Acetylcholine chloride
Based on 33 publication(s) in Google Scholar
Acetylcholine chloride (ACh chloride), a neurotransmitter, is a potent and BBB-permeable cholinergic agonist. Acetylcholine chloride is a modulator of the activity of dopaminergic (DAergic) neurons through the stimulation of nicotinic acetylcholine receptors (nAChRs). Acetylcholine chloride inhibits p53 mutant peptide aggregation in vitro.
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- Pureté : 99.91%
- CAS No.: 60-31-1
- Formule: C7H16ClNO2
- Masse moléculaire:181.66
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Stockage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
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Phorbol 12-myristate 13-acetatePKC Activator -
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Acetylcholine chloride (Standard)Cholinergic Agonist -
HY-P0201
Substance PNeurotransmitter -
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Isoprenaline hydrochlorideβ-adrenoceptor Agonist
Publications Citing Use of MedChemExpress (MCE) Acetylcholine chloride
More- Cancer Cell. 2025 Nov 10;43(11):2089-2105.e10. [Abstract]
- Cell. 2026 Mar 5;189(5):1323-1340.e25. [Abstract]
- Cell Res. 2026 Apr;36(4):286-299. [Abstract]
- Cell Stem Cell. 2026 Aug 6;33(8):1363-1378.e9.
- Trends Biotechnol. 2025 Apr;43(4):918-945. [Abstract]
- Redox Biol. 2024 Feb:69:103004. [Abstract]
- J Nanobiotechnology. 2026 Jun 30. [Abstract]
- MedComm (2020). 2026 Mar 28;7(4):e70706. [Abstract]
- Phytomedicine. 2026 Jan 17:153:157846. [Abstract]
- J Hazard Mater. 2024 Nov 5:479:135690. [Abstract]
- J Hazard Mater. 2024 Mar 5:465:133248. [Abstract]
- Acta Pharmacol Sin. 2025 Mar 26. [Abstract]
- Proc Natl Acad Sci U S A. 2024 Jul 2;121(27):e2402143121. [Abstract]
- Oncogene. 2026 Jul;45(26):2611-2625.
- Curr Biol. 2026 May 4;36(9):2367-2381.e9. [Abstract]
- J Ethnopharmacol. 2025 Jan 30;337(Pt 1):118707. [Abstract]
- Mol Metab. 2026 May 26:102384. [Abstract]
- Mol Metab. 2023 Dec:78:101811. [Abstract]
- Cells. 2024 Oct 14;13(20):1697. [Abstract]
- Int J Mol Sci. 2026 Apr 9;27(8):3381. [Abstract]
- Biochim Biophys Acta Mol Basis Dis. 2024 Dec;1870(8):167488. [Abstract]
- ACS Pharmacol Transl Sci. 2025 Jun 6;8(7):2048-2060. [Abstract]
- Mol Cell Endocrinol. 2025 Apr 1:599:112464. [Abstract]
- BMC Pulm Med. 2021 Jun 5;21(1):189. [Abstract]
- Front Cardiovasc Med. 2021 Jun 16:8:679240. [Abstract]
- PLoS One. 2026 Jul 2;21(7):e0351145.
- Clin Exp Pharmacol Physiol. 2025 Nov;52(11):e70081. [Abstract]
- Turk J Med Sci. 2025 Feb 19;55(2):509-517. [Abstract]
- Uppsala University. 2026.
- UCSF. 2025.
- Res Sq. 2025 Nov 12.
- Biomed Pharmacother. 2024 Aug:177:117105. [Abstract]
- bioRxiv. 2023 Oct 13.
