2,4-Diamino-6-hydroxypyrimidine
Based on 7 publication(s) in Google Scholar
2,4-Diamino-6-hydroxypyrimidine is a specific GTP cyclohydrolase I inhibitor (the rate-limiting enzyme in de novo pterin synthesis). 2,4-Diamino-6-hydroxypyrimidine blocks Tetrahydrobiopterin (BH4) synthesis and suppresses NO production.
For research use only. We do not sell to patients.
- Purity: 99.82%
- CAS No.: 56-06-4
- Formula: C4H6N4O
- Molecular Weight:126.12
-
Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
HY-15763
Erastin
-
HY-108325
Brequinar
-
HY-107383
Tetrahydrobiopterin
-
HY-136057
iFSP1
-
HY-14655
Sulfasalazine
-
HY-100218A
RSL3
-
HY-N0111
Coenzyme Q10
-
HY-115510
SPR inhibitor 3
-
HY-100954R
2,4-Diamino-6-hydroxypyrimidine (Standard)
-
HY-100965
Diphenyleneiodonium chloride
-
HY-102015
6-Biopterin
-
HY-N0091
Hypoxanthine
Publications Citing Use of MedChemExpress (MCE) 2,4-Diamino-6-hydroxypyrimidine
More- Nat Commun. 2026 Jan 20;17(1):1800. [Abstract]
- Mol Ther. 2026 Jan 29:S1525-0016(26)00031-6. [Abstract]
- Genome Biol. 2025 Sep 4;26(1):267. [Abstract]
- Free Radic Biol Med. 2025 Apr 12:S0891-5849(25)00225-4. [Abstract]
- Front Cell Dev Biol. 2022 Feb 10:10:810327. [Abstract]
- bioRxiv. 2026 Mar 31.
- SSRN. 2025 Aug 11.
Biological Activity
GTP cyclohydrolase I[1]
2,4-Diamino-6-hydroxypyrimidine (DAHP) exhibits a dose-dependent reduction in NO production in both chicken peritoneal macrophages (PECs) and the avian MC29 virus-transformed macrophage cell line, HD11[1].
2,4-Diamino-6-hydroxypyrimidine (DAHP; 5 mM; 24 hours) treatment significantly reduces both BH4 and VCAM-1 levels during the time course in HUVEC cells. 2,4-Diamino-6-hydroxypyrimidine reduces VCAM-1 and GTPCH protein synthesis at least partially via suppressing the NF-κB level in the nucleus of HUVEC[2].
2,4-Diamino-6-hydroxypyrimidine (DAHP) is first identified as an inhibitor of the biopterin-dependent growth of the protozoan Crithidia fasciculate[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 56-06-4
-
Appearance Solid
-
Molecular Weight 126.12
-
Formula C4H6N4O
-
Color Off-white to light yellow
-
SMILES
O=C1N=C(N)NC(N)=C1
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (7)
-
Journal Impact Factor
-
Most Recent
-
Nat Commun
TET1 as a master regulator controlling GPX4-dependent and -independent ferroptosis surveillance in acute myeloid leukemia. [Abstract]2026 Jan 20;17(1):1800. PMID: 41559050 -
Mol Ther
Dysregulation of Serpinb6a-Gch1 axis contributes to DFNB91 deafness that is amendable to gene therapies. [Abstract]2026 Jan 29:S1525-0016(26)00031-6. PMID: 41612696 -
Genome Biol
Artemisinin alleviates Parkinson's disease by targeting Adcy5-Gch1 axis to trigger a cascade generation of BH4 and dopamine in rats. [Abstract]2025 Sep 4;26(1):267. PMID: 40908481 -
Free Radic Biol Med
Intermedin protects peritubular capillaries by inhibiting eNOS uncoupling through AMPK/GTPCH-I/BH4 pathway and alleviate CKD following AKI. [Abstract]2025 Apr 12:S0891-5849(25)00225-4. PMID: 40228707 -
Front Cell Dev Biol
Blockade of GCH1/BH4 Axis Activates Ferritinophagy to Mitigate the Resistance of Colorectal Cancer to Erastin-Induced Ferroptosis. [Abstract]2022 Feb 10:10:810327. PMID: 35223839 -
-
Solvent & Solubility
DMSO : 16.67 mg/mL (132.18 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 10 mg/mL (79.29 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.67 mg/mL (13.24 mM); Clear solution
This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.67 mg/mL (13.24 mM); Clear solution
This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 6.5 mg/mL (51.54 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (277 KB)
-
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
-
Handling Instructions (2659 KB)
References
[1]. Sung YJ, et al. 2,4-Diamino-6-hydroxypyrimidine, an inhibitor of GTP cyclohydrolase I, suppresses nitric oxide production by chicken macrophages. Int J Immunopharmacol. 1994 Feb;16(2):101-8. [Content Brief]
[2]. Kazuhisa Ikemoto, et al. 2,4-Diamino-6-hydroxypyrimidine (DAHP) suppresses cytokine-induced VCAM-1 expression on the cell surface of human umbilical vein endothelial cells in a BH(4)-independent manner. Biochim Biophys Acta. Jul-Aug 2008;1780(7-8):960-5. [Content Brief]
[3]. T Kobayashi, et al. Gastrointestinal serotonin: depletion due to tetrahydrobiopterin deficiency induced by 2,4-diamino-6-hydroxypyrimidine administration. J Pharmacol Exp Ther. 1991 Feb;256(2):773-9. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 7.9290 mL | 39.6448 mL | 79.2896 mL | 198.2239 mL |
| 5 mM | 1.5858 mL | 7.9290 mL | 15.8579 mL | 39.6448 mL | |
| 10 mM | 0.7929 mL | 3.9645 mL | 7.9290 mL | 19.8224 mL | |
| 15 mM | 0.5286 mL | 2.6430 mL | 5.2860 mL | 13.2149 mL | |
| 20 mM | 0.3964 mL | 1.9822 mL | 3.9645 mL | 9.9112 mL | |
| 25 mM | 0.3172 mL | 1.5858 mL | 3.1716 mL | 7.9290 mL | |
| 30 mM | 0.2643 mL | 1.3215 mL | 2.6430 mL | 6.6075 mL | |
| 40 mM | 0.1982 mL | 0.9911 mL | 1.9822 mL | 4.9556 mL | |
| 50 mM | 0.1586 mL | 0.7929 mL | 1.5858 mL | 3.9645 mL | |
| 60 mM | 0.1321 mL | 0.6607 mL | 1.3215 mL | 3.3037 mL | |
| DMSO | 80 mM | 0.0991 mL | 0.4956 mL | 0.9911 mL | 2.4778 mL |
| 100 mM | 0.0793 mL | 0.3964 mL | 0.7929 mL | 1.9822 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
2,4-Diamino-6-hydroxypyrimidine Related Classifications
HY-100954 Related Classifications
Powered by Bioz