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Acetylcholine bromide  (Synonyms: ACh bromide)

Cat. No.: HY-B0282A Purity: 99.02%
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Acetylcholine bromide is an organic choline salt compound commonly used in neurophysiology and pharmacology experiments. It plays an important role in the nervous system, acting as a neurotransmitter that transmits signals and regulates many important physiological functions. Due to its special biological activity, Acetylcholine bromide is widely used in the study of neurotransmitters, nerve damage and muscle movement.

For research use only. We do not sell to patients.

CAS No. : 66-23-9

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Based on 24 publication(s) in Google Scholar

Other Forms of Acetylcholine bromide:

Acetylcholine bromide Related Antibodies

Top Publications Citing Use of Products

    Acetylcholine bromide purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2025 Mar 26.  [Abstract]

    Acetylcholine (ACh, 20 μM; 250 nL, locally injected into the dorsal CA1 (dCA1)). AAV-EF1α-FLEX-taCasp3 virus was injected in the medial septum (MS) and the cannula was implanted in the dCA1 for Hup A (0.1 -1 mg/kg; i.p.) intervention. Effects of Hup A on the development of seizure stage (Left) and ADDs (Right) during kindling acquisition, in the condition of AAV-taCasp3-mediated apoptosis of the ChAT+ neurons in MS, n=9.

    Acetylcholine bromide purchased from MedChemExpress. Usage Cited in: Proc Natl Acad Sci U S A. 2024 Jul 2;121(27):e2402143121.

    The impact of ACh (Acetylcholine, 1 μM; 24 h) on the uptake of latex beads-rabbit IgG-FITC complex (green) in murine BMDMs (red; Upper) or human MDMs (red; Lower) was assessed. The data are presented as the percentage of latex beads-rabbit IgG-FITC-positive cells relative to the total cell count. (Scale bar, 100 μm.)

    Acetylcholine bromide purchased from MedChemExpress. Usage Cited in: Proc Natl Acad Sci U S A. 2024 Jul 2;121(27):e2402143121.

    BMDMs from ChAT-GFP mice were treated with Pam3, or in combination with ACh (Acetylcholine, 10 μM), for 24 h and subsequently evaluated for GFP expression.

    Acetylcholine bromide purchased from MedChemExpress. Usage Cited in: Proc Natl Acad Sci U S A. 2024 Jul 2;121(27):e2402143121.

    ACh (Acetylcholine, 5 mg/kg; i.p., once daily for 3 days). The number of apoptotic neutrophils was assessed by flow cytometry at day 3 post-Pam3 stimulation in Chatfl/fl and Chatfl/flLyz2cre mice.

    Acetylcholine bromide purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Jul 12:177:117105.  [Abstract]

    The addition of exogenous Acetylcholine (1-10 μM) reversed the decline in H1975 cell viability induced by Spinosad (10 μM).

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    Description

    Acetylcholine bromide is an organic choline salt compound commonly used in neurophysiology and pharmacology experiments. It plays an important role in the nervous system, acting as a neurotransmitter that transmits signals and regulates many important physiological functions. Due to its special biological activity, Acetylcholine bromide is widely used in the study of neurotransmitters, nerve damage and muscle movement.

    In Vitro

    Acetylcholine (bromide) is a biochemical reagent that can be used as a biological material or organic compound for life science related research.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Acetylcholine bromide Related Antibodies
    Clinical Trial
    Molecular Weight

    226.11

    Formula

    C7H16BrNO2
    CAS No.
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    O=C(OCC[N+](C)(C)C)C.[Br-]
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    H2O : 250 mg/mL (1105.66 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.4226 mL 22.1131 mL 44.2263 mL
    5 mM 0.8845 mL 4.4226 mL 8.8453 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Dosing volume
    (per animal)

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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.02%

    SDS (393 KB)

    COA (245 KB) HNMR (230 KB) RP-HPLC (206 KB) MS (69 KB)

    Handling Instructions (2659 KB)

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 4.4226 mL 22.1131 mL 44.2263 mL 110.5657 mL
    5 mM 0.8845 mL 4.4226 mL 8.8453 mL 22.1131 mL
    10 mM 0.4423 mL 2.2113 mL 4.4226 mL 11.0566 mL
    15 mM 0.2948 mL 1.4742 mL 2.9484 mL 7.3710 mL
    20 mM 0.2211 mL 1.1057 mL 2.2113 mL 5.5283 mL
    25 mM 0.1769 mL 0.8845 mL 1.7691 mL 4.4226 mL
    30 mM 0.1474 mL 0.7371 mL 1.4742 mL 3.6855 mL
    40 mM 0.1106 mL 0.5528 mL 1.1057 mL 2.7641 mL
    50 mM 0.0885 mL 0.4423 mL 0.8845 mL 2.2113 mL
    60 mM 0.0737 mL 0.3686 mL 0.7371 mL 1.8428 mL
    80 mM 0.0553 mL 0.2764 mL 0.5528 mL 1.3821 mL
    100 mM 0.0442 mL 0.2211 mL 0.4423 mL 1.1057 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Acetylcholine bromide Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Acetylcholine66-23-9AChBiochemical Assay Reagentsbiochemical assayreagentInhibitorinhibitorinhibit

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