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Folic acid  (Synonyms: Vitamin B9; Vitamin M)

Cat. No.: HY-16637 Purity: 99.25%
COA Handling Instructions

Folic acid (Vitamin B9) is a orally active essential nutrient from the B complex group of vitamins. Folic acid shows antidepressant-like effect. Folic acid sodium reduces the risk of neonatal neural tube defects. Folic acid can be used to the research of megaloblastic and macrocytic anemias due to folic deficiency.

For research use only. We do not sell to patients.

Folic acid Chemical Structure

Folic acid Chemical Structure

CAS No. : 59-30-3

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
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Customer Review

Based on 8 publication(s) in Google Scholar

Other Forms of Folic acid:

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  • Biological Activity

  • Purity & Documentation

  • References

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Description

Folic acid (Vitamin B9) is a orally active essential nutrient from the B complex group of vitamins. Folic acid shows antidepressant-like effect. Folic acid sodium reduces the risk of neonatal neural tube defects. Folic acid can be used to the research of megaloblastic and macrocytic anemias due to folic deficiency[1][2][3][4].

IC50 & Target

Human Endogenous Metabolite

 

Microbial Metabolite

 

In Vitro

Folic acid plays a critical role in the prevention of chromosome breakage and hypomethylation of DNA[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Folic acid (10, 50, 100 mg/kg; p.o.) shows an antidepressant-like effect in this behavioral mouse model[2].
Folic acid (1, 10 nmol/site) shows no psychostimulant effect in mice habituated to the novel environment[2].
Folic acid (1, 5 mg/kg; p.o.) prevents epigenetic modification of hepatic gene expression in the offspring in rats[3].When folic acid was administrated orally as aqueous solution in rat, the AUC was 1.4 μg h/mL and oral bioavailability is 35%[5].

Induction of Acute Kidney Injury (AKI)[6]

Background
Folic acid metabolism requires higher levels of NADPH to reduce folate to THF decreasing the antioxidant defense. The redox imbalance generated by folic acid metabolism is one of the main mechanisms involved in renal damage.
Specific Mmodeling Methods
Rat: Wistar • male
Administration: 300mg/ml • i.p. • single dose
Note
(1) Intraperitoneal injection 300 mg/kg folic acid (dissolved in 300 mM NaHCO3) in male Wistar rats with an initial body weight between 230 to 250 g.
(2) Plasma was collected and analyzed at days 2, 4, 7, 14 and 28 after folic acid administration.
Modeling Record
Metabolic changes : Assessment of renal injury by blood urea nitrogen (BUN) and plasma creatinine.
Individual phenotypic change: The ratio of kidney weight to total rat weight was detected.
Correlated Product(s): Acetylcysteine (HY-B0215)

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 30-40 g swiss mice[2]
Dosage: 10, 50, 100 mg/kg
Administration: Oral administration
Result: Decreased the immobility time in the forced swimming test (FST) (F324=11.21) and produced a significant effect in the immobility time in the tail suspension test (TST) (F3,20=5.71).
Animal Model: 30-40 g swiss mice[2]
Dosage: 1-10 nmol/site
Administration: Intracerebroventrical injection
Result: Decreased the immobility time of mice in the FST (F3,22=12.31) and TST (F3,22=5.50).
Animal Model: Virgin female Wistar rats[3]
Dosage: 1, 5 mg/kg (180 g/kg protein with 1 mg/kg folic acid or 90 g/kg casein with 1, 5 mg/kg folic acid)
Administration: Oral administration
Result: Prevented epigenetic modification of hepatic gene expression in the offspring.
Clinical Trial
Molecular Weight

441.40

Formula

C19H19N7O6

CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(O)CC[C@@H](C(O)=O)NC(C1=CC=C(NCC2=CN=C3N=C(N)NC(C3=N2)=O)C=C1)=O

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

RT, protect from light

Solvent & Solubility
In Vitro: 

1M NaOH : 100 mg/mL (226.55 mM; Need ultrasonic)

DMSO : 33.33 mg/mL (75.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2655 mL 11.3276 mL 22.6552 mL
5 mM 0.4531 mL 2.2655 mL 4.5310 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  300 mM Sodium bicarbonate in Water

    Solubility: 50 mg/mL (113.28 mM); Clear solution; Need ultrasonic

  • Protocol 2

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (4.71 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.40%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / 1M NaOH 1 mM 2.2655 mL 11.3276 mL 22.6552 mL 56.6380 mL
5 mM 0.4531 mL 2.2655 mL 4.5310 mL 11.3276 mL
10 mM 0.2266 mL 1.1328 mL 2.2655 mL 5.6638 mL
15 mM 0.1510 mL 0.7552 mL 1.5103 mL 3.7759 mL
20 mM 0.1133 mL 0.5664 mL 1.1328 mL 2.8319 mL
25 mM 0.0906 mL 0.4531 mL 0.9062 mL 2.2655 mL
30 mM 0.0755 mL 0.3776 mL 0.7552 mL 1.8879 mL
40 mM 0.0566 mL 0.2832 mL 0.5664 mL 1.4159 mL
50 mM 0.0453 mL 0.2266 mL 0.4531 mL 1.1328 mL
60 mM 0.0378 mL 0.1888 mL 0.3776 mL 0.9440 mL
1M NaOH 80 mM 0.0283 mL 0.1416 mL 0.2832 mL 0.7080 mL
100 mM 0.0227 mL 0.1133 mL 0.2266 mL 0.5664 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Folic acid
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