2. Neuronal Signaling GPCR/G Protein Metabolic Enzyme/Protease Apoptosis
  3. Dopamine Receptor Endogenous Metabolite Ferroptosis
  4. Dopamine hydrochloride

Dopamine hydrochloride  (Synonyms: ASL279)

Cat. No.: HY-B0451A Purity: 99.79%
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Dopamine hydrochloride (ASL279) is a catecholamine neurotransmitter that is produced in the substantia nigra, ventral tegmental area, and hypothalamus of the brain. Dopamine hydrochloride (ASL279) plays several important roles in the brain and body. Dopamine hydrochloride (ASL279) acts through D2 dopamine receptors to induce endocytosis of VEGFR2, which is critical for promoting angiogenesis.

For research use only. We do not sell to patients.

CAS No. : 62-31-7

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Based on 21 publication(s) in Google Scholar

Other Forms of Dopamine hydrochloride:

Dopamine hydrochloride Related Antibodies

Top Publications Citing Use of Products

    Dopamine hydrochloride purchased from MedChemExpress. Usage Cited in: bioRxiv. 2023 Oct 13.

    Representative images of fluorescence of TR-D3 in the anterior and posterior brain sections, brainstem, the cervical, thoracic and lumbar spinal cord, and liver in control and in the presence of dopamine (DA).

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    Dopamine Receptor D1 Receptor D2 Receptor D3 Receptor D4 Receptor D5 Receptor

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    Human Endogenous Metabolite Microbial Metabolite

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Dopamine hydrochloride (ASL279) is a catecholamine neurotransmitter that is produced in the substantia nigra, ventral tegmental area, and hypothalamus of the brain. Dopamine hydrochloride (ASL279) plays several important roles in the brain and body[1]. Dopamine hydrochloride (ASL279) acts through D2 dopamine receptors to induce endocytosis of VEGFR2, which is critical for promoting angiogenesis[1].

    IC50 & Target

    Microbial Metabolite

     

    D2 Dopamine Receptor

     

    Cellular Effect
    Cell Line Type Value Description References
    HEK293 EC50
    20 μM
    Compound: DA
    Inhibition of the Norepinephrine transporter (NET, SLC6A2) as assessed by GPCR-mediated changes in cell morphology using the impedance-based transporter activity through receptor activation (TRACT) assay in HEK-293 JumpIN-SLC6A2 cells (PubChem AID: 1745860)
    Inhibition of the Norepinephrine transporter (NET, SLC6A2) as assessed by GPCR-mediated changes in cell morphology using the impedance-based transporter activity through receptor activation (TRACT) assay in HEK-293 JumpIN-SLC6A2 cells (PubChem AID: 1745860)
    		[PMID: 34112854]
    HEK293 EC50
    37 nM
    Compound: 1, DA
    Agonist activity at human dopamine D1 receptor expressed in HEK293 cells assessed as cAMP accumulation after 15 mins
    Agonist activity at human dopamine D1 receptor expressed in HEK293 cells assessed as cAMP accumulation after 15 mins
    		[PMID: 21862338]
    HEK293 EC50
    46 μM
    Compound: dopamine
    Inhibition of the Dopamine transporter (DAT, SLC6A3) as assessed by GPCR-mediated changes in cell morphology using the impedance-based transporter activity through receptor activation (TRACT) assay in HEK-293 JumpIN-SLC6A3 cells (PubChem AID: 1745858)
    Inhibition of the Dopamine transporter (DAT, SLC6A3) as assessed by GPCR-mediated changes in cell morphology using the impedance-based transporter activity through receptor activation (TRACT) assay in HEK-293 JumpIN-SLC6A3 cells (PubChem AID: 1745858)
    		[PMID: 33446713]
    HEK293 IC50
    1 x 10-4 M
    Compound: dopamine
    Displacement of [3H]spiperone from low-affinity state of human dopamine D2L receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
    Displacement of [3H]spiperone from low-affinity state of human dopamine D2L receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
    		[PMID: 24325578]
    HEK293 IC50
    1 μM
    Compound: dopamine
    Displacement of [3H]spiperone from high-affinity state of human dopamine D2L receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
    Displacement of [3H]spiperone from high-affinity state of human dopamine D2L receptor transfected in HEK293 cells after 2 hrs by scintillation counting analysis
    		[PMID: 24325578]
    In Vitro

    Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF) strongly stimulates human umbilical vein endothelial cells (HUVECs) proliferation. The stimulatory effect of VPF/VEGF is abrogated by Dopamine (1 μM; 24hours)[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Dopamine hydrochloride Related Antibodies

    Cell Proliferation Assay[2]

    Cell Line: HUVECs
    Concentration: 1 µM
    Incubation Time: 24 hours
    Result: Inhibited VPF/VEGF-induced HUVEC proliferation specifically through its D2 receptor.
    In Vivo

    Dopamine (50 mg/kg; i.p. injection; days 1-7) strongly and selectively inhibits the vascular permeabilizing and angiogenic activities of VPF/VEGF[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Syngeneic C3Heb/FeJ mice with MOT ascites tumors[2]
    Dosage: 50 mg/kg
    Administration: I.p. injection; 7 days
    Result: Corresponding to ~5% of the median lethal dose (LD50) in mice) beginning at 24 hours after tumor-cell injection and continuing daily for 7 days.
    Molecular Weight

    189.64

    Formula

    C8H12ClNO2
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    OC1=CC=C(CCN)C=C1O.Cl
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light, stored under nitrogen

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

    Solvent & Solubility
    In Vitro: 

    H2O : ≥ 50 mg/mL (263.66 mM)

    DMSO : 50 mg/mL (263.66 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 5.2731 mL 26.3657 mL 52.7315 mL
    5 mM 1.0546 mL 5.2731 mL 10.5463 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.79%

    SDS (600 KB)

    COA (246 KB) HNMR (256 KB) RP-HPLC (205 KB) LCMS (95 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 5.2731 mL 26.3657 mL 52.7315 mL 131.8287 mL
    5 mM 1.0546 mL 5.2731 mL 10.5463 mL 26.3657 mL
    10 mM 0.5273 mL 2.6366 mL 5.2731 mL 13.1829 mL
    15 mM 0.3515 mL 1.7577 mL 3.5154 mL 8.7886 mL
    20 mM 0.2637 mL 1.3183 mL 2.6366 mL 6.5914 mL
    25 mM 0.2109 mL 1.0546 mL 2.1093 mL 5.2731 mL
    30 mM 0.1758 mL 0.8789 mL 1.7577 mL 4.3943 mL
    40 mM 0.1318 mL 0.6591 mL 1.3183 mL 3.2957 mL
    50 mM 0.1055 mL 0.5273 mL 1.0546 mL 2.6366 mL
    60 mM 0.0879 mL 0.4394 mL 0.8789 mL 2.1971 mL
    80 mM 0.0659 mL 0.3296 mL 0.6591 mL 1.6479 mL
    100 mM 0.0527 mL 0.2637 mL 0.5273 mL 1.3183 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Dopamine hydrochloride Related Classifications

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Dopamine62-31-7ASL279ASL 279ASL-279Dopamine ReceptorEndogenous MetaboliteFerroptosisHUVECsproliferationdopaminecatecholamineneurotransmitterInhibitorinhibitorinhibit

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