressed in CHOK1 cells coexpressing Galpha15 by FLIPR assay Agonist activity at dopamine D3 receptor (unknown origin) expressed in CHOK1 cells coexpressing Galpha15 by FLIPR assay	[PMID: 23675993]
CHO	EC₅₀	209 1 Compound: Dopamine	Agonist activity at human dopamine D2L receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding Agonist activity at human dopamine D2L receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding	[PMID: 18410082]
HEK293	EC₅₀	1.8 1 Compound: 1; DA	Agonist activity at D4R (unknown origin) expressed in HEK293 cells assessed as effect on cAMP accumulation incubated for 10 mins by Gi-cAMP Glosensor assay Agonist activity at D4R (unknown origin) expressed in HEK293 cells assessed as effect on cAMP accumulation incubated for 10 mins by Gi-cAMP Glosensor assay	[PMID: 31098001]
CHO	EC₅₀	209 1 Compound: Dopamine	Activity at human dopamine D2L receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding Activity at human dopamine D2L receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding	[PMID: 19427222]
HEK-293T	EC₅₀	1.8 1 Compound: DA	Agonist activity at human D3 receptor transfected in HEK293T cells by BRET based G0 activation assay Agonist activity at human D3 receptor transfected in HEK293T cells by BRET based G0 activation assay	[PMID: 27035329]
CHO	EC₅₀	209 1 Compound: Dopamine	Agonist activity at human dopamine D2 receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding Agonist activity at human dopamine D2 receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding	[PMID: 20605099]
CHO	EC₅₀	209 1 Compound: dopamine	Agonist activity at human dopamine D2L receptor expressed in CHO cells assessed as stimulation of [35S]GTPgamma binding Agonist activity at human dopamine D2L receptor expressed in CHO cells assessed as stimulation of [35S]GTPgamma binding	[PMID: 20038106]
HEK293	EC₅₀	2 1 Compound: 1; DA	Agonist activity at D5R (unknown origin) expressed in HEK293 cells assessed as effect on cAMP accumulation incubated for 10 mins by Gs-cAMP Glosensor assay Agonist activity at D5R (unknown origin) expressed in HEK293 cells assessed as effect on cAMP accumulation incubated for 10 mins by Gs-cAMP Glosensor assay	[PMID: 31098001]
HEK293	EC₅₀	2.2 1 Compound: dopamine	Agonist activity at human D4.4 receptor in HEK293 cells coexpressing G-alpha-qo5 by FLIPR Agonist activity at human D4.4 receptor in HEK293 cells coexpressing G-alpha-qo5 by FLIPR	[PMID: 17149874]
CHO	EC₅₀	209 1 Compound: dopamine	Agonist activity at human dopamine D2 receptor expressed in CHO cells by [35S]GTPgammaS binding assay Agonist activity at human dopamine D2 receptor expressed in CHO cells by [35S]GTPgammaS binding assay	[PMID: 20146482]
CHO	EC₅₀	218 1 Compound: Dopamine	Agonist activity at human dopamine D2 receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding Agonist activity at human dopamine D2 receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding	[PMID: 24471976]
HEK293	EC₅₀	2.2 1 Compound: Dopamine	In vitro effective concentration tested on HEK293 cells co-transfected with human Dopamine receptor D4 using FLIPR assay In vitro effective concentration tested on HEK293 cells co-transfected with human Dopamine receptor D4 using FLIPR assay	[PMID: 15239663]
HEK293	EC₅₀	2.3 1 Compound: DA	Agonist activity at RLuc8-fused human D3R expressed in HEK293 cells co-expressing N-terminal Venus-tagged beta-arrestin2 and GRK3 assessed as induction of beta-arrestin2 recruitment measured after 5 mins in presence of coelenterazine H by BRET assay Agonist activity at RLuc8-fused human D3R expressed in HEK293 cells co-expressing N-terminal Venus-tagged beta-arrestin2 and GRK3 assessed as induction of beta-arrestin2 recruitment measured after 5 mins in presence of coelenterazine H by BRET assay	[PMID: 32342685]
CHO	EC₅₀	227 1 Compound: DA	Agonist activity at human cloned dopamine D2 receptor expressed in CHO cells by [35S]GTPgammaS binding assay Agonist activity at human cloned dopamine D2 receptor expressed in CHO cells by [35S]GTPgammaS binding assay	[PMID: 22642365]
HEK293	EC₅₀	2.4 1 Compound: dopamine	Agonist activity at rat D4 receptor in HEK293 cells coexpressing G-alpha-qo5 by FLIPR Agonist activity at rat D4 receptor in HEK293 cells coexpressing G-alpha-qo5 by FLIPR	[PMID: 17149874]
CHO	EC₅₀	227 1 Compound: DA	Agonist activity at human dopamine D2L receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding after 60 mins by scintillation counting analysis Agonist activity at human dopamine D2L receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding after 60 mins by scintillation counting analysis	[PMID: 23623679]
HEK293	EC₅₀	2.4 1 Compound: Dopamine	In vitro effective concentration tested on HEK293 cells co-transfected with rat Dopamine receptor D4 using FLIPR assay In vitro effective concentration tested on HEK293 cells co-transfected with rat Dopamine receptor D4 using FLIPR assay	[PMID: 15239663]
CHO	EC₅₀	240 1 Compound: Dopamine	Agonist activity at human cloned dopamine D2 receptor expressed in CHO cells by [35S]GTP gammaS binding assay Agonist activity at human cloned dopamine D2 receptor expressed in CHO cells by [35S]GTP gammaS binding assay	[PMID: 23275802]
HEK293	EC₅₀	2.7 1 Compound: dopamine	Agonist activity at ferret D4 receptor in HEK293 cells coexpressing G-alpha-qo5 by FLIPR Agonist activity at ferret D4 receptor in HEK293 cells coexpressing G-alpha-qo5 by FLIPR	[PMID: 17149874]
CHO	EC₅₀	35 1 Compound: dopamine	Agonist activity at human recombinant dopamine D1 receptor expressed in CHO cells assessed as stimulation of cAMP production Agonist activity at human recombinant dopamine D1 receptor expressed in CHO cells assessed as stimulation of cAMP production	[PMID: 18313303]
CHO	EC₅₀	36 1 Compound: Dopamine	Agonist activity at human dopamine D2 receptor expressed in CHO cells assessed as increase of forskolin-induced cAMP production after 20 mins Agonist activity at human dopamine D2 receptor expressed in CHO cells assessed as increase of forskolin-induced cAMP production after 20 mins	[PMID: 24296012]
HEK293	EC₅₀	2.7 1 Compound: Dopamine	In vitro effective concentration tested on HEK293 cells co-transfected with ferret Dopamine receptor D4 using FLIPR assay In vitro effective concentration tested on HEK293 cells co-transfected with ferret Dopamine receptor D4 using FLIPR assay	[PMID: 15239663]
HEK293	EC₅₀	3.5 1 Compound: DA	Agonist activity at human D3R expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP production measured after 5 mins in presence of beta-adrenergic blocker propranolol and coelenterazine H by CAMYEL BRET assay Agonist activity at human D3R expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP production measured after 5 mins in presence of beta-adrenergic blocker propranolol and coelenterazine H by CAMYEL BRET assay	[PMID: 32342685]
