1. Anti-infection
  2. Bacterial

Rifampicin (Synonyms: Rifampin; Rifamycin AMP)

Cat. No.: HY-B0272 Purity: 96.61%
Handling Instructions

Rifampicin is a broad-spectrum antibiotic, which inhibits the bacterial DNA-dependent RNA polymerase.

For research use only. We do not sell to patients.

Rifampicin Chemical Structure

Rifampicin Chemical Structure

CAS No. : 13292-46-1

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 75 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
1 g USD 68 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
5 g USD 270 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Rifampicin is a broad-spectrum antibiotic, which inhibits the bacterial DNA-dependent RNA polymerase.

In Vitro

Rifampicin (100 mg/mL) can block the functional activity of P-glycoprotein. Rifampicin is not a substract for P-glycoprotein. The mechanism of rifampicin resistance is unassociated with the functional activity of P-glycoprotein[3].

In Vivo

Rifampicin (200, 400 mg/kg) can induce fatty liver at high concentration[1]. Rifampicin (30 mg/kg, i.p.) treatment of S464P biofilms in vivo results in a slight decline, but earlier rebinds in bioluminescence from these catheters compared with the parental signal, whereas rifampicin has no affect on bioluminescence in mice infected with mutant H481Y[2].

Clinical Trial
References
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 1.2152 mL 6.0758 mL 12.1516 mL
5 mM 0.2430 mL 1.2152 mL 2.4303 mL
10 mM 0.1215 mL 0.6076 mL 1.2152 mL
Please refer to the solubility information to select the appropriate solvent.
Animal Administration
[2]

Rifampicin is formulated in saline.

Briefly, 1 cm Teflon catheter (14-gauge) carrying 104 cfu S. aureus, either the parental strain Xen 29 or the RifR mutants S464P or H481Y, are implanted subcutaneously in groups of nine mice per strain. One catheter segment is inserted on each side of each animal. Six days after the implantation of the catheters, five mice from each group are treated with rifampicin at 30 mg/kg intraperitoneally in 0.1 mL saline, twice daily for four consecutive days. The remaining four mice in each group are left untreated as controls. At various time points during the infection, the mice are anaesthetized using a constant flow of 1.5% isoflurane from the IVIS® manifold, and imaged using an IVIS® Image System 100 Series. The bioluminescent signals (photons/s) emitted from the mice are analysed using LivingImage® software and plotted over the course of infection. The mice are sacrificed 20 days after infection (11 days after final rifampicin treatment). The catheters are surgically removed and the bacteria are detached by sonication for determination of bacterial burdens on the catheters. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

822.94

Formula

C₄₃H₅₈N₄O₁₂

CAS No.

13292-46-1

SMILES

OC(C(/C=N/N1CCN(C)CC1)=C(NC(/C(C)=C\C=C\[[email protected]@H]([[email protected]@H]([[email protected]@H](C)[[email protected]]2O)O)C)=O)C(O)=C3C(O)=C4C)=C3C5=C4O[[email protected]](C)(O/C=C/[[email protected]@H]([[email protected]]([[email protected]@]([[email protected]@H]2C)([H])OC(C)=O)C)OC)C5=O

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

10 mM in DMSO

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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Rifampicin
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HY-B0272
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