2. GPCR/G Protein Neuronal Signaling Metabolic Enzyme/Protease
  3. 5-HT Receptor COMT Endogenous Metabolite
  4. Serotonin hydrochloride

Serotonin hydrochloride  (Synonyms: 5-Hydroxytryptamine hydrochloride; 5-HT hydrochloride)

Cat. No.: HY-B1473 Purity: 99.97%
COA
SDS
Handling Instructions Technical Support

Serotonin (5-Hydroxytryptamine) hydrochloride is a monoamine neurotransmitter in the CNS and an endogenous 5-HT receptor agonist, with blood-brain barrier permeability. Serotonin hydrochloride is also a catechol O-methyltransferase (COMT) inhibitor with a Ki of 44 μM.

For research use only. We do not sell to patients.

CAS No. : 153-98-0

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
50 mg In-stock
100 mg In-stock
250 mg In-stock
500 mg   Get quote  
1 g   Get quote  

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Customer Review

Based on 31 publication(s) in Google Scholar

Other Forms of Serotonin hydrochloride:

Serotonin hydrochloride Related Antibodies

Top Publications Citing Use of Products
Cell Proliferation/Viability Assay
ELISA
Cell Migration/Invasion Assay
RT-PCR
WB

    Serotonin hydrochloride purchased from MedChemExpress. Usage Cited in: iScience. 2025 Nov 19;28(12):114120.  [Abstract]

    Scratch tests were used to observe the migration ability of MLE-12 cells treated with 5-HT (Serotonin, 10 μM) for 24 h.

    Serotonin hydrochloride purchased from MedChemExpress. Usage Cited in: iScience. 2025 Jun 18;28(7):112931.  [Abstract]

    SH-SY5Y cells were treated with 10 μM Serotonin (5-HT), then the intracellular 5-HT was detected through ELISA assay for 0, 60, 120 min.

    Serotonin hydrochloride purchased from MedChemExpress. Usage Cited in: Curr Res Food Sci. 2024 Apr 26:8:100754.  [Abstract]

    Dose-response and time course of cell viability after in vitro administration of 5-HT(Serotonin);

    Serotonin hydrochloride purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2021 Jul;9(7):e002383.  [Abstract]

    Relative IDO1 transcription levels detected by quantitative reverse transcription PCR (qRT-PCR) analysis in BT549 cells treated with dimethyl sulfoxide (DMSO), tetrahydrobiopterin (BH4, 10 µM), 5-HTP (500 µM), serotonin (10 µM), NAS (100 µM), and melatonin (1 µM) for 24 hours.

    Serotonin hydrochloride purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2021 Jul;9(7):e002383.  [Abstract]

    Relative IDO1 levels detected by western blot in BT549 cells treated with dimethyl sulfoxide (DMSO), tetrahydrobiopterin (BH4, 10 µM), 5-HTP (500 µM), serotonin (10 µM), NAS (100 µM), and melatonin (1 µM) for 24 hours.

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    Human Endogenous Metabolite Microbial Metabolite

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Serotonin (5-Hydroxytryptamine) hydrochloride is a monoamine neurotransmitter in the CNS and an endogenous 5-HT receptor agonist, with blood-brain barrier permeability. Serotonin hydrochloride is also a catechol O-methyltransferase (COMT) inhibitor with a Ki of 44 μM.

    IC50 & Target

    Human Endogenous Metabolite

     

    Microbial Metabolite

     

    serotonin

     

    In Vitro

    Serotonin hydrochloride is a monoamine neurotransmitter in the CNS and an endogenous 5-HT receptor agonist. Serotonin hydrochloride also inhibits catechol O-methyltransferase (COMT), an enzyme that contributes to modultion the perception of pain, via non-competitive binding to the site bound by catechol substrates with a binding affinity comparable to the binding affinity of catechol itself (Ki= 44 μM). Results show that addition of 100 μM of Serotonin hydrochloride decreases the reaction velocity of COMT[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Serotonin hydrochloride Related Antibodies
    In Vivo

    Serotonin hydrochloride produces robust hypersensitivity compare to saline-treated controls (p<0.001)[1].
    Due to the chemical structure of serotonin hydrochloride may prevent it from crossing the blood-brain barrier, but serotonin hydrochloride may utilize serotonin transport proteins to cross the endothelial cells of the blood-brain barrier[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    Molecular Weight

    212.68

    Formula

    C10H13ClN2O
    CAS No.
    Appearance

    Solid

    Color

    Off-white to light brown

    SMILES

    OC1=CC2=C(NC=C2CCN)C=C1.[H]Cl
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (470.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 100 mg/mL (470.19 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.7019 mL 23.5095 mL 47.0190 mL
    5 mM 0.9404 mL 4.7019 mL 9.4038 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    Volume (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (9.78 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (9.78 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.97%

    SDS (644 KB)

    COA (246 KB) HNMR (188 KB) LCMS (347 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [2]

    For macrophage culture, peritoneal cavity cells are collected from WT and IL-13-/- mice with or without dextran sodium sulfate (DSS) treatment and cultured. Cells are plated at a concentration of 3.0×106 cells per millilitre, and treated with either lipopolysaccharides (LPS); (100 ng/mL) or Serotonin hydrochloride (10-10 M) for 24 hours. The culture supernatant are collected and stored in -80°C until determination of cytokine levels using protein array system[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [2]

    Dextran sodium sulfate (DSS) colitis is induced by orally administering 5% DSS in drinking water for 5 days. In a separate experiment, IL-13-/- mice are injected subcutaneously with 100 mg/kg of Serotonin hydrochloride (5-HTP) twice daily for 8 days beginning 3 days prior to induction of DSS colitis; whereas, the control IL-13-/- mice receive saline as vehicle. Animals are anaesthetized prior to euthanization via cervical dislocation at the conclusion of each experiment or if they reach a predetermined end point (ie, loss of ≥20% body weight and/or significant deterioration of body condition)[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 4.7019 mL 23.5095 mL 47.0190 mL 117.5475 mL
    5 mM 0.9404 mL 4.7019 mL 9.4038 mL 23.5095 mL
    10 mM 0.4702 mL 2.3509 mL 4.7019 mL 11.7547 mL
    15 mM 0.3135 mL 1.5673 mL 3.1346 mL 7.8365 mL
    20 mM 0.2351 mL 1.1755 mL 2.3509 mL 5.8774 mL
    25 mM 0.1881 mL 0.9404 mL 1.8808 mL 4.7019 mL
    30 mM 0.1567 mL 0.7836 mL 1.5673 mL 3.9182 mL
    40 mM 0.1175 mL 0.5877 mL 1.1755 mL 2.9387 mL
    50 mM 0.0940 mL 0.4702 mL 0.9404 mL 2.3509 mL
    60 mM 0.0784 mL 0.3918 mL 0.7836 mL 1.9591 mL
    80 mM 0.0588 mL 0.2939 mL 0.5877 mL 1.4693 mL
    100 mM 0.0470 mL 0.2351 mL 0.4702 mL 1.1755 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Serotonin hydrochloride Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Serotonin153-98-05-Hydroxytryptamine5-HT5-HT ReceptorCOMTEndogenous MetaboliteSerotonin Receptor5-hydroxytryptamine ReceptorCatechol-O-methyltransferaseInhibitorinhibitorinhibit

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