2. Immunology/Inflammation
  3. NOD-like Receptor (NLR)
  4. Ruscogenin

ルスコゲニン  (Synonyms: Ruscogenin)

製品番号: HY-N0496 純度: 99.85%
COA 取扱説明書 Technical Support

Ruscogenin, an important steroid sapogenin derived from Ophiopogon japonicus, attenuates cerebral ischemia-induced blood-brain barrier dysfunction by suppressing TXNIP/NLRP3 inflammasome activation and the MAPK pathway. Ruscogenin exerts significant anti-inflammatory and anti-thrombotic activities. Ruscogenin has orally bioactivity.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 472-11-7

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 108 在庫あり
Solution
10 mM * 1 mL in DMSO USD 108 在庫あり
Solid
1 mg $47 在庫あり
5 mg $99 在庫あり
10 mg $158 在庫あり
25 mg $300 在庫あり
50 mg $450 在庫あり
100 mg $675 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 6 publication(s) in Google Scholar

Other Forms of Ruscogenin:

ルスコゲニン 関連抗体

Top Publications Citing Use of Products

    Ruscogenin purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 Oct 7;143(Pt 1):113336.  [Abstract]

    The CD86, iNOS, and CD206 protein levels in RAW264.7 following LPS and Ruscogenin (RUS) (5, 25 μM) treatment were quantified by western blotting.

    Ruscogenin purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 Oct 7;143(Pt 1):113336.  [Abstract]

    Illustrative images of immunofluorescence staining: red ( iNOS/CD206), green (cytoskeleton), and blue (nuclei) following LPS and Ruscogenin (RUS) (5, 25 μM) treatment.

    Ruscogenin purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 Oct 7;143(Pt 1):113336.  [Abstract]

    Flow cytometry was used to examine CD86+ and CD206+ cells following LPS and Ruscogenin (RUS) (5, 25 μM) treatment.

    Ruscogenin purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 Oct 7;143(Pt 1):113336.  [Abstract]

    S F and toluidine blue staining of chondrocytes following LPS and Ruscogenin (RUS) (5, 25 μM) treatment.

    Ruscogenin purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 Oct 7;143(Pt 1):113336.  [Abstract]

    Illustrative images of S.O and HE staining of ACLT or Ruscogenin (RUS) (5, 10 mg/kg) rat samples at 8 weeks post-surgery.

    NOD-like Receptor (NLR) アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    NLRP3 NLRP1 NOD1 NOD2

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Ruscogenin, an important steroid sapogenin derived from Ophiopogon japonicus, attenuates cerebral ischemia-induced blood-brain barrier dysfunction by suppressing TXNIP/NLRP3 inflammasome activation and the MAPK pathway. Ruscogenin exerts significant anti-inflammatory and anti-thrombotic activities. Ruscogenin has orally bioactivity[1][2].

    IC50 & Target

    NLRP3

     

    Cellular Effect
    Cell Line Type Value Description References
    BXPC-3 IC50
    7.32 μM
    Compound: 48
    Anticancer activity against human BXPC-3 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
    Anticancer activity against human BXPC-3 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
    		[PMID: 36332549]
    SW1990 IC50
    8.14 μM
    Compound: 48
    Anticancer activity against human SW1990 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
    Anticancer activity against human SW1990 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
    		[PMID: 36332549]
    THP-1 IC50
    2.27 μM
    Compound: 1
    Anticoagulant activity in human THP1 cells assessed as inhibition of TNF-alpha-stimulated tissue factor expression incubated 1 hr before TNFalpha challenge measured after 5 hrs
    Anticoagulant activity in human THP1 cells assessed as inhibition of TNF-alpha-stimulated tissue factor expression incubated 1 hr before TNFalpha challenge measured after 5 hrs
    		[PMID: 20561785]
    体外実験

    Ruscogenin (0.01-40 μM; 24 h) increases the cell viability in bEnd.3 cells subjected to OGD/R[1].
    Ruscogenin (0.01-10 μM; 24 h) reverts the endothelial barrier leakage, inhibits the expression of IL-Iβ and Caspase-1, and modulats the TXNIP/NLRP3 pathway in bEnd.3 cells subjected to OGD/R[1].
    Ruscogenin (0.01-10 μM; 4 h) inhibits the production of ROS, and regulated the MAPK Pathway in bEnd.3 Cells subjected to OGD/R[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    ルスコゲニン 関連抗体

    Cell Viability Assay[1]

    Cell Line: bEnd.3 cell
    Concentration: 0.01 μM, 0.1 μM, 1 μM, 10 μM, 20 μM, 40 μM
    Incubation Time: 24 h
    Result: Showed that the cell viability reduction in OGD/R-induced bEnd.3 cell was significantly recovered.

