2. Neuronal Signaling Metabolic Enzyme/Protease
  3. OGT Acyltransferase
  4. OSMI-1

OSMI-1 is a cell-permeable O-GlcNAc transferase (OGT) inhibitor with an IC50 value of 2.7 μM. OSMI-1 inhibits protein O-linked N-acetylglucosamine (O-GlcNAcylation) in several mammalian cell lines without qualitatively altering cell surface N- or O-linked glycans.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 1681056-61-0

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 123 在庫あり
Solution
10 mM * 1 mL in DMSO USD 123 在庫あり
Solid
5 mg $99 在庫あり
10 mg $165 在庫あり
25 mg $275 在庫あり
50 mg $495 在庫あり
100 mg $675 在庫あり
500 mg $2025 在庫あり
1 g $3000 在庫あり
5 g   お問い合わせ  
10 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 42 publication(s) in Google Scholar

Other Forms of OSMI-1:

OSMI-1 関連抗体

Top Publications Citing Use of Products

顧客検証

WB
ELISA
RT-PCR
Histological Imaging/Staining

    OSMI-1 purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2025 Jan;22(1):68-82.  [Abstract]

    Representative western blot showing changes in O-GlcNAc levels in total bone marrow (BM) cells and early B (CD11b–IgM–B220+) cells from mice intraperitoneally injected with OSMI-1 (1 mg/day 10 days).

    OSMI-1 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Jan 4;15(1):252.  [Abstract]

    ELISA was performed to determine IFN-β concentration in the BALF of the indicated mice (Intraperitoneally with 200 µl 1 mg/kg OSMI-1 6 h before PR8 infection, followed by once-daily administration on days 14–15).

    OSMI-1 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Jan 4;15(1):252.  [Abstract]

    Relative influenza virus content in the mice (Intraperitoneally with 200 µl 1 mg/kg OSMI-1 6 h before PR8 infection, followed by once-daily administration on days 14–15) lung tissues was quantified based on M protein levels measured via RT-qPCR and shown as ΔCt fold change.

    OSMI-1 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Jan 4;15(1):252.  [Abstract]

    Periodic acid-Schiff (PAS), hematoxylin and eosin (H&E), and Masson’s trichome-stained lung tissues of the indicated mice (Intraperitoneally with 200 µl 1 mg/kg OSMI-1 6 h before PR8 infection, followed by once-daily administration on days 14–15).

    OSMI-1 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Jan 4;15(1):252.  [Abstract]

    Immunoblotting of P-TBK1, TBK1, P-IRF3, IRF3, and β-actin in lysates of BMDMs stimulated by 4 μg/ml Poly(I:C) for the indicated times and pretreated with 20 μg/ml OSMI-1 for 30 min.

    OSMI-1 purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2023 Feb 10;8(1):63.  [Abstract]

    OSMI-1 (20 μΜ; 12 h) downregulates the O-GlcNAcylation in both HepG2-HBV1.3 cells (fig j) and Huh7-HBV1.3 cells (fig k).

    Acyltransferase アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    ACAT1 ACAT2 MGAT2 ACAT DGAT1 DGAT2

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    OSMI-1 is a cell-permeable O-GlcNAc transferase (OGT) inhibitor with an IC50 value of 2.7 μM. OSMI-1 inhibits protein O-linked N-acetylglucosamine (O-GlcNAcylation) in several mammalian cell lines without qualitatively altering cell surface N- or O-linked glycans[1][2].

    IC50 & Target

    IC50: 2.7 μM (O-GlcNAc transferase)[1]
    体外実験

    OSMI-1 (50 μM; 24 hours; CHO cells) treatment decreases the viability by about 50% after 24 hours[1].
    OSMI-1 (10-100 μM; 24 hours; CHO cells) treatment reduces global O-linked N-acetylglucosamine (O-GlcNAcylation) a dose-dependent manner. OSMI-1 inhibits OGT activity in cells[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    OSMI-1 関連抗体

    Cell Viability Assay[1]

    Cell Line: CHO cells
    Concentration: 50 μM
    Incubation Time: 24 hours
    Result: Viability decreased by about 50% after 24 hours.

