1. GPCR/G Protein
    Neuronal Signaling
    Metabolic Enzyme/Protease
  2. 5-HT Receptor
    COMT

Serotonin hydrochloride (Synonyms: 5-Hydroxytryptamine hydrochloride; 5-HT hydrochloride)

Cat. No.: HY-B1473 Purity: 99.97%
Handling Instructions

Serotonin hydrochloride is a monoamine neurotransmitter in the CNS and an endogenous 5-HT receptor agonist. Serotonin hydrochloride is also a catechol O-methyltransferase (COMT) inhibitor with a Ki of 44 μM.

For research use only. We do not sell to patients.

Serotonin hydrochloride Chemical Structure

Serotonin hydrochloride Chemical Structure

CAS No. : 153-98-0

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
50 mg USD 50 In-stock
Estimated Time of Arrival: December 31
100 mg USD 65 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Serotonin hydrochloride is a monoamine neurotransmitter in the CNS and an endogenous 5-HT receptor agonist. Serotonin hydrochloride is also a catechol O-methyltransferase (COMT) inhibitor with a Ki of 44 μM.

IC50 & Target

5-HT receptor[1]
Ki: 44 μM (COMT)[1]

In Vitro

Serotonin hydrochloride is a monoamine neurotransmitter in the CNS and an endogenous 5-HT receptor agonist. Serotonin hydrochloride also inhibits catechol O-methyltransferase (COMT), an enzyme that contributes to modultion the perception of pain, via non-competitive binding to the site bound by catechol substrates with a binding affinity comparable to the binding affinity of catechol itself (Ki= 44 μM). Results show that addition of 100 μM of Serotonin hydrochloride decreases the reaction velocity of COMT[1].

In Vivo

Serotonin hydrochloride produces robust hypersensitivity compare to saline-treated controls (p<0.001)[1]. A significant increase in colonic 5-HT content is observed in IL-13-/- mice receiving Serotonin hydrochloride compare to IL-13-/- receiving vehicle following induction of DSS colitis[2].

Clinical Trial
Solvent & Solubility
In Vitro: 

DMSO : 150 mg/mL (705.28 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.7019 mL 23.5095 mL 47.0190 mL
5 mM 0.9404 mL 4.7019 mL 9.4038 mL
10 mM 0.4702 mL 2.3510 mL 4.7019 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.58 mg/mL (12.13 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: 2.58 mg/mL (12.13 mM); Clear solution; Need warming

  • 3.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.58 mg/mL (12.13 mM); Clear solution

References
Cell Assay
[2]

For macrophage culture, peritoneal cavity cells are collected from WT and IL-13-/- mice with or without dextran sodium sulfate (DSS) treatment and cultured. Cells are plated at a concentration of 3.0×106 cells per millilitre, and treated with either lipopolysaccharides (LPS); (100 ng/mL) or Serotonin hydrochloride (10-10 M) for 24 hours. The culture supernatant are collected and stored in -80°C until determination of cytokine levels using protein array system[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[2]

Dextran sodium sulfate (DSS) colitis is induced by orally administering 5% DSS in drinking water for 5 days. In a separate experiment, IL-13-/- mice are injected subcutaneously with 100 mg/kg of Serotonin hydrochloride (5-HTP) twice daily for 8 days beginning 3 days prior to induction of DSS colitis; whereas, the control IL-13-/- mice receive saline as vehicle. Animals are anaesthetized prior to euthanization via cervical dislocation at the conclusion of each experiment or if they reach a predetermined end point (ie, loss of ≥20% body weight and/or significant deterioration of body condition)[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

212.68

Formula

C₁₀H₁₃ClN₂O

CAS No.

153-98-0

SMILES

OC1=CC2=C(NC=C2CCN)C=C1.[H]Cl

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Purity: 99.97%

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Product Name:
Serotonin hydrochloride
Cat. No.:
HY-B1473
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Serotonin hydrochloride

Cat. No.: HY-B1473