2. Immunology/Inflammation Apoptosis
  3. Toll-like Receptor (TLR) Apoptosis NO Synthase
  4. ODN 1826

ODN 1826  (Synonyms: CpG 1826)

製品番号: HY-146245 純度: 99.51%
COA 取扱説明書 Technical Support

ODN 1826 is a class B CpG ODN (oligodeoxynucleotide) and TLR9 agonist. ODN 1826 induces NO and iNOS production and enhances Apoptosis. ODN 1826 enhances immune surveillance. ODN 1826 increases aortic atherosclerotic plaque size. ODN 1826 has antitumor activity against lung cancer, glioma and melanoma.

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CAS 番号 : 202668-42-6

容量 価格（税別） 在庫状況 数量
1 mg $264 在庫あり
5 mg $630 在庫あり
10 mg $1023 在庫あり
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100 mg   お問い合わせ  

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カスタマーレビュー

Based on 10 publication(s) in Google Scholar

Other Forms of ODN 1826:

ODN 1826 関連抗体

Top Publications Citing Use of Products

    ODN 1826 purchased from MedChemExpress. Usage Cited in: Adv Mater. 2025 Sep 1:e02375.  [Abstract]

    ODN 1826 (CpG, 500 ng per well; 24 h) significantly promoted the repolarization of M2-typed bone marrow-derived cells (BMDM), which stood for tumor-associated macrophages (TAM), with a significant increase of M1 (iNOS) and a decrease of M2 markers (Fizz1 and Mrc1).

    ODN 1826 purchased from MedChemExpress. Usage Cited in: Life Sci. 2025 Aug 29:380:123930.  [Abstract]

    Cytotoxicity analysis of DC 2.4 cells treated with DNA-OprF/PcrV + CpG (ODN 1826) for 24 h. CpG was functionally integrated with the DNA vaccine via physical mixing. Specifically, 10 μg of pVAX1-OprF, 10 μg of pVAX1-PcrV, and 10 μg of CpG ODN were vortex-mixed in 150 μl of 1640 medium, followed by the addition of 60 μl of transfection reagent (mass-to-volume ratio = 1:2). The mixture was incubated at room temperature for 30 min to form transfection complexes, sterilized by passage through a 0.22 μm filter, and used immediately.

    ODN 1826 purchased from MedChemExpress. Usage Cited in: Life Sci. 2025 Aug 29:380:123930.  [Abstract]

    ODN 1826 (CpG, 0.1 μg/μL; s.c.; 200 μL injected every 2 weeks for a total of three times).
    HE-stained image of pathological sections of the heart, liver, lung, and kidney after immunization (100×). Scale bar = 50 μm. These results revealed no obvious tissue pathological changes or inflammatory cell infiltration in the four treatment groups, including the DNA-OprF/PcrV + CpG group.

    ODN 1826 purchased from MedChemExpress. Usage Cited in: Neurochem Res. 2025 Sep 5;50(5):288.  [Abstract]

    ODN 1826 (CpG) regulate astrocyte cell viability and TLR9/NF-κB pathway activation. Human astrocytes were treated with varying concentrations of CpG oligodeoxynucleotides (0, 100, 1000, and 5000 ng/mL) for 24 h. Cell viability was assessed using CCK-8 assay.

    ODN 1826 purchased from MedChemExpress. Usage Cited in: Neurochem Res. 2025 Sep 5;50(5):288.  [Abstract]

    ODN 1826 (CpG) regulate astrocyte cell viability and TLR9/NF-κB pathway activation. Human astrocytes were treated with varying concentrations of CpG oligodeoxynucleotides (0, 100, 1000, and 5000 ng/mL) for 24 h. Apoptosis rates were determined by flow cytometry analysis using Annexin V-FITC and propidium iodide staining.