CHO	EC₅₀	376 1 Compound: Dopamine	Agonist activity at human D2 dopamine receptor expressed in CHO cells by [35S]GTPgammaS binding assay Agonist activity at human D2 dopamine receptor expressed in CHO cells by [35S]GTPgammaS binding assay	[PMID: 27591013]
CHO	EC₅₀	4.76 1 Compound: Dopamine	Agonist activity at human dopamine D3 receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding Agonist activity at human dopamine D3 receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding	[PMID: 20605099]
CHO	EC₅₀	39 1 Compound: Dopamine	Agonist activity at human recombinant dopamine D1 receptor expressed in CHO cells assessed as cAMP production after 20 mins Agonist activity at human recombinant dopamine D1 receptor expressed in CHO cells assessed as cAMP production after 20 mins	[PMID: 24296012]
CHO	EC₅₀	4.76 1 Compound: dopamine	Agonist activity at human dopamine D3 receptor expressed in CHO cells by [35S]GTPgammaS binding assay Agonist activity at human dopamine D3 receptor expressed in CHO cells by [35S]GTPgammaS binding assay	[PMID: 20146482]
CHO	EC₅₀	4.76 1 Compound: Dopamine	Agonist activity at human dopamine D3 receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding Agonist activity at human dopamine D3 receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding	[PMID: 20605099]
CHO	EC₅₀	4.76 1 Compound: dopamine	Agonist activity at human dopamine D3 receptor expressed in CHO cells by [35S]GTPgammaS binding assay Agonist activity at human dopamine D3 receptor expressed in CHO cells by [35S]GTPgammaS binding assay	[PMID: 20146482]
HEK293	EC₅₀	5 1 Compound: dopamine	Activity at rat dopamine D2L receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP production Activity at rat dopamine D2L receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP production	[PMID: 17154515]
CHO	EC₅₀	43 1 Compound: dopamine	Agonist activity at human dopamine D2 receptor expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP production Agonist activity at human dopamine D2 receptor expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP production	[PMID: 18313303]
HEK-293T	EC₅₀	5 1 Compound: DA	Agonist activity at human D2 receptor transfected in HEK293T cells by BRET based G0 activation assay Agonist activity at human D2 receptor transfected in HEK293T cells by BRET based G0 activation assay	[PMID: 27035329]
CHO	EC₅₀	5.34 1 Compound: DA	Agonist activity at human D3 receptor expressed in CHO cells by [35S]GTPgammaS binding assay Agonist activity at human D3 receptor expressed in CHO cells by [35S]GTPgammaS binding assay	[PMID: 26555041]
CHO	EC₅₀	5.34 1 Compound: DA	Agonist activity at human D3 receptor expressed in CHO cells by [35S]GTPgammaS binding assay Agonist activity at human D3 receptor expressed in CHO cells by [35S]GTPgammaS binding assay	[PMID: 26555041]
CHO	EC₅₀	5.8 1 Compound: Dopamine	Agonist activity at human cloned dopamine D3 receptor expressed in CHO cells by [35S]GTP gammaS binding assay Agonist activity at human cloned dopamine D3 receptor expressed in CHO cells by [35S]GTP gammaS binding assay	[PMID: 23275802]
CHO	EC₅₀	5.8 1 Compound: Dopamine	Agonist activity at human cloned dopamine D3 receptor expressed in CHO cells by [35S]GTP gammaS binding assay Agonist activity at human cloned dopamine D3 receptor expressed in CHO cells by [35S]GTP gammaS binding assay	[PMID: 23275802]
HEK293	IC₅₀	6.63 1 Compound: Dopamine	Inhibition of [3H]norepinephrine uptake in HEK cells expressing human NET Inhibition of [3H]norepinephrine uptake in HEK cells expressing human NET	[PMID: 14592523]
CHO	EC₅₀	7.26 1 Compound: Dopamine	Agonist activity at human D3 dopamine receptor expressed in CHO cells by [35S]GTPgammaS binding assay Agonist activity at human D3 dopamine receptor expressed in CHO cells by [35S]GTPgammaS binding assay	[PMID: 27591013]
CHO	EC₅₀	7.64 1 Compound: DA	Agonist activity at human dopamine D3 receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding after 60 mins by scintillation counting analysis Agonist activity at human dopamine D3 receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding after 60 mins by scintillation counting analysis	[PMID: 23623679]
CHO-K1	EC₅₀	6.7 1 Compound: Dopamine	Agonist activity at dopamine D2 receptor (unknown origin) expressed in CHOK1 cells coexpressing Galpha15 by FLIPR assay Agonist activity at dopamine D2 receptor (unknown origin) expressed in CHOK1 cells coexpressing Galpha15 by FLIPR assay	[PMID: 23675993]
CHO	EC₅₀	8.57 1 Compound: DA	Agonist activity at human cloned dopamine D3 receptor expressed in CHO cells by [35S]GTPgammaS binding assay Agonist activity at human cloned dopamine D3 receptor expressed in CHO cells by [35S]GTPgammaS binding assay	[PMID: 22642365]
CHO	EC₅₀	7.26 1 Compound: Dopamine	Agonist activity at human D3 dopamine receptor expressed in CHO cells by [35S]GTPgammaS binding assay Agonist activity at human D3 dopamine receptor expressed in CHO cells by [35S]GTPgammaS binding assay	[PMID: 27591013]
CHO	EC₅₀	7.64 1 Compound: DA	Agonist activity at human dopamine D3 receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding after 60 mins by scintillation counting analysis Agonist activity at human dopamine D3 receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding after 60 mins by scintillation counting analysis	[PMID: 23623679]
HEK293	EC₅₀	8 1 Compound: dopamine	Agonist activity at ferret D2 receptor in HEK293 cells coexpressing Galphaqo5 by FLIPR Agonist activity at ferret D2 receptor in HEK293 cells coexpressing Galphaqo5 by FLIPR	[PMID: 17149874]
HEK293	EC₅₀	8 1 Compound: Dopamine	In vitro effective concentration tested on HEK293 cells co-transfected with ferret Dopamine receptor D2 using FLIPR assay In vitro effective concentration tested on HEK293 cells co-transfected with ferret Dopamine receptor D2 using FLIPR assay	[PMID: 15239663]
AtT20	EC₅₀	8.53 1 Compound: DA, dopamine	Agonist activity at human cloned dopamine D3 receptor expressed in AtT-20 cells assessed as stimulation of [35S]GTP-gamma-S binding Agonist activity at human cloned dopamine D3 receptor expressed in AtT-20 cells assessed as stimulation of [35S]GTP-gamma-S binding	[PMID: 18072730]
AtT20	EC₅₀	8.53 1 Compound: dopamine	Agonist activity at human dopamine D3 receptor expressed in mouse AtT-20 cells assessed as stimulation of [35S]GTPgamma binding Agonist activity at human dopamine D3 receptor expressed in mouse AtT-20 cells assessed as stimulation of [35S]GTPgamma binding	[PMID: 20038106]
