ストロファンチジン (Synonyms: Strophanthidin)

Name: Strophanthidin
Brand: MedChemExpress
SKU: HY-114252
Rating: 4.5 (1 reviews)

製品番号: HY-114252 純度: 98.71%: Data Sheet SDS COA 取扱説明書
FAQs
Technical Support

Strophanthidin is a naturally available cardiac glycoside. Strophanthidin 0.1 and 1 nmol/L increases and 1~100 μmol/L inhibits the Na+/K+-ATPase activities, but Strophanthidin 10 and 100 nmol/L does not affect Na+/K+-ATPase activities in cardiac sarcolemmal. Strophanthidin increases both diastolic and systolic intracellular Ca²⁺ concentration.

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研究用途以外に使用した場合、当社は一切の責任を負いかねます。

ストロファンチジン 構造式

CAS 番号 : 66-28-4

容量	価格（税別）	在庫状況	数量
Solution
10 mM * 1 mL in Ethanol	USD 55	在庫あり	Estimated Time of Arrival: December 31
Solid
5 mg	$50	在庫あり	Estimated Time of Arrival: December 31
10 mg	$80	在庫あり	Estimated Time of Arrival: December 31
25 mg	$150	在庫あり	Estimated Time of Arrival: December 31
50 mg		お問い合わせ
100 mg		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

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Based on 1 publication(s) in Google Scholar

Other Forms of Strophanthidin:

Strophanthidin (Standard) お問い合わせ

顧客検証

•Int J Surg. 2025 Nov 5. [Abstract]

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•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

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製品説明

Strophanthidin is a naturally available cardiac glycoside^[1]. Strophanthidin 0.1 and 1 nmol/L increases and 1~100 μmol/L inhibits the Na+/K+-ATPase activities, but Strophanthidin 10 and 100 nmol/L does not affect Na+/K+-ATPase activities in cardiac sarcolemmal^[2]. Strophanthidin increases both diastolic and systolic intracellular Ca²⁺ concentration^[3].

IC₅₀ & Target

Na+/K+-ATPase^[2]

