1. MCE Kits
  2. Protein Biology
  3. Protein Purification
  4. Agarose Beads
  5. Streptavidin Agarose 6FF

Streptavidin Agarose 6FF 

Cat. No.: HY-K0218A
Manual COA SDS Technical Support

MCE Streptavidin Agarose 6FF, a 6% highly cross-linked agarose reagent coupled with recombinant streptavidin, is an affinity chromatography medium for separation and purification of biotinylated peptides, antibodies, lectins, etc. The total binding capacity of Streptavidin Agarose 6FF is more than 200 nmol of D-Biotin/mL settled resin.

사이즈 가격 재고 수량
무료 샘플 (1 mL)   지금 신청하기  
5 mL 해외재고보유
10 mL 해외재고보유
50 mL 해외재고보유

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

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Description
& Advantages

Streptavidin, isolated from Streptomyces avidinii, binds 4 moles of biotin per mole of protein with an extremely high affinity. With no carbohydrate group and an isoelectric point of 6.5 (different from avidin), streptavidin has less nonspecific binding. Streptavidin has a strong affinity with biotin and needs to be eluted under denaturing conditions when purified. However, streptavidin has a relatively weak affinity for iminobiotin, which binds at pH 9.5-11.0 and elutes at pH 4.0 without the use of denaturing agents.


Characteristics
Composition 6% cross-linked agarose
Ligand Streptavidin
Bead Diameter > 45-165 μm
Binding Capacity > 200 nmol of D-Biotin/mL Settled Resin
Maximum Pressure 0.3 MPa, 3 bar
pH stability range 4.0-9.0

 

The specifications of the product correspond to the actual resin volume, with the resin content of 50%.

보관

2-8°C, 2 years.


Do not dry or freeze.

구성요소
Components HY-K0218A-5 mL HY-K0218A-10 mL HY-K0218A-50 mL
Streptavidin Agarose 6FF 5 mL 10 mL 50 mL
각종 서류

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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상품명:
Streptavidin Agarose 6FF
Cat. No.:
HY-K0218A
수량:
MCE Japan Authorized Agent: