2. Metabolic Enzyme/Protease Anti-infection
  3. Thrombin Influenza Virus Ser/Thr Protease
  4. AEBSF hydrochloride

AEBSF (hydrochloride)  (Synonyms: 4-(2-アミノエチル)ベンゼンスルホニルフルオリド塩酸塩)

製品番号: HY-12821 純度: 99.87%
COA 取扱説明書 Technical Support

AEBSF hydrochloride is an irreversible inhibitor of serine proteases, such as chymotrypsin, kallikrein, plasmin, thrombin, and trypsin.

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CAS 番号 : 30827-99-7

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 80 在庫あり
Solution
10 mM * 1 mL in DMSO USD 80 在庫あり
Solid
100 mg $73 在庫あり
200 mg $132 在庫あり
500 mg   お問い合わせ  
1 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 18 publication(s) in Google Scholar

AEBSF (hydrochloride) 関連抗体

Top Publications Citing Use of Products

    AEBSF hydrochloride purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2025 Jun 11:161:115005.  [Abstract]

    AEBSF hydrochloride (50-250 μM, 2 h)The concentration of AEBSF hydrochloride and its effects on intestinal barrier. IPEC: intestinal epithelial cell

    AEBSF hydrochloride purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2025 Jun 11:161:115005.  [Abstract]

    The effects of AEBSF hydrochloride (100 μM) on intestinal barrier.

    AEBSF hydrochloride purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2025 Jun 11:161:115005.  [Abstract]

    AEBSF hydrochloride (100 μM). qPCR analyse the expression of autophagyrelated indicators after inhibiting ATF6.

    AEBSF hydrochloride purchased from MedChemExpress. Usage Cited in: Arch Biochem Biophys. 2020 Jul 30;688:108402.  [Abstract]

    Western blotting analysis of the expression level of pATF6, CHOP, Cleaved Caspase12 and Bax in shRNA-Cs1429 and shRNA-Cs1429+AEBSF cells.

    AEBSF hydrochloride purchased from MedChemExpress. Usage Cited in: Arch Biochem Biophys. 2020 Jul 30;688:108402.  [Abstract]

    The proliferation capacity of shRNA-Cs1429 transfected cells, by treatment with AEBSF (200 μmol/l), is the highest among all the groups.

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    製品説明

    AEBSF hydrochloride is an irreversible inhibitor of serine proteases, such as chymotrypsin, kallikrein, plasmin, thrombin, and trypsin.

    体外実験

    AEBSF inhibits the constitutive production of Aβ by directly inhibiting β-secretase in five different human cell lines, both neural and nonneural[1].
    AEBSF, as a serine protease inhibitor, inhibits the lysis of leukemic cells by human macrophages without inhibiting macrophage secretion of TNF-α and IL-1β[2].
    AEBSF also disturbs the growth of blastocysts on endometrial cells and inhibit the adhesion of HeLa cells on HUVECs by altering the protein secretion pattern[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    AEBSF (hydrochloride) 関連抗体
    体内実験

    AEBSF (76.8 mg/kg daily, i.p.) results in prolongation of the survival of mice that have been given a lethal T. gondii infection[3]. AEBSF also reduces airway response and underlying inflammation in cockroach allergen-induced murine model[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    239.69

    分子式

    C8H11ClFNO2S
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=S(C1=CC=C(CCN)C=C1)(F)=O.[H]Cl
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
    溶剤 & 溶解度
    体外: 

    DMSO : 100 mg/mL (417.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : ≥ 100 mg/mL (417.21 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.1721 mL 20.8603 mL 41.7206 mL
    5 mM 0.8344 mL 4.1721 mL 8.3441 mL
    10 mM 0.4172 mL 2.0860 mL 4.1721 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (8.68 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (8.68 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (417.21 mM); Clear solution; Need ultrasonic

    • Protocol 2

      Add each solvent one by one:  Saline

      Solubility: 100 mg/mL (417.21 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション

    純度: 99.90%

    COA (247 KB) HNMR (205 KB) LCMS (113 KB)

    取扱説明書 (2659 KB)
    参考文献
    細胞実験
    [4]

    The HeLa cells suspended in RPMI-1640 media containing 10% FCS are plated into each well of a 96-well microplate (5×103 cells/200 μL/well). After incubation for 24 h at 37°C, cells are treated with different doses of AEBSF (0, 25, 50, 100 μg/mL) for 48 h. Then, 20 μL fresh 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-diphenytetrazoliumromide (MTT) reagent (5 μg/μL) is added into each well, and cells are cultured at 37°C in 5% CO2 for another 4 h. The media are discarded carefully, and 150 μL DMSO is added. Absorbance is read on a microplate reader at dual wavelengths of 540 and 620 nm.

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    動物実験
    [3]

    Mice injected with 2.5×103 parasites are randomLy assigned to one of the treatment groups according to the treatment given: without drugs (control group), vehicle alone (vehicle control group), pyrimethamine alone at different doses, LY311727 alone at different doses, AEBSF alone at different doses or AEBSF 76.8 mg/kg plus pyrimethamine 10 mg/kg. Each treatment group consists of 10 animals. Treatment is initiated 24 h after parasite inoculation and is continued for seven consecutive days. Mouse survival is monitored daily and continued in live mice until 15 days post-infection. All experiments are performed three times and the data shown represent the cumulative results.

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 4.1721 mL 20.8603 mL 41.7206 mL 104.3014 mL
    5 mM 0.8344 mL 4.1721 mL 8.3441 mL 20.8603 mL
    10 mM 0.4172 mL 2.0860 mL 4.1721 mL 10.4301 mL
    15 mM 0.2781 mL 1.3907 mL 2.7814 mL 6.9534 mL
    20 mM 0.2086 mL 1.0430 mL 2.0860 mL 5.2151 mL
    25 mM 0.1669 mL 0.8344 mL 1.6688 mL 4.1721 mL
    30 mM 0.1391 mL 0.6953 mL 1.3907 mL 3.4767 mL
    40 mM 0.1043 mL 0.5215 mL 1.0430 mL 2.6075 mL
    50 mM 0.0834 mL 0.4172 mL 0.8344 mL 2.0860 mL
    60 mM 0.0695 mL 0.3477 mL 0.6953 mL 1.7384 mL
    80 mM 0.0522 mL 0.2608 mL 0.5215 mL 1.3038 mL
    100 mM 0.0417 mL 0.2086 mL 0.4172 mL 1.0430 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    AEBSF hydrochloride Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    AEBSF30827-99-7ThrombinInfluenza VirusSer/Thr ProteaseSerine proteasesSerine endopeptidasesThreonine proteasesInhibitorinhibitorinhibit

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    製品名:
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    製品番号:
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