    Western Blot Analysis[1]

    Cell Line: CHO cells
    Concentration: 10 μM, 25 μM, 50 μM, 100 μM
    Incubation Time: 24 hours
    Result: Reduced global OGlcNAcylation in a dose-dependent manner.
    体内実験

    Mammalian and zebrafish toxicity profiles are strikingly similar, and zebrafish can usually serve as an intermediate step between cell-based evaluation and conventional animal testing. The zebrafish model is used to investigate OSM1-1 acute toxicity in vivo. The LC50 of OSM1-1 is 0.031 mg/mL (56 μM, 12 h) and 0.025 mg/mL (45 μM, 24 h) in zebrafish model[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    563.64

    分子式

    C28H25N3O6S2
    CAS 番号
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    O=C(N(CC1=CC=CS1)CC2=CC=CO2)[C@H](NS(C3=CC(C=C4)=C(C=C3)NC4=O)(=O)=O)C5=CC=CC=C5OC
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 100 mg/mL (177.42 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.7742 mL 8.8709 mL 17.7418 mL
    5 mM 0.3548 mL 1.7742 mL 3.5484 mL
    10 mM 0.1774 mL 0.8871 mL 1.7742 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: 2.08 mg/mL (3.69 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.08 mg/mL (3.69 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.88%

    COA (257 KB) HNMR (107 KB) RP-HPLC (224 KB) MS (68 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.7742 mL 8.8709 mL 17.7418 mL 44.3546 mL
    5 mM 0.3548 mL 1.7742 mL 3.5484 mL 8.8709 mL
    10 mM 0.1774 mL 0.8871 mL 1.7742 mL 4.4355 mL
    15 mM 0.1183 mL 0.5914 mL 1.1828 mL 2.9570 mL
    20 mM 0.0887 mL 0.4435 mL 0.8871 mL 2.2177 mL
    25 mM 0.0710 mL 0.3548 mL 0.7097 mL 1.7742 mL
    30 mM 0.0591 mL 0.2957 mL 0.5914 mL 1.4785 mL
    40 mM 0.0444 mL 0.2218 mL 0.4435 mL 1.1089 mL
    50 mM 0.0355 mL 0.1774 mL 0.3548 mL 0.8871 mL
    60 mM 0.0296 mL 0.1478 mL 0.2957 mL 0.7392 mL
    80 mM 0.0222 mL 0.1109 mL 0.2218 mL 0.5544 mL
    100 mM 0.0177 mL 0.0887 mL 0.1774 mL 0.4435 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    OSMI-1 Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    OSMI-11681056-61-0OSMI1OSMI 1OGTAcyltransferaseO-linked N-acetylglucosaminyltransferaseO-GlcNAc transferaseDiacylglycerol acyltransferaseDiglyceride acyltransferaseacyl-CoA:cholesterol acyltransferasemono- acylglycerol acyltransferaseO-GlcNActransferasemammaliancell-permeableO-GlcNAcylationtoxicityzebrafishInhibitorinhibitorinhibit

    最近チェックした製品:

    オンラインお問い合わせ

    Your information is safe with us. * Required Fields.

    製品名

    		  

    カスタマ需要量 *

    お名前 *

    		 

    タイトル

    メールアドレス *

    		 

    電話番号 *

    デパートメント

    		 

    組纖名 *

    市区町村

    都道府県

    国或いは地域 *

    		      

    必ず会社名を記載ください。個人への返信は行いません。

    備考

    バルクお問い合わせ

    Inquiry Information

    製品名:
    OSMI-1
    製品番号:
    HY-119738
    数量:
    MCE 日本正規代理店:

    カスタマーレビュー

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    製品名

    		  

    Lot #

    お名前 *

    		 

    メールアドレス *

    電話番号 *

    		 

    部門 *

    組纖名 *

    		 

    国或いは地域 *

    備考

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.