    Immunofluorescence[1]

    Cell Line: bEnd.3 cell
    Concentration: 0.1 μM, 1 μM, 10 μM
    Incubation Time: 24 h
    Result: Demonstrated increase in the TEER value and inhibition the sodium fluorescein permeability.

    Western Blot Analysis[1]

    Cell Line: bEnd.3 cell
    Concentration: 0.01 μM, 0.1 μM, 1 μM, 10 μM, 20 μM, 40 μM
    Incubation Time: 24 h
    Result: Demonstrated downregulation of the expression of IL-1β and Caspase-1 proteins, and inhibition in the expressions of NLRP3 and TXNIP.

    Immunofluorescence[1]

    Cell Line: bEnd.3 cell
    Concentration: 0.01 μM, 0.1 μM, 1 μM, 10 μM, 20 μM, 40 μM
    Incubation Time: 4 h
    Result: Demonstrated reduction in the production of ROS.
    体内実験

    Ruscogenin (10 mg/kg; i.g.; 1 time) improves the MCAO/R-induced brain tissue injury and inhibits the expression of IL-1β and Caspase-1 and modulats the TXNIP/NLRP3[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: MCAO/R Mice[1]
    Dosage: 10mg/kg
    Administration: Oral Gavage (i.g.)
    Result: Showed smaller infarct size, ameliorating histopathological damage by decreasing the cell loss, and significant increase in CBF (cerebral blood flow) compared to the model group.
    Resulted inhibiting the expression of IL-1β and Caspase-1, NLRP3 and TXNIP compared to the model group.
    分子量

    430.62

    分子式

    C27H42O4
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    C[C@@]12[C@]([C@@H]3C)([H])[C@](O[C@]34CC[C@@H](C)CO4)([H])C[C@@]1([H])[C@@](CC=C(C[C@@H](O)C5)[C@@]6([C@@H]5O)C)([H])[C@]6([H])CC2
    Structure Classification
    Initial Source
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
    溶剤 & 溶解度
    体外: 

    Ethanol : 25 mg/mL (58.06 mM; Need ultrasonic)

    DMSO : 4.31 mg/mL (10.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.3222 mL 11.6112 mL 23.2223 mL
    5 mM 0.4644 mL 2.3222 mL 4.6445 mL
    10 mM 0.2322 mL 1.1611 mL 2.3222 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 1 mg/mL (2.32 mM); Clear solution

      This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  50% PEG300    50% Saline

      Solubility: 1.67 mg/mL (3.88 mM); Suspended solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.85%

    COA (250 KB) HNMR (271 KB) RP-HPLC (240 KB) MS (76 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / Ethanol 1 mM 2.3222 mL 11.6112 mL 23.2223 mL 58.0558 mL
    5 mM 0.4644 mL 2.3222 mL 4.6445 mL 11.6112 mL
    10 mM 0.2322 mL 1.1611 mL 2.3222 mL 5.8056 mL
    Ethanol 15 mM 0.1548 mL 0.7741 mL 1.5482 mL 3.8704 mL
    20 mM 0.1161 mL 0.5806 mL 1.1611 mL 2.9028 mL
    25 mM 0.0929 mL 0.4644 mL 0.9289 mL 2.3222 mL
    30 mM 0.0774 mL 0.3870 mL 0.7741 mL 1.9352 mL
    40 mM 0.0581 mL 0.2903 mL 0.5806 mL 1.4514 mL
    50 mM 0.0464 mL 0.2322 mL 0.4644 mL 1.1611 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Ruscogenin Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Ruscogenin472-11-7NOD-like Receptor (NLR)Cardiovascular DiseaseAnti-inflammationInhibitorinhibitorinhibit

    最近チェックした製品:

    オンラインお問い合わせ

    Your information is safe with us. * Required Fields.

    製品名

    		  

    カスタマ需要量 *

    お名前 *

    		 

    タイトル

    メールアドレス *

    		 

    電話番号 *

    デパートメント

    		 

    組纖名 *

    市区町村

    都道府県

    国或いは地域 *

    		      

    必ず会社名を記載ください。個人への返信は行いません。

    備考

    バルクお問い合わせ

    Inquiry Information

    製品名:
    Ruscogenin
    製品番号:
    HY-N0496
    数量:
    MCE 日本正規代理店:

    カスタマーレビュー

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    製品名

    		  

    Lot #

    お名前 *

    		 

    メールアドレス *

    電話番号 *

    		 

    部門 *

    組纖名 *

    		 

    国或いは地域 *

    備考

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.