Customer Validation & Images
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In Vivo Efficacy Study
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IF
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Flow Cytometry
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In Vivo Efficacy Study
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Cell Proliferation/Viability Assay
Voir tous les produits spécifiques à Isoform Calcium Channel
MoreVoir tous les produits spécifiques à Isoform Endogenous Metabolite
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Activité biologique
Description
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Human Endogenous Metabolite |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| HEK293 | EC50 |
0.22 μM
Compound: ACh
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Activation of M3-ACh receptor-FLASH/CFP expressed in HEK293 cells assessed as FRET signal
Activation of M3-ACh receptor-FLASH/CFP expressed in HEK293 cells assessed as FRET signal
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[PMID: 20716489] |
| HEK293 | EC50 |
0.77 μM
Compound: ACh
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Activation of M1-ACh receptor-FLASH/CFP expressed in HEK293 cells assessed as FRET signal
Activation of M1-ACh receptor-FLASH/CFP expressed in HEK293 cells assessed as FRET signal
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[PMID: 20716489] |
| HEK293 | EC50 |
0.79 μM
Compound: ACh
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Activation of M5-ACh receptor-FLASH/CFP expressed in HEK293 cells assessed as FRET signal
Activation of M5-ACh receptor-FLASH/CFP expressed in HEK293 cells assessed as FRET signal
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[PMID: 20716489] |
In Vitro
Acetylcholine chloride (ACh chloride; 10 μM) opens the calcium channel and the fluorescence value and intracellular free calcium will increase significantly when the medium is with high calcium, while these will decrease when the medium is without calcium in sweat gland epithelial cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male and female out-bred albino mice weighing 18-22 g (sepsis)[4]
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Dosage:20 mg/kg
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Administration:SC; single dose
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Result:Significantly reduces mortality of mice from sepsis induced by intraperitoneal injection of 2×109 E. coli bacterial bodies and the blood levels of proinflammatory cytokines TNF-α, IL-1β, and IL-6.
Chemical Information
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CAS No. 60-31-1
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Appearance Solid
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Masse moléculaire 181.66
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Formule C7H16ClNO2
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Color White to off-white
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SMILES
O=C(OCC[N+](C)(C)C)C.[Cl-]
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Synonyms
ACh chloride
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (33)
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Journal Impact Factor
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Most Recent
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Cancer Cell
2025 Nov 10;43(11):2089-2105.e10. PMID: 40829591 -
Cell
Sympathetic-epithelial crosstalk governs tissue-resident memory T cell immunosurveillance in the skin. [Abstract]2026 Mar 5;189(5):1323-1340.e25. PMID: 41616781 -
Cell Res
Light sensing enhances thermotolerance and competitive fitness via serotonergic signaling in an eyeless organism. [Abstract]2026 Apr;36(4):286-299. PMID: 41764323 -
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Trends Biotechnol
Programmable embedded bioprinting for one-step manufacturing of arterial models with customized contractile and metabolic functions. [Abstract]2025 Apr;43(4):918-945. PMID: 39779422 -
Redox Biol
Myeloid ACE2 protects against septic hypotension and vascular dysfunction through Ang-(1-7)-Mas-mediated macrophage polarization. [Abstract]2024 Feb:69:103004. PMID: 38141575 -
J Nanobiotechnology
Neutrophil membrane-mediated biomimetic nanoparticle for co-delivery of icariin and siORMDL3 attenuates asthma exacerbated by respiratory syncytial virus. [Abstract]2026 Jun 30. PMID: 42381004 -
MedComm (2020)
2026 Mar 28;7(4):e70706. PMID: 41930327 -
Phytomedicine
Andrographolide ameliorates vascular injury in hypertension by suppressing EndMT via Sp1-Notch1 signaling. [Abstract]2026 Jan 17:153:157846. PMID: 41653618 -
J Hazard Mater
β-ionone inhibits the grazing of Daphnia sinensis by reducing the activity of acetylcholinesterase. [Abstract]2024 Nov 5:479:135690. PMID: 39255669 -
J Hazard Mater
β-cyclocitral, a novel AChE inhibitor, contributes to the defense of Microcystis aeruginosa against Daphnia grazing. [Abstract]2024 Mar 5:465:133248. PMID: 38147752 -
Acta Pharmacol Sin
Huperzine A attenuates epileptic seizures via enhancing dCA1-projecting septal cholinergic transmission. [Abstract]2025 Mar 26. PMID: 40140526
Acetylcholine chloride purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2025 Mar 26. [Abstract]
Acetylcholine (ACh, 20 μM; 250 nL, locally injected into the dorsal CA1 (dCA1)). AAV-EF1α-FLEX-taCasp3 virus was injected in the medial septum (MS) and the cannula was implanted in the dCA1 for Hup A (0.1 -1 mg/kg; i.p.) intervention. Effects of Hup A on the development of seizure stage (Left) and ADDs (Right) during kindling acquisition, in the condition of AAV-taCasp3-mediated apoptosis of the ChAT+ neurons in MS, n=9.
