2. Apoptosis Anti-infection
  3. TNF Receptor Bacterial
  4. Adalimumab

アダリムマブ  (Synonyms: Adalimumab; Anti-Human TNF-alpha)

製品番号: HY-P9908 純度: 99.27%
COA Technical Support

Adalimumab is a human monoclonal IgG1 antibody targeting tumour necrosis factor α (TNF-α).

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研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 331731-18-1

容量 価格（税別） 在庫状況 数量
>無料サンプル (100 μg)   今すぐ申し込む  
1 mg $280 在庫あり
5 mg $670 在庫あり
10 mg $1150 在庫あり
25 mg $2550 在庫あり
50 mg $4080 在庫あり
100 mg   お問い合わせ  
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カスタマーレビュー

Based on 12 publication(s) in Google Scholar

Adalimumab Featured Recommendations:

アダリムマブ 関連抗体

Top Publications Citing Use of Products

    Adalimumab purchased from MedChemExpress. Usage Cited in: Adv Mater. 2025 Dec 12:e13952.  [Abstract]

    Inhibitory dose-response curves to determine the IC50 for Adalimumab (5-20 μg/mL; 72 h) on secretion of TNF-α in the established rheumatoid arthritis model.

    Adalimumab purchased from MedChemExpress. Usage Cited in: Adv Mater. 2025 Dec 12:e13952.  [Abstract]

    Analysis of the ROS levels in the synovium part and cartilage part treated with Methotrexate (MTX), Adalimumab (ADA; 5-20 μg/mL; 72 h), and the combination of MTX and ADA.

    Adalimumab purchased from MedChemExpress. Usage Cited in: Adv Mater. 2025 Dec 12:e13952.  [Abstract]

    Drug testing in the 3D coculture RA model with T and B cells. The expression of Ki 67 and TNF-α on T and B cells evaluated by flow cytometer. RA, M1 Mφ incorporated 3D model that cocultured with T and B cells. RA+MTX (2 μM), RA model treated with Methotrexate. RA+ADA (5 μg/mL), RA model treated with Adalimumab. RA+MTX+ADA, RA model treated with Methotrexate and Adalimumab.

    Adalimumab purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2025 Jan 16;135(2):e178198.  [Abstract]

    Real-time PCR of gene expression in MFS patient-specific iPSC-derived VSMCs with conditional media treatment in the presence of Adalimumab (10 μg/mL; 24 h) or IgG.

    Adalimumab purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Oct 23:e2404559.  [Abstract]

    The real-time assessment data of the affinity between TNF-α protein and Adalimumab (2.5-80 nM) detected by the FED-MSPR biosensor.

    TNF Receptor アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    TNFRSF1A/CD120a TNFRSF3/CD18 TNFRSF4 TNFRSF5/CD40 TNFRSF6/Fas/CD95 TNFRSF7/CD27 TNFRSF8/CD30 TNFRSF9/4-1BB/CD137 TNFRSF10B/DR5/CD262 TNFRSF12A/TWEAK TNFRSF16/NGF Receptor/CD271 TNFRSF18/GITR/CD357

    • 生物活性

    • プロトコル

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Adalimumab is a human monoclonal IgG1 antibody targeting tumour necrosis factor α (TNF-α).

    Isotype

    Human IgG1 kappa
    Recommend Isotype Controls
    Species Reactivity

    Human
    IC50 & Target

    TNFSF2/TNFa
    体外実験

    Treatment of HUVECs with Adalimumab for up to 12 hours shows no adverse effect on their cell viability, even with a 10-fold higher concentration of Adalimumab than used in this study[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    アダリムマブ 関連抗体
    体内実験

    The elimination half-life (t1/2) of Adalimumab is long (~20 days)[1].
    The enriched fluorescent-labelled adalimumab is observed mainly in major Oil red O-positive lesions documenting good tissue penetration of the drug into the atherosclerotic plaque, without background fluorescence of the vessel wall after vehicle control injection into hypercholesterolemic Ldlr-/- mice[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    臨床実験
    Gene ID

    7124  [NCBI]

    Accession
    Application

    ELISA, FACS, Functional assay
    Conjugated

    Unconjugated
    Reconsititution

    The product can be reconstituted/diluted with sterile PBS or saline.
    分子量

    145.43 kDa

    CAS 番号
    Appearance

    Liquid

    Color

    Colorless to light yellow

    SMILES

    [Adalimumab]
    輸送条件

    Shipping with dry ice.
    Formulation

    Please refer to the lot-specific COA for specific buffer information.
    保管条件

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Format
    • Human IgG1 kappa
    Biological Activity
    • Loaded Adalimumab on AHC2 biosensor, can bind TNF-alpha/TNFSF2 Protein, Human (177a.a, His, HY-P700291) with an affinity constant of 2.247E-10 M as determined in BLI assay.
    • Immobilized TNF-alpha/TNFSF2 Protein, Human (HY-P7058) can bind Adalimumab. The EC50 for this effect is 6.753 ng/mL.
    • Flow Cytometry analysis of THP-1 cells with Adalimumab (HY-P9908, red) with Adalimumab (anti-Cytokeratin 19) (HY-P9908). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
    純度とドキュメンテーション

    純度: 99.62%

    COA (232 KB) SDS-PAGE (81 KB) SEC-HPLC (110 KB)

    Inhibitory Antibodies User Guide (603 KB)
    参考文献
    細胞実験
    [2]

    1×104 HUVECs are plated per well of 96 well plate in EGM. After reaching sub-confluency cells are cultured in different concentration of Adalimumab (0.01-10 μg/mL), IgG isotype control (10 μg/mL) and 0.1% Triton-X as positive control in 5% CO2 at 37°C. After 12 hours, 10% Alamar blue is added to each well and kept in incubator for another 6 hours. Absorbance of oxidized alamar blue is measured at 600 nm on Infinite M200 pro plate reader. All measurements are performed in triplicate[2].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    動物実験
    [2]

    Mice[2]
    In order to analyze the vascular distribution of Adalimumab, the substance is labelled using the DyLight 549 Microscale Antibody Labeling Kit. Briefly, one vial of DyLight 549 Reagent is dissolved in 1 mg Adalimumab solution (in PBS) and purified with a resin spin column to remove excess fluor. Hypercholesterolemic Ldlr-/- mice are injected with fluorophore-labelled Adalimumab (8.0 mg/kg in 200 μL PBS i.p.) for two consecutive days and sacrificed 12 hours after last injection. Vehicle injection (200 μL PBS) is used as control.

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献
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    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Adalimumab Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Adalimumab331731-18-1Anti-Human TNF-alpha, Human Antibody ABBV-3373 AntibodyTNF ReceptorBacterialTumor Necrosis Factor ReceptorTNFRInhibitorinhibitorinhibit

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    製品名:
    Adalimumab
    製品番号:
    HY-P9908
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