beta-Galactose dehydrogenase 

Beta-galactose dehydrogenase is a selective catalyst for β-galactose. Under pH 8.6 conditions, beta-galactose dehydrogenase catalyzes the oxidation of β-galactose, produced by the hydrolysis of lactose by β-galactosidase, with nicotinamide adenine dinucleotide (NAD) to produce reduced nicotinamide adenine dinucleotide (NADH). Beta-galactose dehydrogenase specifically mediates this oxidation reaction for the quantitative detection of the substrate, used in the analysis of lactose concentration in samples such as breast milk^[1][2].

In quantitative experiments to determine lactose concentration in breast milk (colostrum and mature milk) and cow's milk formula, beta-galactosidase catalyzes the hydrolysis of lactose to β-galactose, which then undergoes an oxidation reaction with nicotinamide adenine dinucleotide (NAD) catalyzed by beta-galactose dehydrogenase at pH 8.6, producing reduced nicotinamide adenine dinucleotide (NADH). The lactose concentration in the sample can be accurately calculated by measuring the absorbance of NADH at 340 nm^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9028-54-0

Liquid

Colorless to light yellow

[b-Galactose dehydrogenase]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Fleischmann WD, et al. Immobilisierung von beta-Galactosedehydrogenase; Verwendung zur enzymatischen Analyse von Galactose [Immobilization of beta-galactose dehydrogenase application for enzymatic analysis of galactose]. Mikrochim Acta. 1976;(3-4 Pt 2):443-8. German.  [Content Brief]

  [2]. Yuen JW, et al. Nutritional and immunological characteristics of fresh and refrigerated stored human milk in Hong Kong: a pilot study. Clin Chim Acta. 2012 Oct 9;413(19-20):1549-54.  [Content Brief]