2. Metabolic Enzyme/Protease
  3. Drug Metabolite Endogenous Metabolite
  4. NAPQI

NAPQI  (Synonyms: N-Acetyl-4-benzoquinone Imine)

Cat. No.: HY-W017464 Pureza: 98.0%
COA
SDS
Instrucciones de manejo Technical Support

NAPQI is the toxic metabolite of Acetaminophen (HY-66005). NAPQI is also an inhibitor of enzymes in the vitamin K cycle. NAPQI is rapidly detoxified by glutathione (GSH), but in situations of GSH deficiency, excess NAPQI reacts with cysteine residues in proteins, causing cell death and toxicity in the liver.

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NAPQI

NAPQI Estructura química

No. CAS : 50700-49-7

Tamaño Precio Stock Cantidad
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 En stock
Solution
10 mM * 1 mL in DMSO En stock
Solid
1 mg En stock
5 mg En stock
10 mg En stock
50 mg   Obtener un presupuesto  
100 mg   Obtener un presupuesto  

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Other Forms of NAPQI:

NAPQI Related Antibodies

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Human Endogenous Metabolite Microbial Metabolite

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Descripciòn

NAPQI is the toxic metabolite of Acetaminophen (HY-66005). NAPQI is also an inhibitor of enzymes in the vitamin K cycle. NAPQI is rapidly detoxified by glutathione (GSH), but in situations of GSH deficiency, excess NAPQI reacts with cysteine residues in proteins, causing cell death and toxicity in the liver[1][2].

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

NAPQI (25-400 μM, 30 min) inhibits the synthesis of glutathione synthase (GS)[3].
NAPQI (0.15 mg/mL, 24-48 h) is toxic to auditory cell HEI-OC1 through oxidation and endoplasmic reticulum stress-dependent pathways[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

NAPQI Related Antibodies

Cell Viability Assay[4]

Cell Line: HEI-OC1
Concentration: 0.15 mg/mL
Incubation Time: 24, 48 h
Result: Decreased viability of HEI-OC1 cells at 24 and 48 h.
Peso molecular

149.15

Fòrmula

C8H7NO2
No. CAS
Appearance

Solid

Color

Light yellow to yellow

SMILES

CC(/N=C1C=CC(C=C\1)=O)=O
Structure Classification
Initial Source
Envío

Shipping with dry ice.
Almacenamiento

-80°C, protect from light, stored under nitrogen
Solvente y solubilidad
In Vitro: 

DMSO : 100 mg/mL (670.47 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.7047 mL 33.5233 mL 67.0466 mL
5 mM 1.3409 mL 6.7047 mL 13.4093 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months.

  • Calculadora de molaridad

  • Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Pureza y Documentación

Purity: 99.33%

SDS (393 KB)

COA (247 KB) HNMR (137 KB) RP-HPLC (168 KB) LCMS (224 KB) Elemental Analysis Report (106 KB)

Instrucciones de manejo (2659 KB)
Referencias

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 6.7047 mL 33.5233 mL 67.0466 mL 167.6165 mL
5 mM 1.3409 mL 6.7047 mL 13.4093 mL 33.5233 mL
10 mM 0.6705 mL 3.3523 mL 6.7047 mL 16.7617 mL
15 mM 0.4470 mL 2.2349 mL 4.4698 mL 11.1744 mL
20 mM 0.3352 mL 1.6762 mL 3.3523 mL 8.3808 mL
25 mM 0.2682 mL 1.3409 mL 2.6819 mL 6.7047 mL
30 mM 0.2235 mL 1.1174 mL 2.2349 mL 5.5872 mL
40 mM 0.1676 mL 0.8381 mL 1.6762 mL 4.1904 mL
50 mM 0.1341 mL 0.6705 mL 1.3409 mL 3.3523 mL
60 mM 0.1117 mL 0.5587 mL 1.1174 mL 2.7936 mL
80 mM 0.0838 mL 0.4190 mL 0.8381 mL 2.0952 mL
100 mM 0.0670 mL 0.3352 mL 0.6705 mL 1.6762 mL
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NAPQI Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NAPQI50700-49-7N-Acetyl-4-benzoquinone ImineDrug MetaboliteEndogenous Metabolitetoxic metabolitevitamin K cyclehepatotoxicityInhibitorinhibitorinhibit

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Nombre del producto:
NAPQI
Cat. No.:
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SAFETY DATA SHEET (SDS)

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