2. Metabolic Enzyme/Protease Protein Tyrosine Kinase/RTK
  3. E1/E2/E3 Enzyme TAM Receptor
  4. 2-D08

2-D08 is a cell permeable, mechanistically unique inhibitor of protein SUMOylation. 2-D08 also inhibits Axl with an IC50 of 0.49 nM.

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CAS 番号 : 144707-18-6

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 72 在庫あり
Solution
10 mM * 1 mL in DMSO USD 72 在庫あり
Solid
5 mg $66 在庫あり
10 mg $110 在庫あり
25 mg $231 在庫あり
50 mg $374 在庫あり
100 mg $585 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 12 publication(s) in Google Scholar

2-D08 関連抗体

Top Publications Citing Use of Products

    2-D08 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2022 Sep 3;13(1):5204.  [Abstract]

    Immunoblotting of 293 T cells transfected with plasmids of Flag-ACE2 and HA-SUMO3 in the presence of 2-D08 (200 μM; 24 h), ML-792 (10 μM), GA (5 μM), or TAK-981 (5 μM). 2-D08 largely decreased the SUMOylation of ACE2.

    2-D08 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2022 Sep 3;13(1):5204.  [Abstract]

    Protein abundance and mRNA level of ACE2 in Calu-3 cells treated with 2-D08 (100-300 μM; 24h). Protein levels of ACE2 were gradually reduced upon 2-D08 treatments in a dose-dependent manner.

    2-D08 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2022 Sep 3;13(1):5204.  [Abstract]

    293 T cells were transfected with plasmids encoding Flag-ACE2 and HA-TOLLIP, and treatedwith indicated concentrations of 2-D08 (100-200 μM; 24 h). The cells cultured with Baf A1 (0.2 μM) for 6 h were immunoprecipitated with α-FLAG M2 beads and immunoblotted with anti-HA. ACE2-TOLLIP association was increased upon 2-D08 treatment.

    2-D08 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2022 Sep 3;13(1):5204.  [Abstract]

    GSEA analysis of differentially expressed genes (DEGs) in Calu-3 cells infected with SARS-CoV-2 (MOI = 0.5) for 24 h with or without 2-D08 (100 μM) treatment.

    2-D08 purchased from MedChemExpress. Usage Cited in: Life Sci. 2020 Aug 15;255:117859.  [Abstract]

    The expression levels of TGF-β/SMAD and HIF-1 signaling pathways-related proteins, including Col III, Smad2, Smad3, Smad4 and HIF-1α are measured by western blotting in HFFs and HKFs. The band intensity ratio of the TGF-β/SMAD and HIF-1 signaling related protein to reference protein is significantly decreased in the 2-D08 group.

    TAM Receptor アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Axl Mer Tyro3

    • 生物活性

    • プロトコル

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    2-D08 is a cell permeable, mechanistically unique inhibitor of protein SUMOylation. 2-D08 also inhibits Axl with an IC50 of 0.49 nM.

    IC50 & Target

    Axl

     

    体外実験

    2-D08 inhibits sumoylation by preventing transfer of SUMO from the UBC9-SUMO thioester to the substrate[1]. 2-D08 decreases the ratio of p-Axl to t-Axl in a dose-dependent manner. Suppression of Axl kinase activity by 2D08 disrupts the cytoskeleton and actin filaments with re-organization at cellular junctions[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    2-D08 関連抗体
    分子量

    270.24

    分子式

    C15H10O5
    CAS 番号
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C1C=C(C2=CC=C(O)C(O)=C2O)OC3=CC=CC=C13
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
    溶剤 & 溶解度
    体外: 

    DMSO : 66.67 mg/mL (246.71 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.7004 mL 18.5021 mL 37.0041 mL
    5 mM 0.7401 mL 3.7004 mL 7.4008 mL
    10 mM 0.3700 mL 1.8502 mL 3.7004 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
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    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

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    体積 （終了）

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    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: 5 mg/mL (18.50 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

      This protocol yields a clear solution of 5 mg/mL.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.37%

    COA (250 KB) HNMR (268 KB) RP-HPLC (255 KB) LCMS (235 KB)

    取扱説明書 (2659 KB)
    参考文献
    細胞実験
    [2]

    Human lung multi-potent cells at passage 5 are incubated with DMEM+0.5% BSA+penicillin/streptomycin containing either 0.1% DMSO (vehicle) or 2D08 (10 μM) on Permanox culture slides for 6 days. Cells are fixed with 4% PFA for 30 min and then blocked and permeabilized with 10% goat serum and 0.3% Triton-X 100 in PBS for 30 min[2].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    動物実験
    [3]

    Mice[3]

    Eight- to 10-week-old mice (both male and female) are used for the study. A 5 cm-long glass pipette is buffered with Mineral Oil and then attached to the injection apparatus to take up 10 μL of 2-D08 (30 μM) or NaCl[3].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.7004 mL 18.5021 mL 37.0041 mL 92.5104 mL
    5 mM 0.7401 mL 3.7004 mL 7.4008 mL 18.5021 mL
    10 mM 0.3700 mL 1.8502 mL 3.7004 mL 9.2510 mL
    15 mM 0.2467 mL 1.2335 mL 2.4669 mL 6.1674 mL
    20 mM 0.1850 mL 0.9251 mL 1.8502 mL 4.6255 mL
    25 mM 0.1480 mL 0.7401 mL 1.4802 mL 3.7004 mL
    30 mM 0.1233 mL 0.6167 mL 1.2335 mL 3.0837 mL
    40 mM 0.0925 mL 0.4626 mL 0.9251 mL 2.3128 mL
    50 mM 0.0740 mL 0.3700 mL 0.7401 mL 1.8502 mL
    60 mM 0.0617 mL 0.3084 mL 0.6167 mL 1.5418 mL
    80 mM 0.0463 mL 0.2313 mL 0.4626 mL 1.1564 mL
    100 mM 0.0370 mL 0.1850 mL 0.3700 mL 0.9251 mL
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    2-D08 Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    2-D08144707-18-6E1/E2/E3 EnzymeTAM ReceptorE1 activating enzymeE2 conjugating enzymeE3 ligating enzymeUbiquitin activating enzymeUbiquitin conjugating enzymeUbiquitin ligaseTyro3AxlMerInhibitorinhibitorinhibit

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    製品名:
    2-D08
    製品番号:
    HY-114166
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