2. Acetylcholine iodide

Acetylcholine iodide  (Synonyms: ACh iodide)

Cat. No.: HY-101086 Purity: 99.75%
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Acetylcholine iodide (ACh iodide) is a common neurotransmitter found in the central and peripheral nerve system.

For research use only. We do not sell to patients.

Acetylcholine iodide Chemical Structure

Acetylcholine iodide Chemical Structure

CAS No. : 2260-50-6

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Solid + Solvent
10 mM * 1 mL in DMSO
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Based on 6 publication(s) in Google Scholar

Other Forms of Acetylcholine iodide:

Acetylcholine iodide Related Antibodies

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Description

Acetylcholine iodide (ACh iodide) is a common neurotransmitter found in the central and peripheral nerve system[1].

Clinical Trial
Molecular Weight

273.11

Formula

C7H16INO2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(OCC[N+](C)(C)C)C.[I-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (366.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : ≥ 100 mg/mL (366.15 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.6615 mL 18.3076 mL 36.6153 mL
5 mM 0.7323 mL 3.6615 mL 7.3231 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (9.15 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (9.15 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 3.6615 mL 18.3076 mL 36.6153 mL 91.5382 mL
5 mM 0.7323 mL 3.6615 mL 7.3231 mL 18.3076 mL
10 mM 0.3662 mL 1.8308 mL 3.6615 mL 9.1538 mL
15 mM 0.2441 mL 1.2205 mL 2.4410 mL 6.1025 mL
20 mM 0.1831 mL 0.9154 mL 1.8308 mL 4.5769 mL
25 mM 0.1465 mL 0.7323 mL 1.4646 mL 3.6615 mL
30 mM 0.1221 mL 0.6103 mL 1.2205 mL 3.0513 mL
40 mM 0.0915 mL 0.4577 mL 0.9154 mL 2.2885 mL
50 mM 0.0732 mL 0.3662 mL 0.7323 mL 1.8308 mL
60 mM 0.0610 mL 0.3051 mL 0.6103 mL 1.5256 mL
80 mM 0.0458 mL 0.2288 mL 0.4577 mL 1.1442 mL
100 mM 0.0366 mL 0.1831 mL 0.3662 mL 0.9154 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Acetylcholine2260-50-6AChInhibitorinhibitorinhibit

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