CHO	EC₅₀	8.57 1 Compound: DA	Agonist activity at human cloned dopamine D3 receptor expressed in CHO cells by [35S]GTPgammaS binding assay Agonist activity at human cloned dopamine D3 receptor expressed in CHO cells by [35S]GTPgammaS binding assay	[PMID: 22642365]
HEK293	EC₅₀	9.3 1 Compound: Dopamine	Agonist activity at human dopamine D3 receptor transiently expressed in HEK293 cells co-expressing Galphao1 after 30 mins by [35S]GTPgammaS binding assay Agonist activity at human dopamine D3 receptor transiently expressed in HEK293 cells co-expressing Galphao1 after 30 mins by [35S]GTPgammaS binding assay	[PMID: 24831693]
HEK293	EC₅₀	10 1 Compound: dopamine	Activity at human dopamine D4.4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP production Activity at human dopamine D4.4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP production	[PMID: 17154515]
CHO	EC₅₀	10.6 1 Compound: Dopamine	Agonist activity at human dopamine D3 receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding Agonist activity at human dopamine D3 receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding	[PMID: 24471976]
HEK293	IC₅₀	>30 3 Compound: Dopamine	Inhibition of [3H]5-HT uptake in HEK cells expressing human SERT Inhibition of [3H]5-HT uptake in HEK cells expressing human SERT	[PMID: 14592523]
HEK293	EC₅₀	18 1 Compound: dopamine	Agonist activity at human D2 receptor in HEK293 cells coexpressing G-alpha-qo5 by FLIPR Agonist activity at human D2 receptor in HEK293 cells coexpressing G-alpha-qo5 by FLIPR	[PMID: 17149874]
HEK293	EC₅₀	18 1 Compound: Dopamine	In vitro effective concentration tested on HEK293 cells co-transfected with human Dopamine receptor D2 using FLIPR assay In vitro effective concentration tested on HEK293 cells co-transfected with human Dopamine receptor D2 using FLIPR assay	[PMID: 15239663]
HRPE	IC₅₀	62 3 Compound: Dopamine	TP_TRANSPORTER: inhibition of MPP+ uptake (MPP+: 1 uM) in OCT3-expressing HRPE cells TP_TRANSPORTER: inhibition of MPP+ uptake (MPP+: 1 uM) in OCT3-expressing HRPE cells	[PMID: 9830022]
HRPE	IC₅₀	2300 3 Compound: Dopamine	TP_TRANSPORTER: inhibition of TEA uptake (TEA: 20 uM) in OCT2-expressing HRPE cells TP_TRANSPORTER: inhibition of TEA uptake (TEA: 20 uM) in OCT2-expressing HRPE cells	[PMID: 9830022]
HRPE	IC₅₀	384 3 Compound: Dopamine	TP_TRANSPORTER: inhibition of TEA uptake (TEA: 20 uM) in OCT3-expressing HRPE cells TP_TRANSPORTER: inhibition of TEA uptake (TEA: 20 uM) in OCT3-expressing HRPE cells	[PMID: 9830022]
HEK293	EC₅₀	33 1 Compound: DA	Agonist activity at RLuc8-fused human D3R Y365A mutant expressed in HEK293 cells co-expressing N-terminal Venus-tagged beta-arrestin2 and GRK3 assessed as induction of beta-arrestin2 recruitment measured after 5 mins in presence of coelenterazine H by BRE Agonist activity at RLuc8-fused human D3R Y365A mutant expressed in HEK293 cells co-expressing N-terminal Venus-tagged beta-arrestin2 and GRK3 assessed as induction of beta-arrestin2 recruitment measured after 5 mins in presence of coelenterazine H by BRE	[PMID: 32342685]
CHO	EC₅₀	35 1 Compound: dopamine	Agonist activity at human recombinant dopamine D1 receptor expressed in CHO cells assessed as stimulation of cAMP production Agonist activity at human recombinant dopamine D1 receptor expressed in CHO cells assessed as stimulation of cAMP production	[PMID: 18313303]
CHO	EC₅₀	36 1 Compound: Dopamine	Agonist activity at human dopamine D2 receptor expressed in CHO cells assessed as increase of forskolin-induced cAMP production after 20 mins Agonist activity at human dopamine D2 receptor expressed in CHO cells assessed as increase of forskolin-induced cAMP production after 20 mins	[PMID: 24296012]
CHO	EC₅₀	39 1 Compound: Dopamine	Agonist activity at human recombinant dopamine D1 receptor expressed in CHO cells assessed as cAMP production after 20 mins Agonist activity at human recombinant dopamine D1 receptor expressed in CHO cells assessed as cAMP production after 20 mins	[PMID: 24296012]
CHO	EC₅₀	43 1 Compound: dopamine	Agonist activity at human dopamine D2 receptor expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP production Agonist activity at human dopamine D2 receptor expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP production	[PMID: 18313303]
HEK293	EC₅₀	44 1 Compound: 1; DA	Agonist activity at wild type human D1R expressed in HEK293 cells assessed as effect on cAMP accumulation incubated for 10 mins by Gs-cAMP Glosensor assay Agonist activity at wild type human D1R expressed in HEK293 cells assessed as effect on cAMP accumulation incubated for 10 mins by Gs-cAMP Glosensor assay	[PMID: 31098001]
HEK293	EC₅₀	55 1 Compound: DA	Agonist activity at RLuc8-fused human D3R Y198A mutant expressed in HEK293 cells co-expressing N-terminal Venus-tagged beta-arrestin2 and GRK3 assessed as induction of beta-arrestin2 recruitment measured after 5 mins in presence of coelenterazine H by BRE Agonist activity at RLuc8-fused human D3R Y198A mutant expressed in HEK293 cells co-expressing N-terminal Venus-tagged beta-arrestin2 and GRK3 assessed as induction of beta-arrestin2 recruitment measured after 5 mins in presence of coelenterazine H by BRE	[PMID: 32342685]
COS-7	IC₅₀	111 1 Compound: Dopamine	Displacement of [3H]nemonapride from Rattus norvegicus (rat) wild type dopamine D3 receptor transfected in african green monkey COS7 cells after 1 hr by beta scintillation counting Displacement of [3H]nemonapride from Rattus norvegicus (rat) wild type dopamine D3 receptor transfected in african green monkey COS7 cells after 1 hr by beta scintillation counting	10.1007/s00044-004-0006-x
HEK293	EC₅₀	120 1 Compound: dopamine	Activity at human dopamine D1 receptor expressed in HEK293 cells assessed as stimulation of cAMP production Activity at human dopamine D1 receptor expressed in HEK293 cells assessed as stimulation of cAMP production	[PMID: 17154515]
MRC5	IC₅₀	68.9 3 Compound: 35	Cytotoxicity against human MRC5 cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay Cytotoxicity against human MRC5 cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay	[PMID: 27155468]
COS-7	IC₅₀	124 1 Compound: Dopamine	Displacement of [3H]nemonapride from Rattus norvegicus (rat) chimeric dopamine D3 trunk/D3 tail receptor transfected in african green monkey COS7 cells after 1 hr by beta scintillation counting Displacement of [3H]nemonapride from Rattus norvegicus (rat) chimeric dopamine D3 trunk/D3 tail receptor transfected in african green monkey COS7 cells after 1 hr by beta scintillation counting	10.1007/s00044-004-0006-x