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Proc Natl Acad Sci U S A
2024 Jul 2;121(27):e2402143121. PMID: 38923993
Acetylcholine chloride purchased from MedChemExpress. Usage Cited in: Proc Natl Acad Sci U S A. 2024 Jul 2;121(27):e2402143121. [Abstract]
The impact of ACh (Acetylcholine, 1 μM; 24 h) on the uptake of latex beads-rabbit IgG-FITC complex (green) in murine BMDMs (red; Upper) or human MDMs (red; Lower) was assessed. The data are presented as the percentage of latex beads-rabbit IgG-FITC-positive cells relative to the total cell count. (Scale bar, 100 μm.)
Acetylcholine chloride purchased from MedChemExpress. Usage Cited in: Proc Natl Acad Sci U S A. 2024 Jul 2;121(27):e2402143121. [Abstract]
BMDMs from ChAT-GFP mice were treated with Pam3, or in combination with ACh (Acetylcholine, 10 μM), for 24 h and subsequently evaluated for GFP expression.
Acetylcholine chloride purchased from MedChemExpress. Usage Cited in: Proc Natl Acad Sci U S A. 2024 Jul 2;121(27):e2402143121. [Abstract]
ACh (Acetylcholine, 5 mg/kg; i.p., once daily for 3 days). The number of apoptotic neutrophils was assessed by flow cytometry at day 3 post-Pam3 stimulation in Chatfl/fl and Chatfl/flLyz2cre mice.
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Curr Biol
2026 May 4;36(9):2367-2381.e9. PMID: 42025171 -
J Ethnopharmacol
Mailuoning oral liquid ameliorates vasculitis in thromboangiitis obliterans rats via inactivating cGAS-STING-IRF3 and TLR4-MAPKs/NF-κB signaling pathways. [Abstract]2025 Jan 30;337(Pt 1):118707. PMID: 39181282 -
Mol Metab
Vagal control of the brain-esophagus axis ameliorates stress-induced esophageal motility dysfunction in male mice. [Abstract]2026 May 26:102384. PMID: 42203107 -
Mol Metab
Deletion of Ascl1 in pancreatic β-cells improves insulin secretion, promotes parasympathetic innervation, and attenuates dedifferentiation during metabolic stress. [Abstract]2023 Dec:78:101811. PMID: 37769990 -
Cells
Single-Cell Hypertrophy Promotes Contractile Function of Cultured Human Airway Smooth Muscle Cells via Piezo1 and YAP Auto-Regulation. [Abstract]2024 Oct 14;13(20):1697. PMID: 39451215 -
Int J Mol Sci
A Clozapine-Responsive GPCR-Based Gene Switch for Pharmacological Control of Gene Expression in Mammalian Cells and In Vivo. [Abstract]2026 Apr 9;27(8):3381. PMID: 42074026 -
Biochim Biophys Acta Mol Basis Dis
Dietary apigenin ameliorates obesity-related hypertension through TRPV4-dependent vasorelaxation and TRPV4-independent adiponectin secretion. [Abstract]2024 Dec;1870(8):167488. PMID: 39218272 -
ACS Pharmacol Transl Sci
Critical Residues in the Gβ Subunit Mediate Selective Recruitment of the Gαq Heterotrimer to Activated G Protein-Coupled Receptors. [Abstract]2025 Jun 6;8(7):2048-2060. PMID: 40672669 -
Mol Cell Endocrinol
Vagotomy suppresses food intake by increasing GLP-1 secretion via the M3 AChR-AMPKα pathway in mice. [Abstract]2025 Apr 1:599:112464. PMID: 39848433 -
BMC Pulm Med
Opposing responses of the rat pulmonary artery and vein to phenylephrine and other agents in vitro. [Abstract]2021 Jun 5;21(1):189. PMID: 34090386 -
