2. MCE-Kits
  3. Protein Biology
  4. Protein Purification
  5. Agarose Beads
  6. Streptavidin Agarose 6FF

Streptavidin Agarose 6FF 

Art. -Nr.: HY-K0218A
Manual COA SDS Technical Support
Magnetic Beads

MCE Streptavidin Agarose 6FF, a 6% highly cross-linked agarose reagent coupled with recombinant streptavidin, is an affinity chromatography medium for separation and purification of biotinylated peptides, antibodies, lectins, etc. The total binding capacity of Streptavidin Agarose 6FF is more than 200 nmol of D-Biotin/mL settled resin.

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Kostenlose Probe (1 mL)   Jetzt bewerben  
5 mL Auf Lager
10 mL Auf Lager
50 mL Auf Lager
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  • Beschreibung

  • Speicherung

  • Komponenten

  • Dokumentation

Description
& Advantages

Streptavidin, isolated from Streptomyces avidinii, binds 4 moles of biotin per mole of protein with an extremely high affinity. With no carbohydrate group and an isoelectric point of 6.5 (different from avidin), streptavidin has less nonspecific binding. Streptavidin has a strong affinity with biotin and needs to be eluted under denaturing conditions when purified. However, streptavidin has a relatively weak affinity for iminobiotin, which binds at pH 9.5-11.0 and elutes at pH 4.0 without the use of denaturing agents.


Characteristics
Composition 6% cross-linked agarose
Ligand Streptavidin
Bead Diameter > 45-165 μm
Binding Capacity > 200 nmol of D-Biotin/mL Settled Resin
Maximum Pressure 0.3 MPa, 3 bar
pH stability range 4.0-9.0

 

The specifications of the product correspond to the actual resin volume, with the resin content of 50%.
Speicherung

2-8°C, 2 years.


Do not dry or freeze.
Komponenten
Components HY-K0218A-5 mL HY-K0218A-10 mL HY-K0218A-50 mL
Streptavidin Agarose 6FF 5 mL 10 mL 50 mL
Dokumentation

Manual (267 KB) SDS (130 KB)
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Streptavidin gelStreptavidin-biotin gelSA agarose gel

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