beta-Galactose dehydrogenase
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Beta-galactose dehydrogenase is a selective catalyst for β-galactose. Under pH 8.6 conditions, beta-galactose dehydrogenase catalyzes the oxidation of β-galactose, produced by the hydrolysis of lactose by β-galactosidase, with nicotinamide adenine dinucleotide (NAD) to produce reduced nicotinamide adenine dinucleotide (NADH). Beta-galactose dehydrogenase specifically mediates this oxidation reaction for the quantitative detection of the substrate, used in the analysis of lactose concentration in samples such as breast milk.
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 9028-54-0
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
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生物活性
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化学情報
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
体外実験
In quantitative experiments to determine lactose concentration in breast milk (colostrum and mature milk) and cow's milk formula, beta-galactosidase catalyzes the hydrolysis of lactose to β-galactose, which then undergoes an oxidation reaction with nicotinamide adenine dinucleotide (NAD) catalyzed by beta-galactose dehydrogenase at pH 8.6, producing reduced nicotinamide adenine dinucleotide (NADH). The lactose concentration in the sample can be accurately calculated by measuring the absorbance of NADH at 340 nm[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 9028-54-0
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性状 Liquid
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Color Colorless to light yellow
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SMILES
[b-Galactose dehydrogenase]
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
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データシート (265 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Fleischmann WD, et al. Immobilisierung von beta-Galactosedehydrogenase; Verwendung zur enzymatischen Analyse von Galactose [Immobilization of beta-galactose dehydrogenase application for enzymatic analysis of galactose]. Mikrochim Acta. 1976;(3-4 Pt 2):443-8. German. [Content Brief]
[2]. Yuen JW, et al. Nutritional and immunological characteristics of fresh and refrigerated stored human milk in Hong Kong: a pilot study. Clin Chim Acta. 2012 Oct 9;413(19-20):1549-54. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
beta-Galactose dehydrogenase 関連分類
HY-P3185 関連分類
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