1. Kits MCE
  2. Protein Biology
  3. Protein Purification
  4. Agarose Beads
  5. Ni-NTA 6FF Prepacked Column

Ni-NTA 6FF Prepacked Column 

Cat. No.: HY-K0220
Manual COA SDS Technical Support

MCE Ni-NTA 6FF Prepacked Column is ideal for high performance purification of polyhistidine-tagged proteins expressed in E.coli, yeast, insect and mammalian expression systems.

Size Prix Stock Quantité
1 mL En stock
5 mL En stock

* Veuillez sélectionner la quantité avant d'ajouter des articles.

1 Publications Citing Use of MCE Ni-NTA 6FF Prepacked Column

  • Description

  • Stockage

  • Composants

  • Documentation

Description
& Advantages

Ni-NTA His-Tag Purification Agarose, a 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites, enables high-yield, high-purity purification of polyhistidine-tagged proteins. Ni-NTA His-Tag Purification Agarose has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of chemicals and pH values. This makes Ni-NTA 6FF Prepacked Column ideal for high performance purification of polyhistidine-tagged proteins expressed in E.coli, yeast, insect and mammalian expression systems.

Characteristics
Composition 6% highly cross-linked agarose
Bead Diameter 45-165 μm
Binding Capacity > 40 mg 6× His-tagged Protein/mL
Maximum Pressure 0.3 MPa, 3 bar
Storage Solution 50% slurry in a 20% ethanol solution
Stockage

4°C, 2 years.

Composants
Cat. No. Product Name Package
HY-K0220-1 mL Ni-NTA 6FF Prepacked Column 1 mL
HY-K0220-5 mL Ni-NTA 6FF Prepacked Column 5 mL
Documentation

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Vos produits récemment consultés:

Bulk Inquiry

Your information is safe with us. * Required Fields.

Nom du produit

 

Requested Quantity *

Nom du demandeur *

 

Civilité

Adresse e-mail *

Numéro de téléphone *

Department

 

Nom de l'organisation *

City

État

Country or Region *

     

Remarques

Bulk Inquiry

Inquiry Information

Nom du produit:
Ni-NTA 6FF Prepacked Column
Cat. No.:
HY-K0220
Quantité:
MCE Japan Authorized Agent: