2. Vitamin D Related/Nuclear Receptor
  3. Estrogen Receptor/ERR
  4. Propyl pyrazole triol

Propyl pyrazole triol  (Synonyms: PPT)

製品番号: HY-100689 純度: 99.11%
COA 取扱説明書 Technical Support

Propyl pyrazole triol (PPT) is a selective estrogen receptor alpha (ERα) agonist. The relative binding affinity of Propyl pyrazole triol for ERα (ERα: 49%) around 410 times higher compared with estrogen receptor beta (ERβ: 0.12%).

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CAS 番号 : 263717-53-9

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 121 在庫あり
Solution
10 mM * 1 mL in DMSO USD 121 在庫あり
Solid
5 mg $110 在庫あり
10 mg $176 在庫あり
25 mg $340 在庫あり
50 mg $510 在庫あり
100 mg $765 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 18 publication(s) in Google Scholar

Other Forms of Propyl pyrazole triol:

Propyl pyrazole triol 関連抗体

Top Publications Citing Use of Products

    Propyl pyrazole triol purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2022 Feb 21;18(5):1961-1973.

    Propyl pyrazole triol (PPT) (5 nM) promoted the expansion of CD4+CD25+ peritoneal regulatory T cells (Tregs).

    Propyl pyrazole triol purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2022 Feb 21;18(5):1961-1973.

    Propyl pyrazole triol (PPT) (5 nM) notably increased the production of IL-10 and TGF-β1 in CD4+CD25+ peritoneal regulatory T cells (Tregs).

    Propyl pyrazole triol purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2021 Feb 1;498:54-69.  [Abstract]

    ERα-specific agonist Propyl pyrazole triol (PPT) (1 nM; 48 h) enhanced luciferase activity in MCF-7 cells.

    Propyl pyrazole triol purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2021 Feb 1;498:54-69.  [Abstract]

    ERα-specific agonist Propyl pyrazole triol (PPT) (1 nM; 48 h) induced proliferation of MCF-7 cells.

    Propyl pyrazole triol purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2021 Feb 1;498:54-69.  [Abstract]

    ERα-specific agonist Propyl pyrazole triol (PPT) (1 nM; 48 h) induced migration and invasion of MCF-7 cells.

    Estrogen Receptor/ERR アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Estrogen receptor ERα ERβ ERRα ERRβ ERRγ

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Propyl pyrazole triol (PPT) is a selective estrogen receptor alpha (ERα) agonist. The relative binding affinity of Propyl pyrazole triol for ERα (ERα: 49%) around 410 times higher compared with estrogen receptor beta (ERβ: 0.12%)[1][2].

    IC50 & Target[1]

    ERα

     

    Cellular Effect
    Cell Line Type Value Description References
    MCF7 IC50
    27.2 μM
    Compound: PPT
    Cytotoxicity in hormone-dependent human MCF7 cells assessed as growth inhibition incubated for 4 day s by crystal violet assay
    Cytotoxicity in hormone-dependent human MCF7 cells assessed as growth inhibition incubated for 4 day s by crystal violet assay
    		[PMID: 23043242]
    U2OS EC50
    0.1 nM
    Compound: PPT
    Activation of human ERalpha expressed in human U2-OS cells by luciferase reporter gene assay relative to untreated control
    Activation of human ERalpha expressed in human U2-OS cells by luciferase reporter gene assay relative to untreated control
    		[PMID: 23043242]
    体外実験

    The EC50 of Propyl pyrazole triol for the ERα-dependent response is 140 pM in ERα-U2OS-Luc cells[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Propyl pyrazole triol 関連抗体
    分子量

    386.44

    分子式

    C24H22N2O3
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    CCCC1=C(C2=CC=C(O)C=C2)N(C3=CC=C(O)C=C3)N=C1C4=CC=C(O)C=C4
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : ≥ 100 mg/mL (258.77 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.5877 mL 12.9386 mL 25.8772 mL
    5 mM 0.5175 mL 2.5877 mL 5.1754 mL
    10 mM 0.2588 mL 1.2939 mL 2.5877 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 5 mg/mL (12.94 mM); Clear solution

      This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (6.47 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.11%

    COA (253 KB) RP-HPLC (332 KB) Elemental Analysis Report (111 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.5877 mL 12.9386 mL 25.8772 mL 64.6931 mL
    5 mM 0.5175 mL 2.5877 mL 5.1754 mL 12.9386 mL
    10 mM 0.2588 mL 1.2939 mL 2.5877 mL 6.4693 mL
    15 mM 0.1725 mL 0.8626 mL 1.7251 mL 4.3129 mL
    20 mM 0.1294 mL 0.6469 mL 1.2939 mL 3.2347 mL
    25 mM 0.1035 mL 0.5175 mL 1.0351 mL 2.5877 mL
    30 mM 0.0863 mL 0.4313 mL 0.8626 mL 2.1564 mL
    40 mM 0.0647 mL 0.3235 mL 0.6469 mL 1.6173 mL
    50 mM 0.0518 mL 0.2588 mL 0.5175 mL 1.2939 mL
    60 mM 0.0431 mL 0.2156 mL 0.4313 mL 1.0782 mL
    80 mM 0.0323 mL 0.1617 mL 0.3235 mL 0.8087 mL
    100 mM 0.0259 mL 0.1294 mL 0.2588 mL 0.6469 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Propyl pyrazole triol Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Propyl pyrazole triol263717-53-9PPTEstrogen Receptor/ERRInhibitorinhibitorinhibit

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    製品名:
    Propyl pyrazole triol
    製品番号:
    HY-100689
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