2. Metabolic Enzyme/Protease Neuronal Signaling GPCR/G Protein
  3. Endogenous Metabolite mAChR
  4. Acetylcholine iodide

Acetylcholine iodide  (Synonyms: ACh iodide)

Cat. No.: HY-101086 Purity: 98.59%
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Acetylcholine iodide is a muscarinic receptor modulator. Acetylcholine iodide specifically binds to muscarinic receptors, inhibits sodium absorption, and induces chloride secretion. Acetylcholine iodide changes intestinal ion transport, enhances intestinal secretory function, induces or maintains mammary gland development and lactation. Acetylcholine iodide can be used for intestinal ion transport regulation and mammary gland physiological function research.

For research use only. We do not sell to patients.

CAS No. : 2260-50-6

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
100 mg In-stock
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Based on 24 publication(s) in Google Scholar

Other Forms of Acetylcholine iodide:

Acetylcholine iodide Related Antibodies

Top Publications Citing Use of Products

    Acetylcholine iodide purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2025 Mar 26.  [Abstract]

    Acetylcholine (ACh, 20 μM; 250 nL, locally injected into the dorsal CA1 (dCA1)). AAV-EF1α-FLEX-taCasp3 virus was injected in the medial septum (MS) and the cannula was implanted in the dCA1 for Hup A (0.1 -1 mg/kg; i.p.) intervention. Effects of Hup A on the development of seizure stage (Left) and ADDs (Right) during kindling acquisition, in the condition of AAV-taCasp3-mediated apoptosis of the ChAT+ neurons in MS, n=9.

    Acetylcholine iodide purchased from MedChemExpress. Usage Cited in: Proc Natl Acad Sci U S A. 2024 Jul 2;121(27):e2402143121.

    The impact of ACh (Acetylcholine, 1 μM; 24 h) on the uptake of latex beads-rabbit IgG-FITC complex (green) in murine BMDMs (red; Upper) or human MDMs (red; Lower) was assessed. The data are presented as the percentage of latex beads-rabbit IgG-FITC-positive cells relative to the total cell count. (Scale bar, 100 μm.)

    Acetylcholine iodide purchased from MedChemExpress. Usage Cited in: Proc Natl Acad Sci U S A. 2024 Jul 2;121(27):e2402143121.

    BMDMs from ChAT-GFP mice were treated with Pam3, or in combination with ACh (Acetylcholine, 10 μM), for 24 h and subsequently evaluated for GFP expression.

    Acetylcholine iodide purchased from MedChemExpress. Usage Cited in: Proc Natl Acad Sci U S A. 2024 Jul 2;121(27):e2402143121.

    ACh (Acetylcholine, 5 mg/kg; i.p., once daily for 3 days). The number of apoptotic neutrophils was assessed by flow cytometry at day 3 post-Pam3 stimulation in Chatfl/fl and Chatfl/flLyz2cre mice.

    Acetylcholine iodide purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Jul 12:177:117105.  [Abstract]

    The addition of exogenous Acetylcholine (1-10 μM) reversed the decline in H1975 cell viability induced by Spinosad (10 μM).

    View All Endogenous Metabolite Isoform Specific Products:

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    Human Endogenous Metabolite Microbial Metabolite

    View All mAChR Isoform Specific Products:

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    mAChR1 mAChR2 mAChR3 mAChR4 mAChR5

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Acetylcholine iodide is a muscarinic receptor modulator. Acetylcholine iodide specifically binds to muscarinic receptors, inhibits sodium absorption, and induces chloride secretion. Acetylcholine iodide changes intestinal ion transport, enhances intestinal secretory function, induces or maintains mammary gland development and lactation. Acetylcholine iodide can be used for intestinal ion transport regulation and mammary gland physiological function research[1][2][3].

    IC50 & Target

    Human Endogenous Metabolite

     

    In Vitro

    Acetylcholine iodide (25 mM; added to the serosal fluid of the rat proximal colonic eversion sac) increases the potential difference in the rat proximal colon[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Acetylcholine iodide Related Antibodies
    In Vivo

    Acetylcholine iodide (3 μg; i.v.) increases the electrical potential difference in the proximal colon of rats[2].
    Acetylcholine iodide (25 mg/kg; subcutaneous injection; twice a day; 15 days) induces mammary ductal growth in some virgin rats[3].
    Acetylcholine iodide (25 mg/kg; subcutaneous injection; twice a day; 5 days) induces lobular-acinar growth and secretion in the mammary glands of virgin rats pretreated with estrogen[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Female albino rats (Carworth, weighing 175 - 200 g, age unspecified) for studying mammary growth and lactation induction; Female albino rats (Carworth, postparturient, litter removed on 4th day after parturition) for studying maintenance of mammary structure and secretion[3].
    Dosage: 25 mg/kg, 50 mg/kg (dissolved in an unspecified solvent for subcutaneous injection)
    Administration: Subcutaneous injection, twice daily for 15 days (for induction in virgin rats), twice daily for 5 days after 10 days of estradiol pretreatment (for induction in estrogen-primed virgin rats), twice daily for 10 days (for maintenance in lactating rats after litter removal)
    Result: Induced duct growth in some cases without estradiol priming, and lobule-alveolar growth and secretion with estradiol priming in virgin rats.
    Retarded mammary involution and maintained lactation in lactating rats after litter removal.
    Molecular Weight

    273.11

    Formula

    C7H16INO2
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(OCC[N+](C)(C)C)C.[I-]
    Structure Classification
    Initial Source

    A transmitter substance of nerve impulses within the central and peripheral nervous systems
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (366.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : ≥ 100 mg/mL (366.15 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.6615 mL 18.3076 mL 36.6153 mL
    5 mM 0.7323 mL 3.6615 mL 7.3231 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

    V1

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    Concentration (final)

    C2

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    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (9.15 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (9.15 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 3.6615 mL 18.3076 mL 36.6153 mL 91.5382 mL
    5 mM 0.7323 mL 3.6615 mL 7.3231 mL 18.3076 mL
    10 mM 0.3662 mL 1.8308 mL 3.6615 mL 9.1538 mL
    15 mM 0.2441 mL 1.2205 mL 2.4410 mL 6.1025 mL
    20 mM 0.1831 mL 0.9154 mL 1.8308 mL 4.5769 mL
    25 mM 0.1465 mL 0.7323 mL 1.4646 mL 3.6615 mL
    30 mM 0.1221 mL 0.6103 mL 1.2205 mL 3.0513 mL
    40 mM 0.0915 mL 0.4577 mL 0.9154 mL 2.2885 mL
    50 mM 0.0732 mL 0.3662 mL 0.7323 mL 1.8308 mL
    60 mM 0.0610 mL 0.3051 mL 0.6103 mL 1.5256 mL
    80 mM 0.0458 mL 0.2288 mL 0.4577 mL 1.1442 mL
    100 mM 0.0366 mL 0.1831 mL 0.3662 mL 0.9154 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Acetylcholine iodide Related Classifications

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Acetylcholine2260-50-6AChEndogenous MetabolitemAChRMuscarinic acetylcholine receptormammary gland serosal fluid.Inhibitorinhibitorinhibit

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