2. Academic Validation
  3. Cysteine-reactive mitigators of small vessel disease-related NOTCH3 mutants

Cysteine-reactive mitigators of small vessel disease-related NOTCH3 mutants

  • Sci Rep. 2026 Mar 20;16(1):14300. doi: 10.1038/s41598-026-45103-1.
Naw May Pearl Cartee 1 2,  Xiaojie Zhang 1 2,  Soo Jung Lee 1 2,  Michael M Wang 3 4 5
Affiliations

Affiliations

  • 1 Department of Neurology, University of Michigan, 1137 Catherine St., Box 5622, Ann Arbor, MI, 48109-5622, USA.
  • 2 Department of Veterans Affairs, Neurology Service, VA Ann Arbor Healthcare System, Ann Arbor, MI, 48105, USA.
  • 3 Department of Neurology, University of Michigan, 1137 Catherine St., Box 5622, Ann Arbor, MI, 48109-5622, USA. [email protected].
  • 4 Departments of Molecular and Integrative Physiology, 7725 Medical Science Building II, University of Michigan, 1137 Catherine St., Box 5622, Ann Arbor, MI, 48109-5622, USA. [email protected].
  • 5 Department of Veterans Affairs, Neurology Service, VA Ann Arbor Healthcare System, Ann Arbor, MI, 48105, USA. [email protected].
PMID: 41862552 DOI: 10.1038/s41598-026-45103-1
Abstract

Pathogenic alterations in NOTCH3 cause CADASIL, an accelerated and currently untreatable form of cerebrovascular disease. CADASIL mutant NOTCH3, which frequently harbors abnormalities in EGF repeat cysteine number, adopt disulfide dependent abnormal conformations. To seek potential strategies to mitigate the impact of CADASIL mutations on NOTCH3, we investigated whether cysteine-targeting compounds may affect pathological NOTCH3. LSL-NOTCH3, a split luciferase assay that discriminates between benign and pathogenic NOTCH3 conformations, was used to quantify the capacity of 21 small molecule compounds to restore NOTCH3 reporter activity. We assayed the activity of each of the small molecules on 16 different pathogenic mutants distributed over three different regions of NOTCH3. Ten of 21 compounds had statistically significant effects on at least one mutant NOTCH3. Five of 21 compounds had mitigating effects on a majority of mutant NOTCH3 in three regions of the protein, with disulfiram and auranofin targeting the most favorable range of mutants. These findings support the concept that cysteine-targeting can potentially mitigate the effects of a broad range of NOTCH3 pathogenic mutants and provide an impetus to investigate whether cysteine-targeting strategies affect disease-relevant phenotypes in EGF repeat-related human disorders.

Supplementary Information: The online version contains supplementary material available at 10.1038/s41598-026-45103-1.

Keywords

Auranofin; CADASIL; Conformational change; Cysteines; Disulfide bonds; Disulfiram; NOTCH3; Split luciferase.

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