1. Cell Cycle/DNA Damage
    Metabolic Enzyme/Protease
  2. HSP

AT13387 (Synonyms: Onalespib)

Cat. No.: HY-14463 Purity: 99.64%
Handling Instructions

AT13387 is a potent inhibitor of Hsp90, with Kd of 0.71 nM.

For research use only. We do not sell to patients.
AT13387 Chemical Structure

AT13387 Chemical Structure

CAS No. : 912999-49-6

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 119 In-stock
5 mg USD 108 In-stock
10 mg USD 180 In-stock
50 mg USD 420 In-stock
100 mg USD 684 In-stock
200 mg   Get quote  
500 mg   Get quote  

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    AT13387 purchased from MCE. Usage Cited in: Sci Rep. 2017 Mar 15;7(1):201.

    For the caspase 3/7 assay, the HSP90 inhibitor-treated 5637 or SV-HUC cells are stained with Nexcelom ViaStain Caspase 3/7 reagent and Hoechst 33342. Caspase 3/7 positive cells are identified using the Celigo imaging cytometer, and the percentage of apoptotic caspase 3/7 positive cells is calculated with the Celigo software.
    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References


    AT13387 is a potent inhibitor of Hsp90, with Kd of 0.71 nM.

    IC50 & Target

    Kd: 0.71 nM (Hsp90)[1]

    In Vitro

    AT13387 is a potent inhibitor of Hsp90, with Kd of 0.71 nM. AT13387 shows potent antiproliferative activity in HCT116 cells, with an IC50 of 31 nM. AT13387 also strongly inhibits the proliferation of a panel of human tumor cell lines, showing IC50 of < 100 nM[1]. AT13387 exhibits cytotoxic activity against many of the PPTP cell lines, with median IC50 of 41 nM[2].

    In Vivo

    AT13387 (60 mg/kg, ip 3 days on and 3 days off for four cycles) shows antitumor activity in nude BALB/c mice bearing early stage HCT116 human colon carcinoma xenografts[1]. AT13387 (40 or 60 mg/kg, i.p.) induces significant differences in EFS distribution compared to controls in 17% evaluable solid tumor xenografts, but in none of the ALL xenografts[2].

    Clinical Trial
    Preparing Stock Solutions
    Concentration Volume Mass 1 mg 5 mg 10 mg
    1 mM 2.4419 mL 12.2094 mL 24.4188 mL
    5 mM 0.4884 mL 2.4419 mL 4.8838 mL
    10 mM 0.2442 mL 1.2209 mL 2.4419 mL
    Please refer to the solubility information to select the appropriate solvent.
    Cell Assay

    In vitro testing is performed using DIMSCAN. Cells are incubated in the presence of AT13387 for 96 hours at concentrations from 1 nM to 10 μM[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration

    AT13387 lactate is formulated in 17.5% hydroxypropyl-β-cyclodextrin.

    HCT116 cells are injected SC into the right hind flank of male nude BALB/c mice. Tumours are apparent 7 to 10 days later. Mice are arranged into matched groups of 12 according to tumour volume giving a group mean of approximately 100 mm[3] at initiation of dosing. Tumour volumes are measured every 2 days. Statistical significance between groups is assessed using nonparametric one-way ANOVA. Mice are given the lactate salt of AT13387 using a repeated cycle of dosing of once per day for three days, no dose for three days, once per day for three days etc., for four dosing cycles at 60 mg/kg/dose (as free base equivalents) dissolved in 17.5% hydroxypropyl-β-cyclodextrin via the IP route. Control mice receive dose vehicle only via the same route. Tolerability is assessed by recording body weight, clinical observations and survival[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight




    CAS No.




    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month

    Room temperature in continental US; may vary elsewhere

    Solvent & Solubility

    DMSO: ≥ 33 mg/mL

    * "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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