Isorhamnetin-3-O-neohespeidoside

Name: Isorhamnetin-3-O-neohespeidoside
Brand: MedChemExpress
SKU: HY-N0778
Rating: 5.0 (3 reviews)

Cat. No.: HY-N0778 Purity: 99.93%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Isorhamnetin-3-O-neohespeidoside is a flavonoid. Isorhamnetin-3-O-neohespeidoside can be isolated from Typha angustifolia. Isorhamnetin-3-O-neohespeidoside inhibits xanthine oxidase activity with an IC₅₀ of 48.75 μg mL. Isorhamnetin-3-O-neohespeidoside has antioxidant and osteoclastogenic activities. Isorhamnetin-3-O-neohespeidoside can be used in research of bone.

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Isorhamnetin-3-O-neohespeidoside 화학구조

CAS No. : 55033-90-4

사이즈	재고	수량
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid
1 mg	해외재고보유	Estimated Time of Arrival: December 31
5 mg	해외재고보유	Estimated Time of Arrival: December 31
10 mg	해외재고보유	Estimated Time of Arrival: December 31
25 mg	해외재고보유	Estimated Time of Arrival: December 31
50 mg	해외재고보유	Estimated Time of Arrival: December 31
100 mg	해외재고보유	Estimated Time of Arrival: December 31
200 mg	견적 받기
500 mg	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 3 publication(s) in Google Scholar

Other Forms of Isorhamnetin-3-O-neohespeidoside:

Isorhamnetin-3-O-neohespeidoside (Standard) 견적 받기

3 Publications Citing Use of MCE Isorhamnetin-3-O-neohespeidoside

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•Related Screening Libraries:

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Biological Activity
순도&문서
References
고객리뷰

제품 설명

IC₅₀ & Target

xanthine oxidase: IC₅₀ of 48.75 μg mL

Cellular Effect

Cell Line	Type	Value	Description	References
Bel-7402	IC₅₀	74.4 μM Compound: 2	Cytotoxicity against human Bel7402 cells Cytotoxicity against human Bel7402 cells	10.1007/s00044-013-0773-3
Caco-2	IC₅₀	2.47 μg/mL Compound: 2	Cytotoxicity against human Caco2 cells after 48 hrs by MTT assay Cytotoxicity against human Caco2 cells after 48 hrs by MTT assay	10.1007/s00044-013-0773-3
HCT-116	IC₅₀	72 μM Compound: 2	Cytotoxicity against human HCT116 cells Cytotoxicity against human HCT116 cells	10.1007/s00044-013-0773-3
NIH3T3	IC₅₀	>200 μg/mL Compound: 2	Cytotoxicity against mouse NIH/3T3 cells after 96 hrs by MTT assay Cytotoxicity against mouse NIH/3T3 cells after 96 hrs by MTT assay	10.1007/s00044-013-0773-3
Raji	IC₅₀	574 molar ratio Compound: 11	Inhibition of TPA-induced EBV-early antigen activation in human Raji cells relative to TPA Inhibition of TPA-induced EBV-early antigen activation in human Raji cells relative to TPA	[PMID: 17190444]
T47D	IC₅₀	0.003 μg/mL Compound: 2	Cytotoxicity against human T47D cells after 48 hrs by MTT assay Cytotoxicity against human T47D cells after 48 hrs by MTT assay	10.1007/s00044-013-0773-3

In Vitro

Isorhamnetin-3-O-neohespeidoside (greater than 0.025 mg/mL) scavenges ABTS^+• free radicals in a concentration-dependent manner with an IC₅₀ of 0.06 mg/mL^[2].
Isorhamnetin-3-O-neohespeidoside (19-150 μg/mL) inhibits xanthine oxidase activity with an IC₅₀ of 48.75 μg mL and scavenges superoxide anions with an IC₅₀ of 30 μg mL^[2].
Isorhamnetin-3-O-neohespeidoside (1-50 μM; 4 days) promotes RANKL-induced osteoclastogenesis in BMMs in a dose-dependent manner, increasing the number and area of TRAP-positive osteoclasts^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Isorhamnetin-3-O-neohespeidoside (18.75 mg/kg; gingival injection; 4 days) promotes resorption of crown-covered bone, increases the number of TRAP-positive osteoclasts, and upregulates RANKL protein expression in the crown coverage of dental follicle in C57BL/6 mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

분자량

624.54

화학식

C₂₈H₃₂O₁₆

CAS No.

55033-90-4

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1C(O[C@H](O[C@H](CO)[C@@H](O)[C@@H]2O)[C@@H]2O[C@@](O[C@@H](C)[C@H](O)[C@H]3O)([H])[C@@H]3O)=C(C4=CC(OC)=C(O)C=C4)OC5=CC(O)=CC(O)=C15

Structure Classification

Initial Source

선적

Room temperature in continental US; may vary elsewhere.

보관

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

용액&용해도

In Vitro:

DMSO : 250 mg/mL (400.29 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.6012 mL	8.0059 mL	16.0118 mL
5 mM	0.3202 mL	1.6012 mL	3.2024 mL
10 mM	0.1601 mL	0.8006 mL	1.6012 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.33 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (3.33 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (3.33 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

순도&문서

Purity: 99.93%

Select Batch:

Data Sheet (280 KB) SDS (393 KB)

COA (257 KB) HNMR (282 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Wang X, et al. A rapid and efficient extraction method based on industrial MCM-41-miniaturized matrix solid-phase dispersion extraction with response surface methodology for simultaneous quantification of six flavonoids in Pollen typhae by ultra-high-performance liquid chromatography. J Sep Sci. 2019 Jul;42(14):2426-2434. [Content Brief]

[2]. Bouhlel I, et al. Assessment of isorhamnetin 3-O-neohesperidoside from Acacia salicina: protective effects toward oxidation damage and genotoxicity induced by aflatoxin B1 and nifuroxazide. J Appl Toxicol. 2010 Aug;30(6):551-8. [Content Brief]

[3]. Yu X, et al. Isorhamnetin 3-O-neohesperidoside promotes the resorption of crown-covered bone during tooth eruption by osteoclastogenesis. Sci Rep. 2020 Mar 20;10(1):5172. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.6012 mL	8.0059 mL	16.0118 mL	40.0295 mL
	5 mM	0.3202 mL	1.6012 mL	3.2024 mL	8.0059 mL
	10 mM	0.1601 mL	0.8006 mL	1.6012 mL	4.0029 mL
	15 mM	0.1067 mL	0.5337 mL	1.0675 mL	2.6686 mL
	20 mM	0.0801 mL	0.4003 mL	0.8006 mL	2.0015 mL
	25 mM	0.0640 mL	0.3202 mL	0.6405 mL	1.6012 mL
	30 mM	0.0534 mL	0.2669 mL	0.5337 mL	1.3343 mL
	40 mM	0.0400 mL	0.2001 mL	0.4003 mL	1.0007 mL
	50 mM	0.0320 mL	0.1601 mL	0.3202 mL	0.8006 mL
	60 mM	0.0267 mL	0.1334 mL	0.2669 mL	0.6672 mL
	80 mM	0.0200 mL	0.1001 mL	0.2001 mL	0.5004 mL
	100 mM	0.0160 mL	0.0801 mL	0.1601 mL	0.4003 mL