CHO-K1	EC₅₀	0.69 1 Compound: Dopamine	Agonist activity at D2 receptor (unknown origin) expressed in CHOK1 cells co-expressing G-alpha 15 by fluo-4 dye based FLIPR assay Agonist activity at D2 receptor (unknown origin) expressed in CHOK1 cells co-expressing G-alpha 15 by fluo-4 dye based FLIPR assay	[PMID: 27487565]
HEK-293T	EC₅₀	160 1 Compound: Dopamine	Partial agonist activity at human D2SR expressed in HEK293T cells co-expressing (EA)beta-arrestin2 and GRK2 assessed as induction of beta-arrestin2 recruitment after 5 hrs by chemiluminescence assay Partial agonist activity at human D2SR expressed in HEK293T cells co-expressing (EA)beta-arrestin2 and GRK2 assessed as induction of beta-arrestin2 recruitment after 5 hrs by chemiluminescence assay	[PMID: 28489379]
CHO	EC₅₀	183 1 Compound: DA	Agonist activity at human D2 receptor expressed in CHO cells by [35S]GTPgammaS binding assay Agonist activity at human D2 receptor expressed in CHO cells by [35S]GTPgammaS binding assay	[PMID: 26555041]
CHO-K1	EC₅₀	1.8 1 Compound: Dopamine	Agonist activity at dopamine D3 receptor (unknown origin) expressed in CHOK1 cells coexpressing Galpha15 by FLIPR assay Agonist activity at dopamine D3 receptor (unknown origin) expressed in CHOK1 cells coexpressing Galpha15 by FLIPR assay	[PMID: 23675993]
CHO-K1	EC₅₀	1425 1 Compound: Dopamine	Agonist activity at rat dopamine D2short receptor expressed in CHO-K1 cells assessed as stimulation of [35S]GTPgammaS binding by liquid scintillation counting Agonist activity at rat dopamine D2short receptor expressed in CHO-K1 cells assessed as stimulation of [35S]GTPgammaS binding by liquid scintillation counting	[PMID: 19454369]
HEK-293T	EC₅₀	200 1 Compound: Dopamine	Agonist activity at human D2S receptor expressed in HEK293T cell membranes coexpressing Galphao1 assessed as induction of nucleotide exchange preincubated for 30 mins followed by addition of [35S]GTPgammaS measured after 30 mins by [35S]GTPgammaS binding Agonist activity at human D2S receptor expressed in HEK293T cell membranes coexpressing Galphao1 assessed as induction of nucleotide exchange preincubated for 30 mins followed by addition of [35S]GTPgammaS measured after 30 mins by [35S]GTPgammaS binding	[PMID: 28489379]
CHO	EC₅₀	209 1 Compound: Dopamine	Activity at human dopamine D2L receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding Activity at human dopamine D2L receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding	[PMID: 19427222]
CHO-K1	EC₅₀	6.7 1 Compound: Dopamine	Agonist activity at dopamine D2 receptor (unknown origin) expressed in CHOK1 cells coexpressing Galpha15 by FLIPR assay Agonist activity at dopamine D2 receptor (unknown origin) expressed in CHOK1 cells coexpressing Galpha15 by FLIPR assay	[PMID: 23675993]
CHO	EC₅₀	209 1 Compound: DA, dopamine	Agonist activity at human cloned dopamine D2L receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding Agonist activity at human cloned dopamine D2L receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding	[PMID: 18072730]
CHO	EC₅₀	209 1 Compound: Dopamine	Agonist activity at human dopamine D2 receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding Agonist activity at human dopamine D2 receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding	[PMID: 20605099]
COS-7	IC₅₀	111 1 Compound: Dopamine	Displacement of [3H]nemonapride from Rattus norvegicus (rat) wild type dopamine D3 receptor transfected in african green monkey COS7 cells after 1 hr by beta scintillation counting Displacement of [3H]nemonapride from Rattus norvegicus (rat) wild type dopamine D3 receptor transfected in african green monkey COS7 cells after 1 hr by beta scintillation counting	10.1007/s00044-004-0006-x
CHO	EC₅₀	209 1 Compound: dopamine	Agonist activity at human dopamine D2 receptor expressed in CHO cells by [35S]GTPgammaS binding assay Agonist activity at human dopamine D2 receptor expressed in CHO cells by [35S]GTPgammaS binding assay	[PMID: 20146482]
COS-7	IC₅₀	124 1 Compound: Dopamine	Displacement of [3H]nemonapride from Rattus norvegicus (rat) chimeric dopamine D3 trunk/D3 tail receptor transfected in african green monkey COS7 cells after 1 hr by beta scintillation counting Displacement of [3H]nemonapride from Rattus norvegicus (rat) chimeric dopamine D3 trunk/D3 tail receptor transfected in african green monkey COS7 cells after 1 hr by beta scintillation counting	10.1007/s00044-004-0006-x
CHO	EC₅₀	209 1 Compound: dopamine	Agonist activity at human dopamine D2L receptor expressed in CHO cells assessed as stimulation of [35S]GTPgamma binding Agonist activity at human dopamine D2L receptor expressed in CHO cells assessed as stimulation of [35S]GTPgamma binding	[PMID: 20038106]
COS-7	IC₅₀	3140 1 Compound: Dopamine	Displacement of [3H]nemonapride from Rattus norvegicus (rat) wild type dopamine D2 receptor transfected in african green monkey COS7 cells after 1 hr by beta scintillation counting Displacement of [3H]nemonapride from Rattus norvegicus (rat) wild type dopamine D2 receptor transfected in african green monkey COS7 cells after 1 hr by beta scintillation counting	10.1007/s00044-004-0006-x
CHO	EC₅₀	209 1 Compound: Dopamine	Agonist activity at human dopamine D2L receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding Agonist activity at human dopamine D2L receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding	[PMID: 18410082]
COS-7	IC₅₀	3260 1 Compound: Dopamine	Displacement of [3H]nemonapride from Rattus norvegicus (rat) chimeric dopamine D2 trunk/D2 tail receptor transfected in african green monkey COS7 cells after 1 hr by beta scintillation counting Displacement of [3H]nemonapride from Rattus norvegicus (rat) chimeric dopamine D2 trunk/D2 tail receptor transfected in african green monkey COS7 cells after 1 hr by beta scintillation counting	10.1007/s00044-004-0006-x
COS-7	IC₅₀	483 1 Compound: Dopamine	Displacement of [3H]nemonapride from Rattus norvegicus (rat) chimeric dopamine D3 trunk/D2 tail receptor transfected in african green monkey COS7 cells after 1 hr by beta scintillation counting Displacement of [3H]nemonapride from Rattus norvegicus (rat) chimeric dopamine D3 trunk/D2 tail receptor transfected in african green monkey COS7 cells after 1 hr by beta scintillation counting	10.1007/s00044-004-0006-x
CHO	EC₅₀	218 1 Compound: Dopamine	Agonist activity at human dopamine D2 receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding Agonist activity at human dopamine D2 receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding	[PMID: 24471976]
