2. MCE Kits
  3. Protein Biology
  4. Protein Purification
  5. Agarose Beads
  6. Protein G Agarose

Protein G Agarose 

Cat. No.: HY-K0214
Manual COA SDS Technical Support
Magnetic Beads

MCE Protein G Agarose, a 4% highly cross-linked agarose reagent coupled with recombinant Protein G, effectively purifies mammalian monoclonal and polyclonal antibodies, such as human IgG3 and rat IgG2a.

사이즈 가격 재고 수량
무료 샘플 (500 μL)   지금 신청하기  
5 mL 해외재고보유
10 mL 해외재고보유
50 mL 해외재고보유
or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

    Protein G Agarose purchased from MCE. Usage Cited in: Adv Sci (Weinh). 2025 Nov 30:e11683.  [Abstract]

    ITM2B truncation or its point mutants and TSPAN4 were expressed in 786‐O cells, their interactions were detected. Protein G Agarose was used in this study (3 h).

    Protein G Agarose purchased from MCE. Usage Cited in: Int J Biol Macromol. 2025 Sep 9;328(Pt 1):147576.  [Abstract]

    DPV-BAC-CHv50 and DPV-BAC-UL13C3⁎HA were used to infect DEFs, and silver staining was performed 36 h later to detect specific bands. Protein G Agarose (HY-K0214, 2 h) was used in this experiment.

    Protein G Agarose purchased from MCE. Usage Cited in: Int J Biol Macromol. 2025 Oct 16;331(Pt 2):148358.  [Abstract]

    Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) analysis of purified monoclonal antibodies. The results demonstrated that all seven mAbs were purified from mouse ascites via protein G affinity chromatography, yielding highly pure antibodies. In this experiment, Protein G Agarose (HY-K0214) was used for the purification of mouse ascites.

    Protein G Agarose purchased from MCE. Usage Cited in: Int J Biol Macromol. 2025 Sep 9;328(Pt 1):147576.  [Abstract]

    pCAGGS-IFIT5-Flag and pCAGGS-UL13-HA were transfected into duck embryo fibroblasts, and the interaction between IFIT5-Flag and UL13-HA was verified by co-immunoprecipitation. Protein G Agarose (HY-K0214, 2 h) was used in this experiment.

    Protein G Agarose purchased from MCE. Usage Cited in: Int Immunopharmacol. 2025 May 23:159:114913.  [Abstract]

    Immunoprecipitation (IP) was performed using a PKCα or a EP300 antibody to observe the interaction of PKCα and EP300. PKCα and EP300 protein levels in the lysates of HCECs were detected using WB. Protein G Agarose (HY-K0214) was used in this experiment.

    • 제품 설명

    • 보관

    • 구성요소

    • 각종 서류

    Description
    & Advantages

    Protein G is a bacterial cell wall protein isolated from group G Streptococci and binds to most mammalian IgGs mainly through Fc regions. Native Protein G contains 3 IgG binding domains and sites for albumin and cell surface binding. The latter have been eliminated from recombinant Protein G to reduce nonspecific binding.


    MCE Protein G Agarose, a 4% highly cross-linked agarose reagent coupled with recombinant Protein G, effectively purifies mammalian monoclonal and polyclonal antibodies, such as human IgG3 and rat IgG2a.


    Characteristics
    Composition 4% cross-linked agarose
    Degree of Substitution 2 mg Recombinant Protein G/mL Settled Resin
    Bead Diameter 45-165 μm
    Binding Capacity 20 mg Human IgG/mL Settled Resin
    Storage Solution 50% slurry in 1× PBS containing 20% ethanol

     

    The specifications of the product correspond to the actual resin volume, with the resin content of 50%.
    보관

    Store at 2-8°C, and is stable for at least 2 years.


    Do not dry or freeze.
    구성요소
    Components HY-K0214-5 mL HY-K0214-10 mL HY-K0214-50 mL
    Protein G Agarose (Settled Resin) 5 mL 10 mL 50 mL
    각종 서류

    Manual (307 KB) SDS (251 KB)
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Protein G affinity resin

    최근 본 상품:

    대량구매 문의

    Your information is safe with us. * Required Fields.

    상품명

    		  

    Requested Quantity *

    고객명 *

    		 

    호칭

    메일주소 *

    전화번호 *

    Department

    		 

    회사명 *

    City

    도

    Country or Region *

    		      

    비고

    대량구매 문의

    Inquiry Information

    상품명:
    Protein G Agarose
    Cat. No.:
    HY-K0214
    수량:
    MCE Japan Authorized Agent:

    Request for COA Report

    Please fill out this form to request the COA report. We will send it to your email within 3 working days. Your information is safe with us. * Required Fields.

    .

    Product Name

    		  

    Lot # *

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Remarks

    Request for COA Report

    We have received your request and will respond to you as soon as possible.