Cetuximab (Synonyms: C225; IMC-C225)

Name: Cetuximab
Brand: MedChemExpress
SKU: HY-P9905
Rating: 5.0 (53 reviews)

Cat. No.: HY-P9905 Purity: 99.81%: Data Sheet
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Cetuximab (C225) is a human IgG1 monoclonal antibody that inhibits epidermal growth factor receptor (EGFR), with a K_d of 0.201 nM for EGFR by SPR. Cetuximab has potent antitumor activity.

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CAS No. : 205923-56-4

사이즈	재고	수량
무료 샘플 (100 μg)	지금 신청하기
무료 샘플 (100 μg)	지금 신청하기
1 mg	해외재고보유	Estimated Time of Arrival: December 31
5 mg	해외재고보유	Estimated Time of Arrival: December 31
10 mg	해외재고보유	Estimated Time of Arrival: December 31
25 mg	해외재고보유	Estimated Time of Arrival: December 31
50 mg	해외재고보유	Estimated Time of Arrival: December 31
100 mg	견적 받기
200 mg	견적 받기

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고객리뷰

Based on 53 publication(s) in Google Scholar

Cetuximab Featured Recommendations:

53 Publications Citing Use of MCE Cetuximab

IHC

Cell Proliferation/Viability Assay

: Cetuximab purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2024 Oct 22;134(24):e182217. [Abstract]; Top: Western blots showing protein expression of MOGAT3 in RKO, RKO EC-R, HT29, and HT29 EC-R cells. Representative blots are shown. MOGAT3KO RKO EC-R and HT29 EC-R, along with RKO EC-R-CTRL and HT29 EC-R-CTRL cell lines, were exposed to 2 μM encorafenib/4 μM cetuximab for 96 hours. Bottom: Relative OD value was assessed to determine cell viability by the CCK-8 assay (n = 3).

: Cetuximab purchased from MedChemExpress. Usage Cited in: Theranostics. 2024 Aug 26;14(14):5443-5460. [Abstract]; Cell viability by administrating various concentrations of cetuximab (Cmab) (20-160 μg/mL) and 25 μM HNK in HCT116 and LoVo cells.

: Cetuximab purchased from MedChemExpress. Usage Cited in: J Control Release. 2024 Nov:375:643-653. [Abstract]; Cell viability of RKO cells after treated by different concentrations of dabrafenib and cetuximab. The concentration gradient of dabrafenib and cetuximab varied from 0.39 to 50 μg/mL.

: Cetuximab purchased from MedChemExpress. Usage Cited in: Nat Commun. 2022 Feb 1;13(1):594. [Abstract]; Cetuximab (1.5 mg/kg; i.p.; single dose). Quantitative analysis of P-EGFR in tumor sections observed by western blot analysis

: Cetuximab purchased from MedChemExpress. Usage Cited in: Nat Commun. 2022 Feb 1;13(1):594. [Abstract]; Cetuximab (1.5 mg/kg; i.p.; single dose). Quantitative analysis of P-EGFR in tumor sections observed by IHC analysis.

: Cetuximab purchased from MedChemExpress. Usage Cited in: Theranostics. 2020 Apr 6;10(11):5107-5119. [Abstract]; The effect of cetuximab and β-elemene in combination (25 μg/mL) with other cell death inhibitors on the cell viability of KRAS mutant HCT116 and Lovo cells after the treatment for 24 h.

: Cetuximab purchased from MedChemExpress. Usage Cited in: Theranostics. 2020 Apr 6;10(11):5107-5119. [Abstract]; The expression of positive regulatory proteins for ferroptosis (HO-1 and transferrin) and the negative regulatory proteins for ferroptosis (GPX4, SLC7A11, FTH1, glutaminase, and SLC40A1) were detected by western blotting after the treatment (β-elemene 125 µg/mL, cetuximab 25 µg/mL) for 24 h.

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Biological Activity
순도&문서
References
고객리뷰

제품 설명

Cetuximab (C225) is a human IgG1 monoclonal antibody that inhibits epidermal growth factor receptor (EGFR), with a K_d of 0.201 nM for EGFR by SPR. Cetuximab has potent antitumor activity^[1].