Cellular Effect

Cell Line	Type	Value	Description	References
1A9	ED₅₀	0.15 6 Compound: 19	Cytotoxicity against human 1A9 cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human 1A9 cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 24582402]
1A9	ED₅₀	0.15 6 Compound: 19	Cytotoxicity against human 1A9 cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human 1A9 cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 24582402]
1A9	ED₅₀	0.15 6 Compound: 19	Cytotoxicity against human 1A9 cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human 1A9 cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 24582402]
A549	ED₅₀	0.11 6 Compound: 19	Cytotoxicity against human A549 cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human A549 cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 24582402]
A549	ED₅₀	0.11 6 Compound: 19	Cytotoxicity against human A549 cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human A549 cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 24582402]
A549	ED₅₀	0.11 6 Compound: 19	Cytotoxicity against human A549 cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human A549 cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 24582402]
CAKI-1	ED₅₀	0.14 6 Compound: 19	Cytotoxicity against human CAKI-1 cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human CAKI-1 cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 24582402]
CAKI-1	ED₅₀	0.14 6 Compound: 19	Cytotoxicity against human CAKI-1 cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human CAKI-1 cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 24582402]
CAKI-1	ED₅₀	0.14 6 Compound: 19	Cytotoxicity against human CAKI-1 cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human CAKI-1 cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 24582402]
KB	ED₅₀	0.44 6 Compound: strophanthidin	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 4031899]
KB	ED₅₀	0.61 6 Compound: 19	Cytotoxicity against human KB cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human KB cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 24582402]
MCF7	ED₅₀	0.38 6 Compound: 19	Cytotoxicity against human MCF7 cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human MCF7 cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 24582402]
KB	ED₅₀	0.61 6 Compound: 19	Cytotoxicity against human KB cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human KB cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 24582402]
PC-3	ED₅₀	0.17 6 Compound: 19	Cytotoxicity against human PC3 cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human PC3 cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 24582402]
MCF7	ED₅₀	0.38 6 Compound: 19	Cytotoxicity against human MCF7 cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human MCF7 cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 24582402]
PC-3	ED₅₀	0.17 6 Compound: 19	Cytotoxicity against human PC3 cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human PC3 cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 24582402]
SK-MEL-2	ED₅₀	0.13 6 Compound: 19	Cytotoxicity against human SK-MEL-2 cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human SK-MEL-2 cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 24582402]
KB	ED₅₀	0.44 6 Compound: strophanthidin	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 4031899]
SK-MEL-2	ED₅₀	0.13 6 Compound: 19	Cytotoxicity against human SK-MEL-2 cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human SK-MEL-2 cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 24582402]
U-251	EC₅₀	2 3 Compound: 10	Inhibition of HIF1 activation in human U251 cells stably transfected in pGL2-TK-HRE plasmid under hypoxic condition after 16 to 24 hrs by luciferase reporter gene assay Inhibition of HIF1 activation in human U251 cells stably transfected in pGL2-TK-HRE plasmid under hypoxic condition after 16 to 24 hrs by luciferase reporter gene assay	[PMID: 19405508]
KB	ED₅₀	0.61 6 Compound: 19	Cytotoxicity against human KB cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human KB cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 24582402]
U-87MG ATCC	ED₅₀	0.72 6 Compound: 19	Cytotoxicity against human U87MG cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human U87MG cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 24582402]
U-251	EC₅₀	2 3 Compound: 10	Inhibition of HIF1 activation in human U251 cells stably transfected in pGL2-TK-HRE plasmid under hypoxic condition after 16 to 24 hrs by luciferase reporter gene assay Inhibition of HIF1 activation in human U251 cells stably transfected in pGL2-TK-HRE plasmid under hypoxic condition after 16 to 24 hrs by luciferase reporter gene assay	[PMID: 19405508]
MCF7	ED₅₀	0.38 6 Compound: 19	Cytotoxicity against human MCF7 cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human MCF7 cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 24582402]
U-87MG ATCC	ED₅₀	0.72 6 Compound: 19	Cytotoxicity against human U87MG cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human U87MG cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 24582402]
PC-3	ED₅₀	0.17 6 Compound: 19	Cytotoxicity against human PC3 cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human PC3 cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 24582402]
SK-MEL-2	ED₅₀	0.13 6 Compound: 19	Cytotoxicity against human SK-MEL-2 cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human SK-MEL-2 cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 24582402]
U-251	EC₅₀	2 3 Compound: 10	Inhibition of HIF1 activation in human U251 cells stably transfected in pGL2-TK-HRE plasmid under hypoxic condition after 16 to 24 hrs by luciferase reporter gene assay Inhibition of HIF1 activation in human U251 cells stably transfected in pGL2-TK-HRE plasmid under hypoxic condition after 16 to 24 hrs by luciferase reporter gene assay	[PMID: 19405508]
U-87MG ATCC	ED₅₀	0.72 6 Compound: 19	Cytotoxicity against human U87MG cells assessed as growth inhibition after 72 hrs by SRB assay Cytotoxicity against human U87MG cells assessed as growth inhibition after 72 hrs by SRB assay	[PMID: 24582402]

体外実験

Strophanthidin (0~10 μM; 24 hours; MCF-7, A549, and HepG2 cells) is effective at suppressing the growth of cancer cells and has no toxicity in normal cells^[1].
Strophanthidin (0.5 to 500 μM; PBMCs) does not show significant cytotoxicity in PBMCs. Strophanthidin (2 μM; MCF-7 cells) can arrest cell cycle at the G2/M phase^[1].
Strophanthidin (MCF-7, A549, and HepG2 cells) is effective at suppressing the growth of cancer cells and has no toxicity in normal cells. Strophanthidin (MCF-7, A549, and HepG2 cells) inhibits the expression of checkpoint and cyclin-dependent kinases in three cancer cells compared to untreated controls. Strophanthidin can modulate the protein localization from the nucleus to the membrane as well as to the cytoplasm. Strophanthidin is a monosaccharide cardiac glycoside with one aglycone portion and without any sugar unit. Strophanthidin induces apoptosis by the attenuation of multiple biochemical signaling pathways and by arresting cell cycle at the G2/M phase through p53-dependent and p53-independent mechanisms^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	MCF-7, A549, and HepG2 cells
Concentration:	0~10 μM
Incubation Time:	24 hours
Result:	Inhibited the proliferation in three different cancer cells.