COS-7	IC₅₀	582 1 Compound: Dopamine	Displacement of [3H]nemonapride from Rattus norvegicus (rat) chimeric dopamine D2 trunk/D3 tail receptor transfected in african green monkey COS7 cells after 1 hr by beta scintillation counting Displacement of [3H]nemonapride from Rattus norvegicus (rat) chimeric dopamine D2 trunk/D3 tail receptor transfected in african green monkey COS7 cells after 1 hr by beta scintillation counting	10.1007/s00044-004-0006-x
CHO	EC₅₀	227 1 Compound: DA	Agonist activity at human cloned dopamine D2 receptor expressed in CHO cells by [35S]GTPgammaS binding assay Agonist activity at human cloned dopamine D2 receptor expressed in CHO cells by [35S]GTPgammaS binding assay	[PMID: 22642365]
CHO	EC₅₀	227 1 Compound: DA	Agonist activity at human dopamine D2L receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding after 60 mins by scintillation counting analysis Agonist activity at human dopamine D2L receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding after 60 mins by scintillation counting analysis	[PMID: 23623679]
CHO	EC₅₀	240 1 Compound: Dopamine	Agonist activity at human cloned dopamine D2 receptor expressed in CHO cells by [35S]GTP gammaS binding assay Agonist activity at human cloned dopamine D2 receptor expressed in CHO cells by [35S]GTP gammaS binding assay	[PMID: 23275802]
CHO	EC₅₀	376 1 Compound: Dopamine	Agonist activity at human D2 dopamine receptor expressed in CHO cells by [35S]GTPgammaS binding assay Agonist activity at human D2 dopamine receptor expressed in CHO cells by [35S]GTPgammaS binding assay	[PMID: 27591013]
HEK-293T	EC₅₀	390 1 Compound: Dopamine	Partial agonist activity at human D2SR expressed in HEK293T cells co-expressing (EA)beta-arrestin2 assessed as induction of beta-arrestin2 recruitment after 5 hrs by chemiluminescence assay Partial agonist activity at human D2SR expressed in HEK293T cells co-expressing (EA)beta-arrestin2 assessed as induction of beta-arrestin2 recruitment after 5 hrs by chemiluminescence assay	[PMID: 28489379]
HEK293	IC₅₀	460 1 Compound: Dopamine	Inhibition of [3H]dopamine uptake in HEK cells expressing human DAT Inhibition of [3H]dopamine uptake in HEK cells expressing human DAT	[PMID: 14592523]
HEK-293T	EC₅₀	1.8 1 Compound: DA	Agonist activity at human D3 receptor transfected in HEK293T cells by BRET based G0 activation assay Agonist activity at human D3 receptor transfected in HEK293T cells by BRET based G0 activation assay	[PMID: 27035329]
HEK293	EC₅₀	470 1 Compound: Dopamine	Agonist activity at human dopamine D2 short receptor transiently expressed in HEK293 cells co-expressing Galphai2 after 30 mins by [35S]GTPgammaS binding assay Agonist activity at human dopamine D2 short receptor transiently expressed in HEK293 cells co-expressing Galphai2 after 30 mins by [35S]GTPgammaS binding assay	[PMID: 24831693]
COS-7	IC₅₀	483 1 Compound: Dopamine	Displacement of [3H]nemonapride from Rattus norvegicus (rat) chimeric dopamine D3 trunk/D2 tail receptor transfected in african green monkey COS7 cells after 1 hr by beta scintillation counting Displacement of [3H]nemonapride from Rattus norvegicus (rat) chimeric dopamine D3 trunk/D2 tail receptor transfected in african green monkey COS7 cells after 1 hr by beta scintillation counting	10.1007/s00044-004-0006-x
HEK-293T	EC₅₀	160 1 Compound: Dopamine	Partial agonist activity at human D2SR expressed in HEK293T cells co-expressing (EA)beta-arrestin2 and GRK2 assessed as induction of beta-arrestin2 recruitment after 5 hrs by chemiluminescence assay Partial agonist activity at human D2SR expressed in HEK293T cells co-expressing (EA)beta-arrestin2 and GRK2 assessed as induction of beta-arrestin2 recruitment after 5 hrs by chemiluminescence assay	[PMID: 28489379]
HEK-293T	EC₅₀	200 1 Compound: Dopamine	Agonist activity at human D2S receptor expressed in HEK293T cell membranes coexpressing Galphao1 assessed as induction of nucleotide exchange preincubated for 30 mins followed by addition of [35S]GTPgammaS measured after 30 mins by [35S]GTPgammaS binding Agonist activity at human D2S receptor expressed in HEK293T cell membranes coexpressing Galphao1 assessed as induction of nucleotide exchange preincubated for 30 mins followed by addition of [35S]GTPgammaS measured after 30 mins by [35S]GTPgammaS binding	[PMID: 28489379]
COS-7	IC₅₀	582 1 Compound: Dopamine	Displacement of [3H]nemonapride from Rattus norvegicus (rat) chimeric dopamine D2 trunk/D3 tail receptor transfected in african green monkey COS7 cells after 1 hr by beta scintillation counting Displacement of [3H]nemonapride from Rattus norvegicus (rat) chimeric dopamine D2 trunk/D3 tail receptor transfected in african green monkey COS7 cells after 1 hr by beta scintillation counting	10.1007/s00044-004-0006-x
RAW264.7	EC₅₀	18 3 Compound: 11	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production measured after 24 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production measured after 24 hrs by Griess assay	[PMID: 29517238]
HEK-293T	EC₅₀	390 1 Compound: Dopamine	Partial agonist activity at human D2SR expressed in HEK293T cells co-expressing (EA)beta-arrestin2 assessed as induction of beta-arrestin2 recruitment after 5 hrs by chemiluminescence assay Partial agonist activity at human D2SR expressed in HEK293T cells co-expressing (EA)beta-arrestin2 assessed as induction of beta-arrestin2 recruitment after 5 hrs by chemiluminescence assay	[PMID: 28489379]
HEK-293T	EC₅₀	5 1 Compound: DA	Agonist activity at human D2 receptor transfected in HEK293T cells by BRET based G0 activation assay Agonist activity at human D2 receptor transfected in HEK293T cells by BRET based G0 activation assay	[PMID: 27035329]
HEK293	EC₅₀	820 1 Compound: DA	Agonist activity at RLuc8-fused human D3R Y198A/Y365A double mutant expressed in HEK293 cells co-expressing N-terminal Venus-tagged beta-arrestin2 and GRK3 assessed as induction of beta-arrestin2 recruitment measured after 5 mins in presence of coelentera Agonist activity at RLuc8-fused human D3R Y198A/Y365A double mutant expressed in HEK293 cells co-expressing N-terminal Venus-tagged beta-arrestin2 and GRK3 assessed as induction of beta-arrestin2 recruitment measured after 5 mins in presence of coelentera	[PMID: 32342685]
Sf21	IC₅₀	>1 2 Compound: Dopamine	Inhibition of Sprague-Dawley rat Bsep expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake Inhibition of Sprague-Dawley rat Bsep expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake	[PMID: 21965623]
