1. MAPK/ERK Pathway
  2. Mixed Lineage Kinase
  3. Necrosulfonamide

Necrosulfonamide 

Cat. No.: HY-100573 Purity: 99.23%
Handling Instructions

Necrosulfonamide is a necroptosis inhibitor acting by selectively targeting the mixed lineage kinase domain-like protein (MLKL). Necrosulfonamide prevents MLKL-RIP1-RIP3 necrosome complex from interacting with its downstream effectors. MLKL is a critical substrate of RIP3 during the induction of necrosis.

For research use only. We do not sell to patients.

Necrosulfonamide Chemical Structure

Necrosulfonamide Chemical Structure

CAS No. : 1360614-48-7

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Solution
10 mM * 1 mL in DMSO USD 79 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 79 In-stock
Estimated Time of Arrival: December 31
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5 mg USD 72 In-stock
Estimated Time of Arrival: December 31
10 mg USD 96 In-stock
Estimated Time of Arrival: December 31
25 mg USD 180 In-stock
Estimated Time of Arrival: December 31
50 mg USD 264 In-stock
Estimated Time of Arrival: December 31
100 mg USD 480 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 23 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Necrosulfonamide purchased from MCE. Usage Cited in: Transl Stroke Res. 2021 Feb 24.

    Delayed administration of Necrosulfonamide (NSA) reduces the protein level of RIP3K or MLKL and GFAP after OGD/Re injury. Astrocytes are exposed to OGD for 6 h followed by reoxygenation for 24 h. NSA (1 μM) is added to the cells upon reoxygenation.
    • Biological Activity

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    Description

    Necrosulfonamide is a necroptosis inhibitor acting by selectively targeting the mixed lineage kinase domain-like protein (MLKL). Necrosulfonamide prevents MLKL-RIP1-RIP3 necrosome complex from interacting with its downstream effectors. MLKL is a critical substrate of RIP3 during the induction of necrosis[1].

    In Vitro

    Necrosulfonamide specifically blocks necrosis downstream of RIP3 activation. Necrosulfonamide inhibits MLKL-mediated necrosis by blocking Its N-terminal CC domain function[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    461.47

    Formula

    C₁₈H₁₅N₅O₆S₂

    CAS No.
    SMILES

    O=C(NC1=CC=C(S(=O)(NC2=NC=CN=C2OC)=O)C=C1)/C=C/C3=CC=C([N+]([O-])=O)S3.[(E)]

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 28 mg/mL (60.68 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.1670 mL 10.8349 mL 21.6699 mL
    5 mM 0.4334 mL 2.1670 mL 4.3340 mL
    10 mM 0.2167 mL 1.0835 mL 2.1670 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  50% PEG300    50% saline

      Solubility: 10 mg/mL (21.67 mM); Suspended solution; Need ultrasonic

    • 2.

      Add each solvent one by one:  20% SBE-β-CD in saline

      Solubility: 6.67 mg/mL (14.45 mM); Suspended solution; Need ultrasonic

    • 3.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: 2.5 mg/mL (5.42 mM); Suspended solution; Need ultrasonic

    • 4.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: 2.5 mg/mL (5.42 mM); Suspended solution; Need ultrasonic

    • 5.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: 2.5 mg/mL (5.42 mM); Suspended solution; Need ultrasonic

    *All of the co-solvents are provided by MCE.
    References

    Purity: 99.23%

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    Keywords:

    NecrosulfonamideMixed Lineage KinaseMLKssubstrateRIP3necrosisnecrosomeformationMLKL-RIP1-RIP3complexInhibitorinhibitorinhibit

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    Product Name:
    Necrosulfonamide
    Cat. No.:
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