PFB-FDG 

PFB-FDG is a non-fluorescent galactosidase substrate that can be hydrolysed to green fluorescent PFB-F (Ex=485 nm, Em=535 nm). PFB-FDG can be used for the determination of β-galactosidase activity^[1].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Measurement of β-galactosidase activity^[1]:
1. Incubate 2 × 10⁵ cells with 50 μg/mL of PFB-FDG in serum-free DMEM containing 25 mM Hepes (pH 7.4) for 2 h at 37℃. (Mononuclear cells for example, different cell concentrations vary)
2. Wash cells once with ice-cold PBS.
3. Analyze cells by flow cytometry (Ex=485 nm, Em=535 nm).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

865.66

C₃₉H₃₂F₅NO₁₆

209540-64-7

535

488

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Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Navarrete Santos A, et al. Evidences for age-related modulation of human hematopoietic progenitor cell proliferation. Exp Gerontol. 2008 Nov;43(11):1033-8.  [Content Brief]