分子量

404.50

分子式

C₂₃H₃₂O₆

CAS 番号

66-28-4

Appearance

Solid

Color

White to off-white

SMILES

O=C[C@@]12[C@]3([H])[C@](CC[C@@]1(C[C@@H](O)CC2)O)([H])[C@@]4([C@@](C)([C@@H](C5=CC(OC5)=O)CC4)CC3)O

Structure Classification

Steroids

Initial Source

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶剤 & 溶解度

体外:

Ethanol : 25 mg/mL (61.80 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4722 mL	12.3609 mL	24.7219 mL
5 mM	0.4944 mL	2.4722 mL	4.9444 mL
10 mM	0.2472 mL	1.2361 mL	2.4722 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.18 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.18 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.18 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

純度とドキュメンテーション

純度: 98.71%

Select Batch:

データシート (282 KB) SDS (480 KB)

COA (258 KB) HNMR (272 KB) RP-HPLC (215 KB) MS (71 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Reddy D, et al. Strophanthidin Attenuates MAPK, PI3K/AKT/mTOR, and Wnt/β-Catenin Signaling Pathways in Human Cancers. Front Oncol. 2020;9:1469. Published 2020 Jan 17. [Content Brief]

[2]. Su SW, et al. Relationship between cardiotonic effects and inhibition on cardiac sarcolemmal Na+,K+-ATPase of strophan-thidin at low concentrations. Acta Pharmacol Sin. 2003;24(11):1103-1107. [Content Brief]

[3]. Bennett DL, et al. Strophanthidin-induced gain of Ca2+ occurs during diastole and not systole in guinea-pig ventricular myocytes. Pflugers Arch. 1999;437(5):731-736. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol	1 mM	2.4722 mL	12.3609 mL	24.7219 mL	61.8047 mL
	5 mM	0.4944 mL	2.4722 mL	4.9444 mL	12.3609 mL
	10 mM	0.2472 mL	1.2361 mL	2.4722 mL	6.1805 mL
	15 mM	0.1648 mL	0.8241 mL	1.6481 mL	4.1203 mL
	20 mM	0.1236 mL	0.6180 mL	1.2361 mL	3.0902 mL
	25 mM	0.0989 mL	0.4944 mL	0.9889 mL	2.4722 mL
	30 mM	0.0824 mL	0.4120 mL	0.8241 mL	2.0602 mL
	40 mM	0.0618 mL	0.3090 mL	0.6180 mL	1.5451 mL
	50 mM	0.0494 mL	0.2472 mL	0.4944 mL	1.2361 mL
	60 mM	0.0412 mL	0.2060 mL	0.4120 mL	1.0301 mL

Strophanthidin Related Classifications

Cardiovascular Disease

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Strophanthidin66-28-4Na+/K+ ATPaseSodium potassium pumpnaturallyavailablecardiacglycosidesarcolemmaldiastolicsystolicInhibitorinhibitorinhibit

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ストロファンチジン (Synonyms: Strophanthidin)

カスタマーレビュー

Other Forms of Strophanthidin:

ストロファンチジン 関連抗体

顧客検証

おすすめ

•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

生物活性

純度とドキュメンテーション

参考文献

カスタマーレビュー

ストロファンチジン 関連抗体

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Strophanthidin Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

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ストロファンチジン関連抗体

ストロファンチジン関連抗体