HEK293	EC₅₀	1400 1 Compound: 1; DA	Agonist activity at wild type human D1R expressed in HEK293 cells assessed as effect on beta-arrestin2 recruitment by PRESTO-Tango beta-arrestin2 recruitment assay Agonist activity at wild type human D1R expressed in HEK293 cells assessed as effect on beta-arrestin2 recruitment by PRESTO-Tango beta-arrestin2 recruitment assay	[PMID: 31098001]
CHO-K1	EC₅₀	1425 1 Compound: Dopamine	Agonist activity at rat dopamine D2short receptor expressed in CHO-K1 cells assessed as stimulation of [35S]GTPgammaS binding by liquid scintillation counting Agonist activity at rat dopamine D2short receptor expressed in CHO-K1 cells assessed as stimulation of [35S]GTPgammaS binding by liquid scintillation counting	[PMID: 19454369]
COS-7	IC₅₀	3140 1 Compound: Dopamine	Displacement of [3H]nemonapride from Rattus norvegicus (rat) wild type dopamine D2 receptor transfected in african green monkey COS7 cells after 1 hr by beta scintillation counting Displacement of [3H]nemonapride from Rattus norvegicus (rat) wild type dopamine D2 receptor transfected in african green monkey COS7 cells after 1 hr by beta scintillation counting	10.1007/s00044-004-0006-x
COS-7	IC₅₀	3260 1 Compound: Dopamine	Displacement of [3H]nemonapride from Rattus norvegicus (rat) chimeric dopamine D2 trunk/D2 tail receptor transfected in african green monkey COS7 cells after 1 hr by beta scintillation counting Displacement of [3H]nemonapride from Rattus norvegicus (rat) chimeric dopamine D2 trunk/D2 tail receptor transfected in african green monkey COS7 cells after 1 hr by beta scintillation counting	10.1007/s00044-004-0006-x
HEK293	EC₅₀	0.8 1 Compound: 1; DA	Agonist activity at D2R (unknown origin) expressed in HEK293 cells assessed as effect on cAMP accumulation incubated for 10 mins by Gi-cAMP Glosensor assay Agonist activity at D2R (unknown origin) expressed in HEK293 cells assessed as effect on cAMP accumulation incubated for 10 mins by Gi-cAMP Glosensor assay	[PMID: 31098001]
HEK293	EC₅₀	1.1 1 Compound: DA	Agonist activity at RLuc8-fused Go-coupled human D3R expressed in HEK293 cells untagged beta1 and mVenus-tagged gamma2 measured after 5 mins in presence of coelenterazine H by BRET assay Agonist activity at RLuc8-fused Go-coupled human D3R expressed in HEK293 cells untagged beta1 and mVenus-tagged gamma2 measured after 5 mins in presence of coelenterazine H by BRET assay	[PMID: 32342685]
HEK293	EC₅₀	1.1 1 Compound: Dopamine	In vitro effective concentration tested on HEK293 cells co-transfected with rat Dopamine receptor D2 using FLIPR assay In vitro effective concentration tested on HEK293 cells co-transfected with rat Dopamine receptor D2 using FLIPR assay	[PMID: 15239663]
HEK293	EC₅₀	1.1 1 Compound: dopamine	Agonist activity at rat D2 receptor in HEK293 cells coexpressing G-alpha-qo5 by FLIPR Agonist activity at rat D2 receptor in HEK293 cells coexpressing G-alpha-qo5 by FLIPR	[PMID: 17149874]
HEK293	EC₅₀	1.8 1 Compound: 1; DA	Agonist activity at D4R (unknown origin) expressed in HEK293 cells assessed as effect on cAMP accumulation incubated for 10 mins by Gi-cAMP Glosensor assay Agonist activity at D4R (unknown origin) expressed in HEK293 cells assessed as effect on cAMP accumulation incubated for 10 mins by Gi-cAMP Glosensor assay	[PMID: 31098001]
HEK293	EC₅₀	10 1 Compound: dopamine	Activity at human dopamine D4.4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP production Activity at human dopamine D4.4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP production	[PMID: 17154515]
HEK293	EC₅₀	120 1 Compound: dopamine	Activity at human dopamine D1 receptor expressed in HEK293 cells assessed as stimulation of cAMP production Activity at human dopamine D1 receptor expressed in HEK293 cells assessed as stimulation of cAMP production	[PMID: 17154515]
HEK293	EC₅₀	1400 1 Compound: 1; DA	Agonist activity at wild type human D1R expressed in HEK293 cells assessed as effect on beta-arrestin2 recruitment by PRESTO-Tango beta-arrestin2 recruitment assay Agonist activity at wild type human D1R expressed in HEK293 cells assessed as effect on beta-arrestin2 recruitment by PRESTO-Tango beta-arrestin2 recruitment assay	[PMID: 31098001]
HEK293	EC₅₀	18 1 Compound: Dopamine	In vitro effective concentration tested on HEK293 cells co-transfected with human Dopamine receptor D2 using FLIPR assay In vitro effective concentration tested on HEK293 cells co-transfected with human Dopamine receptor D2 using FLIPR assay	[PMID: 15239663]
HEK293	EC₅₀	18 1 Compound: dopamine	Agonist activity at human D2 receptor in HEK293 cells coexpressing G-alpha-qo5 by FLIPR Agonist activity at human D2 receptor in HEK293 cells coexpressing G-alpha-qo5 by FLIPR	[PMID: 17149874]
HEK293	EC₅₀	2 1 Compound: 1; DA	Agonist activity at D5R (unknown origin) expressed in HEK293 cells assessed as effect on cAMP accumulation incubated for 10 mins by Gs-cAMP Glosensor assay Agonist activity at D5R (unknown origin) expressed in HEK293 cells assessed as effect on cAMP accumulation incubated for 10 mins by Gs-cAMP Glosensor assay	[PMID: 31098001]
HEK293	EC₅₀	2.2 1 Compound: Dopamine	In vitro effective concentration tested on HEK293 cells co-transfected with human Dopamine receptor D4 using FLIPR assay In vitro effective concentration tested on HEK293 cells co-transfected with human Dopamine receptor D4 using FLIPR assay	[PMID: 15239663]
HEK293	EC₅₀	2.2 1 Compound: dopamine	Agonist activity at human D4.4 receptor in HEK293 cells coexpressing G-alpha-qo5 by FLIPR Agonist activity at human D4.4 receptor in HEK293 cells coexpressing G-alpha-qo5 by FLIPR	[PMID: 17149874]
HEK293	EC₅₀	2.3 1 Compound: DA	Agonist activity at RLuc8-fused human D3R expressed in HEK293 cells co-expressing N-terminal Venus-tagged beta-arrestin2 and GRK3 assessed as induction of beta-arrestin2 recruitment measured after 5 mins in presence of coelenterazine H by BRET assay Agonist activity at RLuc8-fused human D3R expressed in HEK293 cells co-expressing N-terminal Venus-tagged beta-arrestin2 and GRK3 assessed as induction of beta-arrestin2 recruitment measured after 5 mins in presence of coelenterazine H by BRET assay	[PMID: 32342685]
HEK293	EC₅₀	2.4 1 Compound: Dopamine	In vitro effective concentration tested on HEK293 cells co-transfected with rat Dopamine receptor D4 using FLIPR assay In vitro effective concentration tested on HEK293 cells co-transfected with rat Dopamine receptor D4 using FLIPR assay	[PMID: 15239663]
HEK293	EC₅₀	2.4 1 Compound: dopamine	Agonist activity at rat D4 receptor in HEK293 cells coexpressing G-alpha-qo5 by FLIPR Agonist activity at rat D4 receptor in HEK293 cells coexpressing G-alpha-qo5 by FLIPR	[PMID: 17149874]