    ODN 1826 purchased from MedChemExpress. Usage Cited in: Neurochem Res. 2025 Sep 5;50(5):288.  [Abstract]

    ODN 1826 (CpG) regulate astrocyte cell viability and TLR9/NF-κB pathway activation. Human astrocytes were treated with varying concentrations of CpG oligodeoxynucleotides (0, 100, 1000, and 5000 ng/mL) for 24 h. Protein expression levels of TLR9, total NF-κB, and phosphorylated NF-κB (p-NF-κB) were analyzed by Western blot with GAPDH as loading control.

    Toll-like Receptor (TLR) アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    TLR3 TLR8 TLR9 TLR2 TLR4 TLR7 TLR1 TLR6

    NO Synthase アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    nNOS iNOS eNOS

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    製品説明

    ODN 1826 is a class B CpG ODN (oligodeoxynucleotide) and TLR9 agonist. ODN 1826 induces NO and iNOS production and enhances Apoptosis. ODN 1826 enhances immune surveillance. ODN 1826 increases aortic atherosclerotic plaque size. ODN 1826 has antitumor activity against lung cancer, glioma and melanoma[1][2][3][4][5][6].

    IC50 & Target

    TLR9

     

    体外実験

    ODN 1826 (1 μg/mL; 24 h) induces nitric oxide (NO) and inducible nitric oxide synthase (iNOS) production in RAW 264.7 mouse macrophages[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    ODN 1826 関連抗体
    体内実験

    CpG-1826 (100 μg/tumor; intratumoral injection; every 3 days for up to 4 times) delays the growth of subcutaneously implanted GL261 gliomas in C57BL/6NTac mice, inducing increased infiltration of macrophages and B cells into tumors, with significant growth delay in ~50% of mice[4]. ODN 1826 (18 nM; s.c.; 3 times a week for 7 weeks) increases aortic atherosclerotic plaque size in a chronic vascular injury mouse model[5].
    ODN 1826 (0.05 mg; i.p.; 1, 3, 5, 8, 11, 13 days) has a strong anti-tumor growth effect in a Lewis lung cancer mouse tumor model[6].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: 6-week-old male C57BL/6NTac mice; GL261 glioma or B16F10 melanoma subcutaneously implanted in posterior flanks[4].
    Dosage: 100 μg/tumor
    Administration: Intratumoral injection; every 3 days for up to 4 treatments
    Result: Slowed tumor growth, increased F4/80+ macrophages and B220+ B cells in tumors, with variable responses (50% mice showed prolonged stasis) (in GL261 glioma model).
    Achieved complete tumor regression and induced long-term immune memory in combination with Cyclophosphamide (HY-17420).
    Delayed growth but did not induce regression, with modest increases in F4/80+ macrophages and B220+ B cells in combination with Cyclophosphamide (in B16F10 melanoma model).
    分子量

    6364.10

    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    Sequence

    DNA, d(P-thio)(T-C-C-A-T-G-A-C-G-T-T-C-C-T-G-A-C-G-T-T)
    SMILES

    [ODN 1826]
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    -20°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
    溶剤 & 溶解度
    体外: 

    H2O : 50 mg/mL (7.86 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.1571 mL 0.7857 mL 1.5713 mL
    5 mM 0.0314 mL 0.1571 mL 0.3143 mL
    10 mM --- --- ---
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    一般には略語で表示されます：C1V1 = C2V2

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    純度とドキュメンテーション

    純度: 99.69%

    COA (247 KB) LCMS (319 KB)

    取扱説明書 (2242 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 0.1571 mL 0.7857 mL 1.5713 mL 3.9283 mL
    5 mM 0.0314 mL 0.1571 mL 0.3143 mL 0.7857 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    ODN 1826 Related Classifications

    • Molarity Calculator

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    質量   濃度   体積   分子量 *
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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    ODN 1826202668-42-6CpG 1826ODN1826ODN-1826CpG1826CpG-1826Toll-like Receptor (TLR)ApoptosisNO SynthaseNitric oxide synthasesNOSCPG ODNTLRAgonistAntitumorRAW 264.7Lewis Lung Cancer Murine Tumor ModelInhibitorinhibitorinhibit

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