VBIT-12

Name: VBIT-12
Brand: MedChemExpress
SKU: HY-135885
Rating: 5.0 (7 reviews)

Cat. No.: HY-135885 Pureza: 98.87%: Ficha de datos
COA

SDS
Instrucciones de manejo
FAQs
Technical Support

VBIT-12 is a potent VDAC1 inhibitor. VBIT-12 directly interacts with VDAC1 and prevents VDAC1 oligomerization, and thus inhibits apoptotic action of VDAC1.

Para uso exclusivo en investigación. No vendemos a pacientes.

No. CAS : 2089227-65-4

Tamaño	Stock	Cantidad
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid
5 mg	En stock	Estimated Time of Arrival: December 31
10 mg	En stock	Estimated Time of Arrival: December 31
25 mg	En stock	Estimated Time of Arrival: December 31
50 mg	En stock	Estimated Time of Arrival: December 31
100 mg	En stock	Estimated Time of Arrival: December 31
200 mg	Obtener un presupuesto
500 mg	Obtener un presupuesto

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Revisión del cliente

Based on 7 publication(s) in Google Scholar

7 Publications Citing Use of MCE VBIT-12

•Discov Oncol. 2025 Nov 19;16(1):2129. [Abstract]

Others

Cell Proliferation/Viability Assay

: VBIT-12 purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Apr 4;11(14):eadr6415. [Abstract]; The role of mitochondrial membrane pores in mtDNA release was analyzed. Under specified conditions, 24 hours after transfection, cells were treated for 4 hours with 10 μM chloroquine, 25 μM VBIT-12 (to inhibit VDAC1), or 100 μM V5 peptide (to inhibit BAX oligomerization).

: VBIT-12 purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2025 Oct 25.; Inhibition of VDAC1 oligomerization maintained RPE function in vitro. ARPE-19 cells were pretreated with 10 μM VBIT-12 for 2 h, followed by exposure to 10 mM NaIO3 for 24 h. Western blots of EGS-treated VDAC1 monomers and oligomers were obtained from ARPE-19 cells in the control group, NaIO3-induced group, and NaIO3 and VBIT-12 pretreated groups.

: VBIT-12 purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2025 Oct 25.; Inhibition of VDAC1 oligomerization maintained RPE function in vitro. ARPE-19 cells were pretreated with 10 μM VBIT-12 for 2 h, followed by exposure to 10 mM NaIO3 for 24 h. Representative immunofluorescence images of ZO-1 from each group are shown. Cell nuclei were counterstained with DAPI. The figure shows the quantitative analysis results of ZO-1 fluorescence intensity.

: VBIT-12 purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2025 Oct 25.; Inhibition of VDAC1 oligomerization maintained RPE function in vitro. ARPE-19 cells were pretreated with 10 μM VBIT-12 for 2 hours, followed by exposure to 10 mM NaIO3 for 24 hours. Cell viability was assessed using the CCK-8 assay in each group.

: VBIT-12 purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2025 Oct 25.; Co-IP assay demonstrating the physical association of STING with major PANoptosome components (NLRP3, Caspase-8, and RIPK3) in NaIO3-induced ARPE-19 cells with or without VBIT-12 intervention.

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Ver todos los productos específicos de isoformas VDAC:

Ver todas las isoformas

VDAC1 VDAC2 VDAC3

Actividad biológica
Pureza y Documentación
Referencias
Revisión del cliente

Descripciòn

VBIT-12 is a potent VDAC1 inhibitor. VBIT-12 directly interacts with VDAC1 and prevents VDAC1 oligomerization, and thus inhibits apoptotic action of VDAC1^[1].

IC₅₀ & Target

VDAC1

In Vitro

VBIT-12 inhibits VDAC1 oligomerization, hexokinase (HK) detachment and apoptotic cell death^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Peso molecular

417.50

Fòrmula

C₂₅H₂₇N₃O₃

No. CAS

2089227-65-4

Appearance

Solid

Color

White to off-white

SMILES

O=C(O)CNC(C1(NC2=CC=CC=C2)CCN(CC3=C4C=CC=CC4=CC=C3)CC1)=O

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvente y solubilidad

In Vitro:

DMSO : 250 mg/mL (598.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3952 mL	11.9760 mL	23.9521 mL
5 mM	0.4790 mL	2.3952 mL	4.7904 mL
10 mM	0.2395 mL	1.1976 mL	2.3952 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (4.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureza y Documentación

Purity: 98.87%

Select Batch:

Ficha de datos (276 KB) SDS (537 KB)

COA (253 KB) HNMR (305 KB) RP-HPLC (246 KB) LCMS (87 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Shoshan-Barmatz V, et al. VDAC1, mitochondrial dysfunction, and Alzheimer's disease. Pharmacol Res. 2018 May;131:87-101. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.3952 mL	11.9760 mL	23.9521 mL	59.8802 mL
	5 mM	0.4790 mL	2.3952 mL	4.7904 mL	11.9760 mL
	10 mM	0.2395 mL	1.1976 mL	2.3952 mL	5.9880 mL
	15 mM	0.1597 mL	0.7984 mL	1.5968 mL	3.9920 mL
	20 mM	0.1198 mL	0.5988 mL	1.1976 mL	2.9940 mL
	25 mM	0.0958 mL	0.4790 mL	0.9581 mL	2.3952 mL
	30 mM	0.0798 mL	0.3992 mL	0.7984 mL	1.9960 mL
	40 mM	0.0599 mL	0.2994 mL	0.5988 mL	1.4970 mL
	50 mM	0.0479 mL	0.2395 mL	0.4790 mL	1.1976 mL
	60 mM	0.0399 mL	0.1996 mL	0.3992 mL	0.9980 mL
	80 mM	0.0299 mL	0.1497 mL	0.2994 mL	0.7485 mL
	100 mM	0.0240 mL	0.1198 mL	0.2395 mL	0.5988 mL

VBIT-12 Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

VBIT-122089227-65-4VBIT12VBIT 12VDACVoltage-dependent anion channelVDAC1CNSpsychoticdisordersmitochondrialapoptosisoligomerizationInhibitorinhibitorinhibit

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VBIT-12

Revisión del cliente

VBIT-12 Related Antibodies

7 Publications Citing Use of MCE VBIT-12

Recomendaciones destacadas

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Ver todos los productos específicos de isoformas VDAC:

Actividad biológica

Pureza y Documentación

Referencias

Revisión del cliente

VBIT-12 Related Antibodies

Calculadora de molaridad

Calculadora de dilución

Complete Stock Solution Preparation Table

VBIT-12 Related Classifications

Keywords:

Productos vistos recientemente:

Revisión del cliente

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SAFETY DATA SHEET (SDS)

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