HEK293	EC₅₀	2.7 1 Compound: Dopamine	In vitro effective concentration tested on HEK293 cells co-transfected with ferret Dopamine receptor D4 using FLIPR assay In vitro effective concentration tested on HEK293 cells co-transfected with ferret Dopamine receptor D4 using FLIPR assay	[PMID: 15239663]
HEK293	EC₅₀	2.7 1 Compound: dopamine	Agonist activity at ferret D4 receptor in HEK293 cells coexpressing G-alpha-qo5 by FLIPR Agonist activity at ferret D4 receptor in HEK293 cells coexpressing G-alpha-qo5 by FLIPR	[PMID: 17149874]
HEK293	EC₅₀	3.5 1 Compound: DA	Agonist activity at human D3R expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP production measured after 5 mins in presence of beta-adrenergic blocker propranolol and coelenterazine H by CAMYEL BRET assay Agonist activity at human D3R expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP production measured after 5 mins in presence of beta-adrenergic blocker propranolol and coelenterazine H by CAMYEL BRET assay	[PMID: 32342685]
HEK293	EC₅₀	33 1 Compound: DA	Agonist activity at RLuc8-fused human D3R Y365A mutant expressed in HEK293 cells co-expressing N-terminal Venus-tagged beta-arrestin2 and GRK3 assessed as induction of beta-arrestin2 recruitment measured after 5 mins in presence of coelenterazine H by BRE Agonist activity at RLuc8-fused human D3R Y365A mutant expressed in HEK293 cells co-expressing N-terminal Venus-tagged beta-arrestin2 and GRK3 assessed as induction of beta-arrestin2 recruitment measured after 5 mins in presence of coelenterazine H by BRE	[PMID: 32342685]
HEK293	EC₅₀	44 1 Compound: 1; DA	Agonist activity at wild type human D1R expressed in HEK293 cells assessed as effect on cAMP accumulation incubated for 10 mins by Gs-cAMP Glosensor assay Agonist activity at wild type human D1R expressed in HEK293 cells assessed as effect on cAMP accumulation incubated for 10 mins by Gs-cAMP Glosensor assay	[PMID: 31098001]
HEK293	EC₅₀	470 1 Compound: Dopamine	Agonist activity at human dopamine D2 short receptor transiently expressed in HEK293 cells co-expressing Galphai2 after 30 mins by [35S]GTPgammaS binding assay Agonist activity at human dopamine D2 short receptor transiently expressed in HEK293 cells co-expressing Galphai2 after 30 mins by [35S]GTPgammaS binding assay	[PMID: 24831693]
HEK293	EC₅₀	5 1 Compound: dopamine	Activity at rat dopamine D2L receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP production Activity at rat dopamine D2L receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP production	[PMID: 17154515]
HEK293	EC₅₀	55 1 Compound: DA	Agonist activity at RLuc8-fused human D3R Y198A mutant expressed in HEK293 cells co-expressing N-terminal Venus-tagged beta-arrestin2 and GRK3 assessed as induction of beta-arrestin2 recruitment measured after 5 mins in presence of coelenterazine H by BRE Agonist activity at RLuc8-fused human D3R Y198A mutant expressed in HEK293 cells co-expressing N-terminal Venus-tagged beta-arrestin2 and GRK3 assessed as induction of beta-arrestin2 recruitment measured after 5 mins in presence of coelenterazine H by BRE	[PMID: 32342685]
HEK293	EC₅₀	8 1 Compound: Dopamine	In vitro effective concentration tested on HEK293 cells co-transfected with ferret Dopamine receptor D2 using FLIPR assay In vitro effective concentration tested on HEK293 cells co-transfected with ferret Dopamine receptor D2 using FLIPR assay	[PMID: 15239663]
HEK293	EC₅₀	8 1 Compound: dopamine	Agonist activity at ferret D2 receptor in HEK293 cells coexpressing Galphaqo5 by FLIPR Agonist activity at ferret D2 receptor in HEK293 cells coexpressing Galphaqo5 by FLIPR	[PMID: 17149874]
HEK293	EC₅₀	820 1 Compound: DA	Agonist activity at RLuc8-fused human D3R Y198A/Y365A double mutant expressed in HEK293 cells co-expressing N-terminal Venus-tagged beta-arrestin2 and GRK3 assessed as induction of beta-arrestin2 recruitment measured after 5 mins in presence of coelentera Agonist activity at RLuc8-fused human D3R Y198A/Y365A double mutant expressed in HEK293 cells co-expressing N-terminal Venus-tagged beta-arrestin2 and GRK3 assessed as induction of beta-arrestin2 recruitment measured after 5 mins in presence of coelentera	[PMID: 32342685]
HEK293	EC₅₀	9.3 1 Compound: Dopamine	Agonist activity at human dopamine D3 receptor transiently expressed in HEK293 cells co-expressing Galphao1 after 30 mins by [35S]GTPgammaS binding assay Agonist activity at human dopamine D3 receptor transiently expressed in HEK293 cells co-expressing Galphao1 after 30 mins by [35S]GTPgammaS binding assay	[PMID: 24831693]
HEK293	IC₅₀	460 1 Compound: Dopamine	Inhibition of [3H]dopamine uptake in HEK cells expressing human DAT Inhibition of [3H]dopamine uptake in HEK cells expressing human DAT	[PMID: 14592523]
HEK293	IC₅₀	6.63 1 Compound: Dopamine	Inhibition of [3H]norepinephrine uptake in HEK cells expressing human NET Inhibition of [3H]norepinephrine uptake in HEK cells expressing human NET	[PMID: 14592523]
HEK293	IC₅₀	> 30000 1 Compound: Dopamine	Inhibition of [3H]5-HT uptake in HEK cells expressing human SERT Inhibition of [3H]5-HT uptake in HEK cells expressing human SERT	[PMID: 14592523]
HRPE	IC₅₀	2300 3 Compound: Dopamine	TP_TRANSPORTER: inhibition of TEA uptake (TEA: 20 uM) in OCT2-expressing HRPE cells TP_TRANSPORTER: inhibition of TEA uptake (TEA: 20 uM) in OCT2-expressing HRPE cells	[PMID: 9830022]
HRPE	IC₅₀	384 3 Compound: Dopamine	TP_TRANSPORTER: inhibition of TEA uptake (TEA: 20 uM) in OCT3-expressing HRPE cells TP_TRANSPORTER: inhibition of TEA uptake (TEA: 20 uM) in OCT3-expressing HRPE cells	[PMID: 9830022]
HRPE	IC₅₀	62 3 Compound: Dopamine	TP_TRANSPORTER: inhibition of MPP+ uptake (MPP+: 1 uM) in OCT3-expressing HRPE cells TP_TRANSPORTER: inhibition of MPP+ uptake (MPP+: 1 uM) in OCT3-expressing HRPE cells	[PMID: 9830022]
MRC5	IC₅₀	68.9 3 Compound: 35	Cytotoxicity against human MRC5 cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay Cytotoxicity against human MRC5 cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay	[PMID: 27155468]
RAW264.7	EC₅₀	18 3 Compound: 11	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production measured after 24 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production measured after 24 hrs by Griess assay	[PMID: 29517238]
Sf21	IC₅₀	> 1000 3 Compound: Dopamine	Inhibition of Sprague-Dawley rat Bsep expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake Inhibition of Sprague-Dawley rat Bsep expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake	[PMID: 21965623]
Sf21	IC₅₀	> 1000 3 Compound: Dopamine	Inhibition of human BSEP expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake Inhibition of human BSEP expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake	[PMID: 21965623]