Isotype

Human IgG1 kappa

Recommend Isotype Controls

Human IgG1 kappa, Isotype Control

Species Reactivity

Human

IC₅₀ & Target^[1]

EGFR

0.147 nM (Kd, Fixed A431 cells)

RDC Antibody

Soluble EGFR

0.201 nM (Kd)

In Vitro

Cetuximab (C225) is a monoclonal antibody that inhibits epidermal growth factor receptor (EGFR), with a K_d of 0.201 nM for soluble EGFR by SPR. Cetuximab also exhibits a K_d of 0.147 nM for EGFR in fixed A431 cells by ELISA^[1].
Cetuximab (C225; 30 nM) time-dependently inhibits the proliferation of SCC-1, SCC-11B, SCC-38, and SCC-13Y cells after treatment for 8 d. Cetuximab (30 nM) causes G₀/G₁ arrest, induces apoptosis, and reduces Rb, p27^KIP1, Bcl-2, and Bax expression in SCC-13Y cells. Cetuximab (30 nM) also enhances radiosensitivity and increases radiation-induced apoptosis in SCC-13Y cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Cetuximab (1 mg/injection) has effect on the tumour volume but the effect is more pronounced on UT-SCC-14 xenografts. In UT-SCC-14 xenografts, Cetuximab significantly reduces the expression of EGFR, pEGFR and Ki67. Cetuximab significantly decreases the expression in the MCT1 and GLUT1 cells but differences are more pronounced in UT-SCC-14 xenografts^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Gene ID

1956 [NCBI]

Accession

P00533

Application

ELISA, FACS, Functional assay

Conjugated

Unconjugated

Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.

분자량

145.54 kDa

CAS No.

205923-56-4

Appearance

Liquid

Color

Colorless to light yellow

SMILES

[Cetuximab]

Formulation

Please refer to the lot-specific COA for specific buffer information.

보관

Please store the product under the recommended conditions in the Certificate of Analysis.

Format

Human IgG1 kappa

Biological Activity

Cetuximab has a significant ADCC effect on A431 cells in a dose-dependent manner.The EC₅₀ for this effect is 43.50 and 39.11 ng/mL.

순도&문서

Purity: 99.81%

Select Batch:

Data Sheet (261 KB) SDS (251 KB)

COA (232 KB)

Inhibitory Antibodies User Guide (603 KB)

References

[1]. Goldstein NI, et al. Biological efficacy of a chimeric antibody to the epidermal growth factor receptor in a human tumor xenograft model. Clin Cancer Res. 1995 Nov;1(11):1311-8. [Content Brief]

[2]. Gustafsson H, et al. EPR Oximetry of Cetuximab-Treated Head-and-Neck Tumours in a Mouse Model. Cell Biochem Biophys. 2017 Jul 29. [Content Brief]

[3]. Huang SM, et al. Epidermal growth factor receptor blockade with C225 modulates proliferation, apoptosis, and radiosensitivity in squamous cell carcinomas of the head and neck. Cancer Res. 1999 Apr 15;59(8):1935-40. [Content Brief]

Cetuximab Related Classifications

몰농도 계산기
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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass		Concentration		Volume		Molecular Weight *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)	×	Volume _(start)	=	Concentration _(final)	×	Volume _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cetuximab205923-56-4C225 IMC-C225C 225C-225Radionuclide-Drug Conjugates (RDCs)EGFREpidermal growth factor receptorErbB-1HER1Inhibitorinhibitorinhibit

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Cetuximab (Synonyms: C225; IMC-C225)

고객리뷰

Cetuximab Featured Recommendations:

Cetuximab Related Antibodies

53 Publications Citing Use of MCE Cetuximab

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Radionuclide-Drug Conjugates (RDCs) Isoform Specific Products:

View All EGFR Isoform Specific Products:

Biological Activity

순도&문서

References

고객리뷰

Cetuximab Related Antibodies

Cetuximab Related Classifications

몰농도 계산기

농도 희석 계산기

The molarity calculator equation

The dilution calculator equation

Keywords:

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