46 Results for "

β-galactosidase substrate

" in MedChemExpress (MCE) Product Catalog:
Products (46)

46 Results for "β-galactosidase substrate" in MCE Product Catalog:

12
12 Publications Verification
Cat. No.: HY-126839
CAS No.: 138777-25-0
Purity:  99.82%
Synonyms: 5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside
C12FDG (5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside) is a lipophilic green fluorescent substrate for β-galactosidase detection. C12-FDG is more sensitive than Fluorescein di(β-D-galactopyranoside) (HY-101895) for beta-galactosidase activity determinations in animal cells (Ex/Em = 488/523 nm) .
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7
7 Cited Publications
Cat. No.: HY-101895
CAS No.: 17817-20-8
Purity:  99.81%
Synonyms: FDG
Fluorescein di(β-D-galactopyranoside) is a fluorogenic substrate for β-galactosidaseex=485 nm, λem=535 nm).
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7
7 Cited Publications
Cat. No.: HY-15926
CAS No.: 369-07-3
Synonyms: 2-Nitrophenyl β-D-galactopyranoside
ONPG is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activity.
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6
6 Cited Publications
Cat. No.: HY-15934
CAS No.: 7240-90-6
Synonyms: BCIG
X-GAL (BCIG) is a widely used chromogenic β-galactosidase substrate. X-GAL is a colorless compound until cleaved by β-galactosidase, at which point X-GAL turns to an insoluble and detectable blue compound, making X-GAL particularly useful in techniques such as blue-white screening for cloning in bacteria. X-GAL can also be used for detection of β-galactosidase activity .
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2
2 Cited Publications
Cat. No.: HY-D1224
CAS No.: 99792-79-7
Synonyms: CPRG
Chlorophenol red-β-D-galactopyranoside (CPRG) is an efficient and sensitive chromogenic substrate for β-galactosidase (HY-P2869), widely used in colorimetric assays. Chlorophenol red-β-D-galactopyranoside itself appears pale yellow. When it is specifically hydrolyzed by β-galactosidase, it releases chlorophenol red. The released chlorophenol red turns purple-red under alkaline or neutral pH conditions. This color change from yellow to red can be quantitatively detected at wavelengths of 540-572 nm using visible spectrophotometry .
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1
1 Cited Publications
Cat. No.: HY-P2803A
CAS No.: 9001-45-0
Target:  

β-glucuronidase

Research Areas:  

Metabolic Disease

Beta-glucuronidase (bovine liver) is a glycoside hydrolase that hydrolyzes β-glucuronic acid and sulfate esters in urine and other biological fluids, thereby releasing β-glucuronides. Beta-glucuronidase (bovine liver) cannot effectively hydrolyze Estriol glucuronide. Beta-glucuronidase (bovine liver) can be used in studies related to systemic gangliosidosis .
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1
1 Cited Publications
Cat. No.: HY-137845
CAS No.: 6160-78-7
Purity:  99.06%
4-Methylumbelliferyl β-D-galactopyranoside is a fluorescent substrate for β-galactosidase which, when cleaved, produces a water-soluble blue fluorescent coumarin fluorophore that can be detected using a fluoroenzymeter or fluorometer .
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1
1 Cited Publications
Cat. No.: HY-D1633A
CAS No.: 206443-06-3
4-Methylumbelliferyl-β-D-galactopyranoside 6-sulfate sodium is a fluorescent dye. 4-Methylumbelliferyl-β-D-galactopyranoside 6-sulfate sodium undergoes desulphation by galactose-6-sulphate sulphatase to form 4-methylumbelliferyl-β-D-galactopyranoside, which is cleaved by β-galactosidase to release fluorescent 4-methylumbelliferone. 4-Methylumbelliferyl-β-D-galactopyranoside 6-sulfate sodium interacts with N-acetylgalactosamine-6-sulfate sulfatase (GALNS) via hydrogen bonds, electrostatic interactions, and steric interactions. 4-Methylumbelliferyl-β-D-galactopyranoside 6-sulfate sodium serves as a substrate in assays measuring galactose-6-sulphate sulphatase and GALNS activity. 4-Methylumbelliferyl-β-D-galactopyranoside 6-sulfate sodium can be used for the research of Morquio disease type A (mucopolysaccharidosis IV A) .
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Cat. No.: HY-137276
CAS No.: 97753-82-7
Purity:  99.73%
Synonyms: Bluo-Gal
Research Areas:  