Front Cardiovasc Med
2021 Jun 16:8:679240. PMID: 34222374 -
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Clin Exp Pharmacol Physiol
Indole-3-Propionic Acid Improves Vascular Function in High-Fat Diet-Induced Obese Mice via eNOS. [Abstract]2025 Nov;52(11):e70081. PMID: 41057979 -
Turk J Med Sci
2025 Feb 19;55(2):509-517. PMID: 40342326 -
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Biomed Pharmacother
Spinosad blocks CHRNA5 mediated EGFR signaling pathway activation to inhibit lung adenocarcinoma proliferation. [Abstract]2024 Aug:177:117105. PMID: 39002438
Acetylcholine chloride purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Aug:177:117105. [Abstract]
The addition of exogenous Acetylcholine (1-10 μM) reversed the decline in H1975 cell viability induced by Spinosad (10 μM).
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Solvant et solubilité
In Vitro:
H2O : 100 mg/mL (550.48 mM; Need ultrasonic)
DMSO : 62.5 mg/mL (344.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (11.45 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (11.45 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 23.33 mg/mL (128.43 mM); Clear solution; Need ultrasonic
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Pureté et documentation
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Fiche technique (274 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Instruction de manipulation (2659 KB)
Références
[2]. A Young, et al. Diarrhoea of famine and malnutrition--investigations using a rat model. 2--Ileal hypersecretion induced by starvation. Gut. 1990 Feb;31(2):162-9. [Content Brief]
[3]. Xia Lei, et al. Effects of acetylcholine chloride on intracellular calcium concentration of cultured sweat gland epithelial cells. Arch Dermatol Res. 2008 Aug;300(7):335-41. [Content Brief]
[4]. P F Zabrodskii, et al. Effect of acetylcholine on mortality of mice from sepsis and proinflammatory cytokine production. Bull Exp Biol Med. 2011 Jan;150(3):340-2. [Content Brief]
[5]. Zhaolin Chen, et al. Inhibition of p53 Mutant Peptide Aggregation In Vitro by Cationic Osmolyte Acetylcholine Chloride. Protein Pept Lett. 2017;24(4):353-357. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 5.5048 mL | 27.5239 mL | 55.0479 mL | 137.6197 mL |
| 5 mM | 1.1010 mL | 5.5048 mL | 11.0096 mL | 27.5239 mL | |
| 10 mM | 0.5505 mL | 2.7524 mL | 5.5048 mL | 13.7620 mL | |
| 15 mM | 0.3670 mL | 1.8349 mL | 3.6699 mL | 9.1746 mL | |
| 20 mM | 0.2752 mL | 1.3762 mL | 2.7524 mL | 6.8810 mL | |
| 25 mM | 0.2202 mL | 1.1010 mL | 2.2019 mL | 5.5048 mL | |
| 30 mM | 0.1835 mL | 0.9175 mL | 1.8349 mL | 4.5873 mL | |
| 40 mM | 0.1376 mL | 0.6881 mL | 1.3762 mL | 3.4405 mL | |
| 50 mM | 0.1101 mL | 0.5505 mL | 1.1010 mL | 2.7524 mL | |
| 60 mM | 0.0917 mL | 0.4587 mL | 0.9175 mL | 2.2937 mL | |
| 80 mM | 0.0688 mL | 0.3440 mL | 0.6881 mL | 1.7202 mL | |
| 100 mM | 0.0550 mL | 0.2752 mL | 0.5505 mL | 1.3762 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Acetylcholine chloride Related Classifications
HY-B0282 Related Classifications
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