beta-Galactose dehydrogenase
Based on 1 Customer Validation
Beta-galactose dehydrogenase is a selective catalyst for β-galactose. Under pH 8.6 conditions, beta-galactose dehydrogenase catalyzes the oxidation of β-galactose, produced by the hydrolysis of lactose by β-galactosidase, with nicotinamide adenine dinucleotide (NAD) to produce reduced nicotinamide adenine dinucleotide (NADH). Beta-galactose dehydrogenase specifically mediates this oxidation reaction for the quantitative detection of the substrate, used in the analysis of lactose concentration in samples such as breast milk.
For research use only. We do not sell to patients.
- CAS No.: 9028-54-0
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
-
Biological Activity
-
Chemical Information
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
In quantitative experiments to determine lactose concentration in breast milk (colostrum and mature milk) and cow's milk formula, beta-galactosidase catalyzes the hydrolysis of lactose to β-galactose, which then undergoes an oxidation reaction with nicotinamide adenine dinucleotide (NAD) catalyzed by beta-galactose dehydrogenase at pH 8.6, producing reduced nicotinamide adenine dinucleotide (NADH). The lactose concentration in the sample can be accurately calculated by measuring the absorbance of NADH at 340 nm[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 9028-54-0
-
Appearance Liquid
-
Color Colorless to light yellow
-
SMILES
[b-Galactose dehydrogenase]
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
-
Data Sheet (265 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
References
[1]. Fleischmann WD, et al. Immobilisierung von beta-Galactosedehydrogenase; Verwendung zur enzymatischen Analyse von Galactose [Immobilization of beta-galactose dehydrogenase application for enzymatic analysis of galactose]. Mikrochim Acta. 1976;(3-4 Pt 2):443-8. German. [Content Brief]
[2]. Yuen JW, et al. Nutritional and immunological characteristics of fresh and refrigerated stored human milk in Hong Kong: a pilot study. Clin Chim Acta. 2012 Oct 9;413(19-20):1549-54. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz