2. Others Metabolic Enzyme/Protease
  3. Environmental Pollutants Endogenous Metabolite
  4. Betaine

Betaine  (Synonyms: Trimethylglycine; carboxy-N,N,N-trimethylmethanaminium)

Cat. No.: HY-B0710 Purity: 99.97%
COA
SDS
Handling Instructions Technical Support

Betaine (Trimethylglycine) is a natural compound found in many foods and also an active methyl-donor which can maintain normal DNA methylation patterns[1,2]. Betaine is found ubiquitously in plants, animals, microorganisms, and rich dietary sources including seafood, spinach, and wheat bran. Betaine also acts as an osmolyte, to maintain the avian’s cellular water and ion balance to improve the avian’s capacity against heat stress via preventing dehydration and osmotic inactivation. It helps in maintaining the protective osmolytic activity, especially in heat-stressed birds. Betaine may promote various intestinal microbes against osmotic variations and thus improve microbial fermentation activity.

For research use only. We do not sell to patients.

CAS No. : 107-43-7

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in Water In-stock
Solid
5 g In-stock
10 g In-stock
25 g In-stock
50 g In-stock
100 g In-stock
> 100 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of Betaine:

Betaine Related Antibodies

Top Publications Citing Use of Products

    Betaine purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2025 Apr.

    Betaine (orally at a dose of 400 mg/kg, daily, two months). Western blot representative images display protein expression levels of TGF-β1, Smad2/3, p-Smad2/3, and α-SMA.

    Betaine purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2025 Apr.

    Betaine (orally at a dose of 400 mg/kg, daily, two months). Immunohistochemistry was performed on each group for assessing the expression of Caspase-3.

    Betaine purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2025 Apr.

    Results from cell viability assessments under Betaine (1 mM, one week) in CCSMCs (n = 6).

    Betaine purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2025 Apr.

    Betaine (1 mM, one week). Flow cytometry images and apoptosis rates across CCSMC groups are also depicted.

    Betaine purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2025 Apr.

    Betaine (orally at a dose of 400 mg/kg, daily, two months). Examples of immunofluorescence and relative fluorescence intensities of Bcl-2 (n = 3).

    View All Endogenous Metabolite Isoform Specific Products:

    View All Isoforms
    Human Endogenous Metabolite Microbial Metabolite

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Betaine (Trimethylglycine) is a natural compound found in many foods and also an active methyl-donor which can maintain normal DNA methylation patterns[1,2]. Betaine is found ubiquitously in plants, animals, microorganisms, and rich dietary sources including seafood, spinach, and wheat bran. Betaine also acts as an osmolyte, to maintain the avian’s cellular water and ion balance to improve the avian’s capacity against heat stress via preventing dehydration and osmotic inactivation. It helps in maintaining the protective osmolytic activity, especially in heat-stressed birds. Betaine may promote various intestinal microbes against osmotic variations and thus improve microbial fermentation activity[3].

    IC50 & Target

    Microbial Metabolite

     

    Human Endogenous Metabolite

     

    In Vitro

    Betaine (Trimethylglycine) inhibites proliferation of HeLa cells significantly[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Betaine Related Antibodies
    In Vivo

    Betaine (Trimethylglycine) attenuates liver-to-body weight ratio[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    Molecular Weight

    117.15

    Formula

    C5H11NO2
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    C[N+](C)(C)CC([O-])=O
    Structure Classification
    Initial Source

    Widespread
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    H2O : 100 mg/mL (853.61 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 8.5361 mL 42.6803 mL 85.3606 mL
    5 mM 1.7072 mL 8.5361 mL 17.0721 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.97%

    SDS (396 KB)

    COA (246 KB) HNMR (108 KB) RP-HPLC (217 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [1]

    Cytotoxic effects of Betaine are evaluated by MTT assays. Briefly, 5×103 HeLa or MCF-10A cells/well are seeded in a 96-well plate and cultured overnight. Then, different concentrations of Betaine (from 0 to 100 mg/mL) are added to each well, and the MTT assay is performed at 24, 48, 72 and 96 h after incubation. Cu/Zn-SOD activities are detected using the superoxide dismutase Assay Kit according to the manufacturer’s instructions[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [2]

    Male C57BL/6 mice weighing 25±0.5 g are used in this study. Animals are randomly assigned to four groups (n=6 to 8 per group) and pair-fed a modified Lieber-DeCarli alcohol (AF) or isocaloric maltose dextrin control liquid diet (PF) for 5 weeks with a stepwise feeding procedure. The ethanol content starts at 30% of total calories and gradually increases up to 36% in the last week. Betaine is administered as a supplement in the liquid diets (both PF and AF) at a concentration of 0.5% (w/v), simultaneously with the alcohol. All animals have access to diet and water ad libitum. At the end of the experiment, the mice are killed after being deprived of food for 4 h, and the plasma, liver and epididymal fat pad samples are harvested for assays[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 8.5361 mL 42.6803 mL 85.3606 mL 213.4016 mL
    5 mM 1.7072 mL 8.5361 mL 17.0721 mL 42.6803 mL
    10 mM 0.8536 mL 4.2680 mL 8.5361 mL 21.3402 mL
    15 mM 0.5691 mL 2.8454 mL 5.6907 mL 14.2268 mL
    20 mM 0.4268 mL 2.1340 mL 4.2680 mL 10.6701 mL
    25 mM 0.3414 mL 1.7072 mL 3.4144 mL 8.5361 mL
    30 mM 0.2845 mL 1.4227 mL 2.8454 mL 7.1134 mL
    40 mM 0.2134 mL 1.0670 mL 2.1340 mL 5.3350 mL
    50 mM 0.1707 mL 0.8536 mL 1.7072 mL 4.2680 mL
    60 mM 0.1423 mL 0.7113 mL 1.4227 mL 3.5567 mL
    80 mM 0.1067 mL 0.5335 mL 1.0670 mL 2.6675 mL
    100 mM 0.0854 mL 0.4268 mL 0.8536 mL 2.1340 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Betaine Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Betaine107-43-7Trimethylglycine carboxy-N,N,N-trimethylmethanaminiumEnvironmental PollutantsEndogenous MetaboliteEnvironmental ContaminantsInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Requested Quantity *

    Applicant Name *

    		 

    Salutation

    Email Address *

    		 

    Phone Number *

    Department

    		 

    Organization Name *

    City

    State

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Betaine
    Cat. No.:
    HY-B0710
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    SAFETY DATA SHEET (SDS)

    English - EN (396 KB) Français - FR (396 KB) Deutsch - DE (396 KB) Norwegian - NO (396 KB) Español - ES (396 KB) Swedish - SV (396 KB) Italian - IT (396 KB) Korean - KR (396 KB) Portuguese - PT (396 KB)

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.