In Vitro

Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF) strongly stimulates human umbilical vein endothelial cells (HUVECs) proliferation. The stimulatory effect of VPF/VEGF is abrogated by Dopamine (1 μM; 24hours)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[2]

Cell Line:	HUVECs
Concentration:	1 µM
Incubation Time:	24 hours
Result:	Inhibited VPF/VEGF-induced HUVEC proliferation specifically through its D2 receptor.

In Vivo

Dopamine (50 mg/kg; i.p. injection; days 1-7) strongly and selectively inhibits the vascular permeabilizing and angiogenic activities of VPF/VEGF^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Syngeneic C3Heb/FeJ mice with MOT ascites tumors^[2]
Dosage:	50 mg/kg
Administration:	I.p. injection; 7 days
Result:	Corresponding to ~5% of the median lethal dose (LD₅₀) in mice) beginning at 24 hours after tumor-cell injection and continuing daily for 7 days.

Clinical Trial

Molecular Weight

153.18

Formula

C₈H₁₁NO₂

CAS No.

51-61-6

Appearance

Solid

Color

Light brown to gray

SMILES

OC1=CC=C(CCN)C=C1O

Structure Classification

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (652.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.5283 mL	32.6413 mL	65.2827 mL
5 mM	1.3057 mL	6.5283 mL	13.0565 mL
10 mM	0.6528 mL	3.2641 mL	6.5283 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (16.32 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.56%

Select Batch:

Data Sheet (273 KB) SDS (393 KB)

COA (244 KB) HNMR (238 KB) RP-HPLC (206 KB) MS (68 KB)

Handling Instructions (2659 KB)

References

[1]. Juárez Olguín H, et al. The Role of Dopamine and Its Dysfunction as a Consequence of Oxidative Stress. Oxid Med Cell Longev. 2016;2016:9730467. [Content Brief]

[2]. Basu S, et al. The neurotransmitter dopamine inhibits angiogenesis induced by vascular permeability factor/vascular endothelial growth factor. Nat Med. 2001 May;7(5):569-74. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	6.5283 mL	32.6413 mL	65.2827 mL	163.2067 mL
	5 mM	1.3057 mL	6.5283 mL	13.0565 mL	32.6413 mL
	10 mM	0.6528 mL	3.2641 mL	6.5283 mL	16.3207 mL
	15 mM	0.4352 mL	2.1761 mL	4.3522 mL	10.8804 mL
	20 mM	0.3264 mL	1.6321 mL	3.2641 mL	8.1603 mL
	25 mM	0.2611 mL	1.3057 mL	2.6113 mL	6.5283 mL
	30 mM	0.2176 mL	1.0880 mL	2.1761 mL	5.4402 mL
	40 mM	0.1632 mL	0.8160 mL	1.6321 mL	4.0802 mL
	50 mM	0.1306 mL	0.6528 mL	1.3057 mL	3.2641 mL
	60 mM	0.1088 mL	0.5440 mL	1.0880 mL	2.7201 mL
	80 mM	0.0816 mL	0.4080 mL	0.8160 mL	2.0401 mL
	100 mM	0.0653 mL	0.3264 mL	0.6528 mL	1.6321 mL

Dopamine Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Dopamine51-61-6ASL279ASL 279ASL-279Dopamine ReceptorEndogenous MetaboliteFerroptosisHUVECsproliferationdopaminecatecholamineneurotransmitterInhibitorinhibitorinhibit

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Dopamine (Synonyms: ASL279 free base)

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