Protein G Agarose

製品番号: HY-K0214: Manual COA SDS
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MCE Protein G Agarose, a 4% highly cross-linked agarose reagent coupled with recombinant Protein G, effectively purifies mammalian monoclonal and polyclonal antibodies, such as human IgG3 and rat IgG2a.

容量	価格（税別)	在庫状況	数量
無料サンプル (500 μL)		今すぐ申し込む
5 mL	$230	在庫あり	Estimated Time of Arrival: December 31
10 mL	$380	在庫あり	Estimated Time of Arrival: December 31
50 mL	$1735	在庫あり	Estimated Time of Arrival: December 31

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* アイテムを追加する前、数量をご選択ください

顧客検証

Gel Electrophoresis

: Protein G Agarose purchased from MCE. Usage Cited in: Adv Sci (Weinh). 2025 Nov 30:e11683. [Abstract]; ITM2B truncation or its point mutants and TSPAN4 were expressed in 786‐O cells, their interactions were detected. Protein G Agarose was used in this study (3 h).

: Protein G Agarose purchased from MCE. Usage Cited in: Int J Biol Macromol. 2025 Sep 9;328(Pt 1):147576. [Abstract]; DPV-BAC-CHv50 and DPV-BAC-UL13_C3⁎HA were used to infect DEFs, and silver staining was performed 36 h later to detect specific bands. Protein G Agarose (HY-K0214, 2 h) was used in this experiment.

: Protein G Agarose purchased from MCE. Usage Cited in: Int J Biol Macromol. 2025 Oct 16;331(Pt 2):148358. [Abstract]; Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) analysis of purified monoclonal antibodies. The results demonstrated that all seven mAbs were purified from mouse ascites via protein G affinity chromatography, yielding highly pure antibodies. In this experiment, Protein G Agarose (HY-K0214) was used for the purification of mouse ascites.

: Protein G Agarose purchased from MCE. Usage Cited in: Int J Biol Macromol. 2025 Sep 9;328(Pt 1):147576. [Abstract]; pCAGGS-IFIT5-Flag and pCAGGS-UL13-HA were transfected into duck embryo fibroblasts, and the interaction between IFIT5-Flag and UL13-HA was verified by co-immunoprecipitation. Protein G Agarose (HY-K0214, 2 h) was used in this experiment.

: Protein G Agarose purchased from MCE. Usage Cited in: Int Immunopharmacol. 2025 May 23:159:114913. [Abstract]; Immunoprecipitation (IP) was performed using a PKCα or a EP300 antibody to observe the interaction of PKCα and EP300. PKCα and EP300 protein levels in the lysates of HCECs were detected using WB. Protein G Agarose (HY-K0214) was used in this experiment.

説明
保管条件
成分
ドキュメンテーション

Description
& Advantages

Protein G is a bacterial cell wall protein isolated from group G Streptococci and binds to most mammalian IgGs mainly through Fc regions. Native Protein G contains 3 IgG binding domains and sites for albumin and cell surface binding. The latter have been eliminated from recombinant Protein G to reduce nonspecific binding.

Characteristics
Composition	4% cross-linked agarose
Degree of Substitution	2 mg Recombinant Protein G/mL Settled Resin
Bead Diameter	45-165 μm
Binding Capacity	20 mg Human IgG/mL Settled Resin
Storage Solution	50% slurry in 1× PBS containing 20% ethanol

The specifications of the product correspond to the actual resin volume, with the resin content of 50%.

保管条件

Store at 2-8°C, and is stable for at least 2 years.

Do not dry or freeze.

成分

Components	HY-K0214-5 mL	HY-K0214-10 mL	HY-K0214-50 mL
Protein G Agarose (Settled Resin)	5 mL	10 mL	50 mL

ドキュメンテーション

Manual (307 KB) SDS (251 KB)

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Protein G affinity resin

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Protein G Agarose

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