2. Membrane Transporter/Ion Channel Neuronal Signaling Metabolic Enzyme/Protease
  3. nAChR Calcium Channel Endogenous Metabolite
  4. Acetylcholine chloride

Acetylcholine chloride  (Synonyms: ACh chloride)

Cat. No.: HY-B0282 Purity: 99.91%
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Acetylcholine chloride (ACh chloride), a neurotransmitter, is a potent and BBB-permeable cholinergic agonist. Acetylcholine chloride is a modulator of the activity of dopaminergic (DAergic) neurons through the stimulation of nicotinic acetylcholine receptors (nAChRs). Acetylcholine chloride inhibits p53 mutant peptide aggregation in vitro.

For research use only. We do not sell to patients.

CAS No. : 60-31-1

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
500 mg In-stock
1 g In-stock
5 g In-stock
10 g In-stock
50 g   Get quote  

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Customer Review

Based on 24 publication(s) in Google Scholar

Other Forms of Acetylcholine chloride:

Acetylcholine chloride Related Antibodies

Top Publications Citing Use of Products

    Acetylcholine chloride purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2025 Mar 26.  [Abstract]

    Acetylcholine (ACh, 20 μM; 250 nL, locally injected into the dorsal CA1 (dCA1)). AAV-EF1α-FLEX-taCasp3 virus was injected in the medial septum (MS) and the cannula was implanted in the dCA1 for Hup A (0.1 -1 mg/kg; i.p.) intervention. Effects of Hup A on the development of seizure stage (Left) and ADDs (Right) during kindling acquisition, in the condition of AAV-taCasp3-mediated apoptosis of the ChAT+ neurons in MS, n=9.

    Acetylcholine chloride purchased from MedChemExpress. Usage Cited in: Proc Natl Acad Sci U S A. 2024 Jul 2;121(27):e2402143121.

    The impact of ACh (Acetylcholine, 1 μM; 24 h) on the uptake of latex beads-rabbit IgG-FITC complex (green) in murine BMDMs (red; Upper) or human MDMs (red; Lower) was assessed. The data are presented as the percentage of latex beads-rabbit IgG-FITC-positive cells relative to the total cell count. (Scale bar, 100 μm.)

    Acetylcholine chloride purchased from MedChemExpress. Usage Cited in: Proc Natl Acad Sci U S A. 2024 Jul 2;121(27):e2402143121.

    BMDMs from ChAT-GFP mice were treated with Pam3, or in combination with ACh (Acetylcholine, 10 μM), for 24 h and subsequently evaluated for GFP expression.

    Acetylcholine chloride purchased from MedChemExpress. Usage Cited in: Proc Natl Acad Sci U S A. 2024 Jul 2;121(27):e2402143121.

    ACh (Acetylcholine, 5 mg/kg; i.p., once daily for 3 days). The number of apoptotic neutrophils was assessed by flow cytometry at day 3 post-Pam3 stimulation in Chatfl/fl and Chatfl/flLyz2cre mice.

    Acetylcholine chloride purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Jul 12:177:117105.  [Abstract]

    The addition of exogenous Acetylcholine (1-10 μM) reversed the decline in H1975 cell viability induced by Spinosad (10 μM).

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    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Acetylcholine chloride (ACh chloride), a neurotransmitter, is a potent and BBB-permeable cholinergic agonist. Acetylcholine chloride is a modulator of the activity of dopaminergic (DAergic) neurons through the stimulation of nicotinic acetylcholine receptors (nAChRs)[1][2]. Acetylcholine chloride inhibits p53 mutant peptide aggregation in vitro[5].

    IC50 & Target[1][5]

    Human Endogenous Metabolite

     

    Cellular Effect
    Cell Line Type Value Description References
    HEK293 EC50
    0.22 μM
    Compound: ACh
    Activation of M3-ACh receptor-FLASH/CFP expressed in HEK293 cells assessed as FRET signal
    Activation of M3-ACh receptor-FLASH/CFP expressed in HEK293 cells assessed as FRET signal
    		[PMID: 20716489]
    HEK293 EC50
    0.77 μM
    Compound: ACh
    Activation of M1-ACh receptor-FLASH/CFP expressed in HEK293 cells assessed as FRET signal
    Activation of M1-ACh receptor-FLASH/CFP expressed in HEK293 cells assessed as FRET signal
    		[PMID: 20716489]
    HEK293 EC50
    0.79 μM
    Compound: ACh
    Activation of M5-ACh receptor-FLASH/CFP expressed in HEK293 cells assessed as FRET signal
    Activation of M5-ACh receptor-FLASH/CFP expressed in HEK293 cells assessed as FRET signal
    		[PMID: 20716489]
    In Vitro

    Acetylcholine chloride (ACh chloride; 10 μM) opens the calcium channel and the fluorescence value and intracellular free calcium will increase significantly when the medium is with high calcium, while these will decrease when the medium is without calcium in sweat gland epithelial cells[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Acetylcholine chloride Related Antibodies
    In Vivo

    Acetylcholine chloride (ACh chloride; SC; 20 mg/kg; single dose) induces pronounced cholinergic stimulation and increase of mouse survival in experimental infection[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male and female out-bred albino mice weighing 18-22 g (sepsis)[4]
    Dosage: 20 mg/kg
    Administration: SC; single dose
    Result: Significantly reduces mortality of mice from sepsis induced by intraperitoneal injection of 2×109 E. coli bacterial bodies and the blood levels of proinflammatory cytokines TNF-α, IL-1β, and IL-6.
    Molecular Weight

    181.66

    Formula

    C7H16ClNO2
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(OCC[N+](C)(C)C)C.[Cl-]
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    H2O : 100 mg/mL (550.48 mM; Need ultrasonic)

    DMSO : 62.5 mg/mL (344.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 5.5048 mL 27.5239 mL 55.0479 mL
    5 mM 1.1010 mL 5.5048 mL 11.0096 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (11.45 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (11.45 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 23.33 mg/mL (128.43 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.91%

    SDS (396 KB)

    COA (245 KB) HNMR (238 KB) RP-HPLC (216 KB) MS (69 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 5.5048 mL 27.5239 mL 55.0479 mL 137.6197 mL
    5 mM 1.1010 mL 5.5048 mL 11.0096 mL 27.5239 mL
    10 mM 0.5505 mL 2.7524 mL 5.5048 mL 13.7620 mL
    15 mM 0.3670 mL 1.8349 mL 3.6699 mL 9.1746 mL
    20 mM 0.2752 mL 1.3762 mL 2.7524 mL 6.8810 mL
    25 mM 0.2202 mL 1.1010 mL 2.2019 mL 5.5048 mL
    30 mM 0.1835 mL 0.9175 mL 1.8349 mL 4.5873 mL
    40 mM 0.1376 mL 0.6881 mL 1.3762 mL 3.4405 mL
    50 mM 0.1101 mL 0.5505 mL 1.1010 mL 2.7524 mL
    60 mM 0.0917 mL 0.4587 mL 0.9175 mL 2.2937 mL
    80 mM 0.0688 mL 0.3440 mL 0.6881 mL 1.7202 mL
    100 mM 0.0550 mL 0.2752 mL 0.5505 mL 1.3762 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Acetylcholine chloride Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Acetylcholine60-31-1AChnAChRCalcium ChannelEndogenous MetaboliteNicotinic acetylcholine receptorsCa2+ channelsCa channelsfluorescenceglandepithelialsepsisInhibitorinhibitorinhibit

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