2. Others Metabolic Enzyme/Protease
  3. Fluorescent Dye Glycosidase
  4. HMRef-βGal

HMRef-βGal is a fluorescent probe and a substrate responsive to β-galactosidase (β-galactosidase) (Ex/Em=498 nm/505-600 nm). After being cleaved by β-galactosidase, HMRef-βGal triggers significant fluorescence enhancement via intramolecular spirocyclic function regulation. HMRef-βGal generates bright fluorescence in cancer cells with elevated β-galactosidase activity, enabling visualization of tiny peritoneal metastases in mouse models. HMRef-βGal exhibits low in vitro cytotoxicity and low acute in vivo toxicity in mice. HMRef-βGal can be used for preclinical fluorescence-guided diagnosis and cytoreductive surgery of peritoneal metastases, including compatibility with real-time naked-eye detection and endoscopic imaging, as well as for studies related to peritoneal metastases of ovarian cancer.

For research use only. We do not sell to patients.

HMRef-βGal

HMRef-βGal Chemical Structure

CAS No. : 2163840-29-5

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HMRef-βGal Related Antibodies

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Description

HMRef-βGal is a fluorescent probe and a substrate responsive to β-galactosidase (β-galactosidase) (Ex/Em=498 nm/505-600 nm). After being cleaved by β-galactosidase, HMRef-βGal triggers significant fluorescence enhancement via intramolecular spirocyclic function regulation. HMRef-βGal generates bright fluorescence in cancer cells with elevated β-galactosidase activity, enabling visualization of tiny peritoneal metastases in mouse models. HMRef-βGal exhibits low in vitro cytotoxicity and low acute in vivo toxicity in mice. HMRef-βGal can be used for preclinical fluorescence-guided diagnosis and cytoreductive surgery of peritoneal metastases, including compatibility with real-time naked-eye detection and endoscopic imaging, as well as for studies related to peritoneal metastases of ovarian cancer[1].

In Vitro

HMRef-βGal (10 μM; 1 h) sensitively detects intracellular β-galactosidase activity in SHIN3, SKOV3, OVK18, OVCAR3, OVCAR4, OVCAR5, and OVCAR8 human ovarian cancer cell lines via live-cell confocal imaging (Ex/Em=498 nm/505-600 nm)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

HMRef-βGal Related Antibodies
In Vivo

HMRef-βGal (100 μM, 300 μL; intraperitoneal injection; single dose; imaging performed 5 min or 1 h post-administration) enables sensitive and specific visual imaging of tiny peritoneal ovarian cancer metastases with a diameter <1 mm in 7 distinct mouse models, and its signal brightness is sufficient to support naked-eye and endoscopic detection (imaging with a blue-green filter, Ex=470-510 nm; Em=530 nm)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/cAJcl-nu/nu nude mice with Ovarian cancer (female, 7-8 weeks old)[1]
Dosage: 300 μL of 100 μM
Administration: i.p.; single dose
Result: Specifically visualized peritoneal metastases as small as <1 mm in diameter in all 7 ovarian cancer cell line models at 5 min post-administration.
Enhanced tumor nodule fluorescence sufficiently for naked-eye detection at 1 h post-administration.
Suppressed tumor nodule fluorescence when co-treated with a β-galactosidase inhibitor.
Successfully visualized metastases via real-time fluorescence endoscopy.
Molecular Weight

561.50

Formula

C28H26F3NO8
CAS No.
SMILES

FC(F)(F)CNC1=CC=C2C3(C4=CC=CC=C4CO3)C5=CC=C(C=C5OC2=C1)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO
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Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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HMRef-βGal Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HMRef-βGal2163840-29-5Fluorescent DyeGlycosidaseperitoneal metastatic tumoursOVCAR3ovarian cancer cellsOVK18SKOV3mouse modelsOVCAR4SHIN3OVCAR5β-galactosidaseInhibitorinhibitorinhibit

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