2. Metabolic Enzyme/Protease
  3. Glutaminase
  4. L-Methionine-DL-sulfoximine

L-Methionine-DL-sulfoximine  (Synonyms: MSX; MSO)

Cat. No.: HY-B1692 Pureté: 98.0%
COA
SDS
Instruction de manipulation Technical Support

L-Methionine-DL-sulfoximine (MSX; MSO), a highly specific and irreversible inhibitor of Glutamine synthetase (GS), is also a potent convulsant which metabolically and morphologically primarily affects astroglia. L-Methionine-DL-sulfoximine has been employed to inhibit the Gln-dependent ammonia-stimulated neuronal toxicity in vitro, potentiating Gln deficit-dependent depression. L-Methionine-DL-sulfoximine tremendously increases the rate of release of fixed nitrogen in cyanobacteria. L-Methionine-DL-sulfoximine is a promising candidate for research in biofertilizers and convulsive seizures (CS).

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CAS No. : 15985-39-4

Size Prix Stock Quantité
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 En stock
Solution
10 mM * 1 mL in Water En stock
Solid
100 mg En stock
500 mg En stock
1 g En stock
5 g En stock
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50 g   Obtenir un devis  

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Based on 6 publication(s) in Google Scholar

L-Methionine-DL-sulfoximine Related Antibodies

Top Publications Citing Use of Products

    L-Methionine-DL-sulfoximine purchased from MedChemExpress. Usage Cited in: J Agric Food Chem. 2024 Jan 10;72(1):449-460.  [Abstract]

    MSO (20 mg/kg body weight, intraperitoneal injection twice a week for 14 weeks). H&E staining of iWAT and eWAT sections.

    L-Methionine-DL-sulfoximine purchased from MedChemExpress. Usage Cited in: J Agric Food Chem. 2024 Jan 10;72(1):449-460.  [Abstract]

    MSO (20 mg/kg body weight, intraperitoneal injection twice a week for 14 weeks). Weight of adipose tissues (iWAT, eWAT, and BAT) in mice (n = 7).

    L-Methionine-DL-sulfoximine purchased from MedChemExpress. Usage Cited in: J Agric Food Chem. 2024 Jan 10;72(1):449-460.  [Abstract]

    MSO (20 mg/kg body weight, intraperitoneal injection twice a week for 14 weeks). Ratio of fat mass to body weight (n = 7).

    L-Methionine-DL-sulfoximine purchased from MedChemExpress. Usage Cited in: J Agric Food Chem. 2024 Jan 10;72(1):449-460.  [Abstract]

    MSO (20 mg/kg body weight, intraperitoneal injection twice a week for 14 weeks). The content of TG in serum.

    L-Methionine-DL-sulfoximine purchased from MedChemExpress. Usage Cited in: J Agric Food Chem. 2024 Jan 10;72(1):449-460.  [Abstract]

    MSO (20 mg/kg body weight, intraperitoneal injection twice a week for 14 weeks). H&E staining images of liver sections. Scale bar = 200 μm.

    • Activité biologique

    • Pureté et documentation

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    Description

    L-Methionine-DL-sulfoximine (MSX; MSO), a highly specific and irreversible inhibitor of Glutamine synthetase (GS), is also a potent convulsant which metabolically and morphologically primarily affects astroglia. L-Methionine-DL-sulfoximine has been employed to inhibit the Gln-dependent ammonia-stimulated neuronal toxicity in vitro, potentiating Gln deficit-dependent depression. L-Methionine-DL-sulfoximine tremendously increases the rate of release of fixed nitrogen in cyanobacteria. L-Methionine-DL-sulfoximine is a promising candidate for research in biofertilizers and convulsive seizures (CS)[1][2][3][4][5].

    In Vivo

    L-Methionine-DL-sulfoximine (75 mg/kg, i.p., 210 min) exerts no affect on the hippocampal Gutamine synthetase (GS) activity and the expression of GS protein for the first 150 min, but significantly decreases the activity of GS during 165-210 min in rats. Additionally, L-Methionine-DL-sulfoximine also reduces the intensity of convulsive seizures (CS) caused by Pilocarpine (Pilo) (HY-B0726A) administration and seizure in rats[1].
    L-Methionine-DL-sulfoximine (75 mg/kg, i.p.) reduces D-Asp release evoked by K+-induced depolarization and unchanges Glu uptake in the hippocampus and enthorhinal cortex of temporal lobe epilepsy (TLE) model rats[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male rats (24 days old, Sprague Dawley)[1]
    Dosage: 75 mg/kg
    Administration: 210 min, i.p., measurements were taken at MSO “0”, after 15 min (referred to as MSO “15”, Pilo “15” and MSO + Pilo “15”) and after 60 min (MSO “60”, Pilo “60” and MSO + Pilo “60”, respectively
    Result: Did not affect the hippocampal GS activity and the expression of GS protein for the first 150 min, but significantly decreased the activity during 165-210 min after MSO administration in rats. Additionally, MSO reduced the intensity of CS caused by Pilo administration.
    Animal Model: Hippocampus and enthorhinal cortex of TLE model rats[4]
    Dosage: 75 mg/kg
    Administration: i.p., Administration 150 minutes before decapitation
    Result: Markedly reduced D-Asp release evoked by K+-induced depolarization and slightly decreased [3H]D-Asp uptake at higher concentrations of [3H]D-Asp in TLE model rats.
    Masse moléculaire

    180.23

    Formule

    C5H12N2O3S
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(O)[C@@H](N)CCS(=N)(C)=O
    Livraison

    Room temperature in continental US; may vary elsewhere.
    Stockage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvant et solubilité
    In Vitro: 

    H2O : 50 mg/mL (277.42 mM; Need ultrasonic)

    DMSO : < 1 mg/mL (insoluble or slightly soluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 5.5485 mL 27.7423 mL 55.4847 mL
    5 mM 1.1097 mL 5.5485 mL 11.0969 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Calculateur de molarité

    • Calculateur de dilution

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (554.85 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Pureté et documentation

    Purity: 98.0%

    SDS (393 KB)

    COA (248 KB) HNMR (265 KB) MS (99 KB)

    Instruction de manipulation (2659 KB)
    Références

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 5.5485 mL 27.7423 mL 55.4847 mL 138.7116 mL
    5 mM 1.1097 mL 5.5485 mL 11.0969 mL 27.7423 mL
    10 mM 0.5548 mL 2.7742 mL 5.5485 mL 13.8712 mL
    15 mM 0.3699 mL 1.8495 mL 3.6990 mL 9.2474 mL
    20 mM 0.2774 mL 1.3871 mL 2.7742 mL 6.9356 mL
    25 mM 0.2219 mL 1.1097 mL 2.2194 mL 5.5485 mL
    30 mM 0.1849 mL 0.9247 mL 1.8495 mL 4.6237 mL
    40 mM 0.1387 mL 0.6936 mL 1.3871 mL 3.4678 mL
    50 mM 0.1110 mL 0.5548 mL 1.1097 mL 2.7742 mL
    60 mM 0.0925 mL 0.4624 mL 0.9247 mL 2.3119 mL
    80 mM 0.0694 mL 0.3468 mL 0.6936 mL 1.7339 mL
    100 mM 0.0555 mL 0.2774 mL 0.5548 mL 1.3871 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    L-Methionine-DL-sulfoximine Related Classifications

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    L-Methionine-DL-sulfoximine15985-39-4MSX MSOGlutaminaseglutamine synthetaseconvulsantastrogliadepressionfixed nitrogenbiofertilizersconvulsive seizuresInhibitorinhibitorinhibit

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