PFB-FDG
Based on 1 Customer Validation
PFB-FDG is a non-fluorescent galactosidase substrate that can be hydrolysed to green fluorescent PFB-F (Ex=485 nm, Em=535 nm). PFB-FDG can be used for the determination of β-galactosidase activity.
For research use only. We do not sell to patients.
- Purity: 98.19%
- CAS No.: 209540-64-7
- Formula: C39H32F5NO16
- Molecular Weight:865.66
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Measurement of β-galactosidase activity[1]:
1. Incubate 2 × 105 cells with 50 μg/mL of PFB-FDG in serum-free DMEM containing 25 mM Hepes (pH 7.4) for 2 h at 37°C. (Mononuclear cells for example, different cell concentrations vary)
2. Wash cells once with ice-cold PBS.
3. Analyze cells by flow cytometry (Ex = 485 nm, Em = 535 nm).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
535
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Chemical Information
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CAS No. 209540-64-7
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Appearance Solid
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Molecular Weight 865.66
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Formula C39H32F5NO16
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Color Light yellow to yellow
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SMILES
O[C@H]([C@H]1O)[C@@H](O)[C@@H](CO)O[C@H]1OC(C=C2)=CC3=C2C4(OC(C5=C4C=CC(NC(C6=C(F)C(F)=C(F)C(F)=C6F)=O)=C5)=O)C7=C(O3)C=C(O[C@@H]8O[C@H](CO)[C@H](O)[C@H](O)[C@H]8O)C=C7
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (115.52 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (278 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.1552 mL | 5.7759 mL | 11.5519 mL | 28.8797 mL |
| 5 mM | 0.2310 mL | 1.1552 mL | 2.3104 mL | 5.7759 mL | |
| 10 mM | 0.1155 mL | 0.5776 mL | 1.1552 mL | 2.8880 mL | |
| 15 mM | 0.0770 mL | 0.3851 mL | 0.7701 mL | 1.9253 mL | |
| 20 mM | 0.0578 mL | 0.2888 mL | 0.5776 mL | 1.4440 mL | |
| 25 mM | 0.0462 mL | 0.2310 mL | 0.4621 mL | 1.1552 mL | |
| 30 mM | 0.0385 mL | 0.1925 mL | 0.3851 mL | 0.9627 mL | |
| 40 mM | 0.0289 mL | 0.1444 mL | 0.2888 mL | 0.7220 mL | |
| 50 mM | 0.0231 mL | 0.1155 mL | 0.2310 mL | 0.5776 mL | |
| 60 mM | 0.0193 mL | 0.0963 mL | 0.1925 mL | 0.4813 mL | |
| 80 mM | 0.0144 mL | 0.0722 mL | 0.1444 mL | 0.3610 mL | |
| 100 mM | 0.0116 mL | 0.0578 mL | 0.1155 mL | 0.2888 mL |
PFB-FDG Related Classifications
HY-D1699 Related Classifications
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