Others

5-Bromo-3-indolyl β-D-galactopyranoside (Bluo-Gal) is a chromogenic substrate for β-galactosidase. 5-Bromo-3-indolyl β-D-galactopyranoside is hydrolyzed by the enzyme to generate a 5-bromoindole intermediate, which is further oxidized to form an insoluble blue precipitate. 5-Bromo-3-indolyl β-D-galactopyranoside can specifically recognize bacterial β-galactosidases (such as the product of the Escherichia coli lacZ gene) and reacts at pH 7.4, making it suitable for light and electron microscopic observations. 5-Bromo-3-indolyl β-D-galactopyranoside can be used in histochemical detection of reporter gene expression in transgenic organisms, such as the localization analysis of β-galactosidase activity in mouse embryos or muscle tissues .
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Cat. No.: HY-DY2002
CAS No.: 7240-90-6
X-GAL (BCIG) (solution) is a widely used chromogenic β-galactosidase substrate. X-GAL is a colorless compound until cleaved by β-galactosidase, at which point X-GAL turns to an insoluble and detectable blue compound, making X-GAL particularly useful in techniques such as blue-white screening for cloning in bacteria. X-GAL can also be used for detection of β-galactosidase activity .
Solvent and Concentration: DMF: 20 mg/mL
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Cat. No.: HY-P3185
CAS No.: 9028-54-0
Research Areas:  

Metabolic Disease

Beta-galactose dehydrogenase is a selective catalyst for β-galactose. Under pH 8.6 conditions, beta-galactose dehydrogenase catalyzes the oxidation of β-galactose, produced by the hydrolysis of lactose by β-galactosidase, with nicotinamide adenine dinucleotide (NAD) to produce reduced nicotinamide adenine dinucleotide (NADH). Beta-galactose dehydrogenase specifically mediates this oxidation reaction for the quantitative detection of the substrate, used in the analysis of lactose concentration in samples such as breast milk .
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Cat. No.: HY-W011654
CAS No.: 5094-33-7
Synonyms: 4-Aminophenyl-beta-D-galactopyranoside, 98%
4-Aminophenyl-β-D-galactopyranoside, 98% is a highly efficient substrate for β-galactosidase. It is specifically hydrolyzed by this enzyme to release galactose and electroactive p-aminophenol. 4-Aminophenyl-β-D-galactopyranoside, 98% is widely used in colorimetric and electrochemical assays for detecting β-galactosidase activity and determining enzyme kinetics, such as in biosensing fields including cellular senescence, pathogen and contaminant detection. In addition, since β-galactosidase is often overexpressed in primary ovarian cancer, 4-Aminophenyl-β-D-galactopyranoside, 98% can also be applied to related research on primary ovarian cancer .
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Cat. No.: HY-DY1033
CAS No.: 138777-25-0
C12FDG (5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside) (solution) is a lipophilic green fluorescent substrate for β-galactosidase detection. C12-FDG is more sensitive than Fluorescein di (β-D-galactopyranoside) (HY-101895) for beta-galactosidase activity determinations in animal cells (Ex/Em = 488/523 nm) .
Solvent and concentration: DMSO: 10 mM
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Cat. No.: HY-W127769
CAS No.: 138182-21-5
Synonyms: Salmon-Gal
Rose-β-D-Gal is a chromogenic substrate, is also a β-galactosidase substrate. Rose-β-D-Gal creates a pink/magenta color after the reaction and has been used for detection of β-gal activity .
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Cat. No.: HY-137249
CAS No.: 95079-19-9
Purity:  98.32%
Resorufin-β-D-galactopyranoside is a commonly used substrate in various biochemical assays to measure the activity of β-galactosidase, an important enzyme involved in lactose metabolism and regulation of gene expression. Resorufin-β-D-galactopyranoside has unique chemical properties and can be hydrolyzed by β-galactosidase to form a red fluorescent product called resorufin. This makes it a useful tool for detecting and quantifying β-galactosidase activity in biological samples such as bacteria or mammalian cells.
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Cat. No.: HY-137779
CAS No.: 15548-60-4
Purity:  99.11%
5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside, a chromogenic substrate for the detection of β-galactosidase activity. It is commonly used in molecular biology techniques such as gene expression analysis and reporter gene analysis. When β-galactosidase cleaves X-Gluc, a blue precipitate is produced, which can be observed by microscopy or other detection methods. X-Gluc has high sensitivity and specificity for the detection of β-galactosidase activity, making it a widely used tool in molecular biology research.
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Cat. No.: HY-D1461
CAS No.: 129787-66-2
Fluorescein Di-β-D-Glucopyranoside is a specific β-glucocerebrosidase substrate that can be used for the intralysosomal β-galactosidase .
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Cat. No.: HY-15926S
CAS No.: 864933-71-1
Synonyms: 2-Nitrophenyl β-D-galactopyranoside-13C
ONPG- 13C is the 13C labeled ONPG. ONPG is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activit .
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Cat. No.: HY-D1699
CAS No.: 209540-64-7
PFB-FDG is a non-fluorescent galactosidase substrate that can be hydrolysed to green fluorescent PFB-F (Ex=485 nm, Em=535 nm). PFB-FDG can be used for the determination of β-galactosidase activity .
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Cat. No.: HY-114958
CAS No.: 107610-19-5
RH 421 is a voltage-sensitive styryl dye that can penetrate through the lipid bilayers. RH 421 is a chromogenic substrate for β-galactosidase .
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