Nivolumab (Synonyms: BMS-936558; ONO-4538; MDX-1106)

Name: Nivolumab
Brand: MedChemExpress
SKU: HY-P9903
Rating: 5.0 (22 reviews)

Art. -Nr.: HY-P9903 Reinheit: 99.63%: Data Sheet
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Nivolumab is a programmed death receptor-1 (PD-1) blocking human IgG4 antibody to treat advanced (metastatic) non-small cell lung cancer.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

CAS. Nr. : 946414-94-4

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1 mg	Auf Lager	Estimated Time of Arrival: December 31
5 mg	Auf Lager	Estimated Time of Arrival: December 31
10 mg	Auf Lager	Estimated Time of Arrival: December 31
25 mg	Auf Lager	Estimated Time of Arrival: December 31
50 mg	Auf Lager	Estimated Time of Arrival: December 31
100 mg	Auf Lager	Estimated Time of Arrival: December 31
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Based on 22 publication(s) in Google Scholar

Nivolumab Spezialitätsempfehlungen:

22 Publications Citing Use of MCE Nivolumab

Cell Proliferation/Viability Assay

In Vivo Efficacy Study

ELISA

Flow Cytometry

: Nivolumab purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2025 May 22;13(5):e011080. [Abstract]; Nivolumab (25 µg/mL). FCM analysis of effector cytokine expression, including IFN-γ, in CD8⁺T cells across different treatment groups. Histogram overlays depict cytokine expression levels, while bar graphs quantify the percentage of GZMB⁺, IFN-γ⁺, and perforin⁺CD8⁺ T cells.

: Nivolumab purchased from MedChemExpress. Usage Cited in: Adv Healthc Mater. 2025 Nov 28.; Activated Jurkat cells were treated for 2 h with anti PD-1 monoclonal antibody (Nivolumab, 10 µg/mL) and then co-cultured with cancer-on-a-bead model for an additional 24 h in the continued presence of the antibody. T cells apoptosis/necrosis was analyzed with Annexin/PI staining by flow cytometry. The displayed dot plot represents one experiment, typical of three independent experiments conducted.

: Nivolumab purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Mar 22;15(1):2567. [Abstract]; Representative proliferation assay of co-cultures using patient-derived intratumor NKs and NCI-H23 cells. NK anti-tumor activity was assessed in the presence of BETi (1 µM OTX015) and/or the anti-PD-1 inhibitor Nivolumab (15 µg/mL; 24-72 h) (n = 3 co-cultures from a single patient).

: Nivolumab purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2024 Aug 13:e177413. [Abstract]; Survival of low-grade glioma GEMM mice using Kaplan-Meier analysis. IgG: n = 20 mice (median survival [MS]: 110.5 days), anti–PD-1 (Nivolumab, 200 μg/mouse; i.p.; three times per week for 3 weeks): n = 20 mice (MS: 134.5 days), anti-TIM3: n = 21 mice (MS: 253 days). Statistics (log-rank test): control versus anti–PD-1 (Nivolumab), P = 0.64; control versus anti-TIM3, P < 0.01; anti-TIM3 versus anti–PD-1 (Nivolumab), P = 0.02.

: Nivolumab purchased from MedChemExpress. Usage Cited in: Mol Ther. 2022 Feb 2;30(2):621-631. [Abstract]; IFN-γ does not increase in the context of PD-L1^−/P146R relative to PD-L1^−/WT. Co-cultures of T cells and the GC cells with or without Nivolumab (1 ng/μL; 4 days). IFN-γ concentration was measured in the supernatant. The results showed that Nivolumab significantly enhanced IFN-γ secretion from T cells.

Spezialitätsempfehlungen

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biologische Aktivität
Reinheit & Dokumentation
Verweise
Kundenbewertung

Beschreibung

Nivolumab is a programmed death receptor-1 (PD-1) blocking human IgG4 antibody to treat advanced (metastatic) non-small cell lung cancer.

Isotype

Human IgG4 kappa

Recommend Isotype Controls

Human IgG4 (S228P) kappa, Isotype Control

Species Reactivity

Human

IC₅₀ & Target

PDCD1/PD-1/CD279

In Vitro

Nivolumab binds to CHO cells expressing PD-1 with an EC₅₀ of 1.66 nM, but does not bind to the parental CHO cell line. Nivolumab binds to PD-1 on activated T cells with an EC₅₀ of 0.64 nM. Nivolumab also inhibits the interaction between PD-1 and its ligands, PD-L1 and PD-L2, with IC₅₀ values of 2.52 and 2.59 nM, respectively. Nivolumab (1.5 ng/mL) can enhance T-cell reactivity in the presence of a T-cell receptor stimulus^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Nivolumab (10 and 50 mg/kg, i.v.) is well tolerated in cynomolgus monkeys. Serum chemistry changes are limited to a reversible 28% decrease in T3 at week 13 in females treated with 50 mg/kg. T4 and TSH levels are unchanged. In males treated with 50 mg/kg, there are no changes in T3, T4, or TSH levels. Nivolumab exposure increases in an approximately dose-proportional manner between 10 and 50 mg/kg, with no substantial sex differences noted^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Klinische Studie

Gene ID

5133 [NCBI]

Accession

Q15116

Application

ELISA, FACS, Functional assay

Conjugated

Unconjugated

Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.

Molekulargewicht

143.62 kDa

CAS. Nr.

946414-94-4

Appearance

Liquid

Color

Colorless to light yellow

SMILES

[Nivolumab]

Versand

Shipping with dry ice.

Formulation

Please refer to the lot-specific COA for specific buffer information.

Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Format

Human IgG4 kappa

Biological Activity

Immobilized hu-PD-1-His can bind Nivolumab. The EC ₅₀ for this effect is 6.195 ng/mL.
Flow cytometry analysis of PD-1 overexpression HEK293 cells labelling PD-1 with Nivolumab at 67nM (Red). Goat anti human IgG Fc(Alexa Fluor488) was used as the secondary antibody at 1/400 dilution. Isotype control - human IgG (Blue). Unlabeled control - Unlabelled cells.

Reinheit & Dokumentation

Purity: 99.63%

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Data Sheet (260 KB) SDS (251 KB)

COA (232 KB)

Inhibitory Antibodies User Guide (603 KB)

Verweise

[1]. Wang C, et al. In vitro characterization of the anti-PD-1 antibody nivolumab, BMS-936558, and in vivo toxicology in non-human primates. Cancer Immunol Res. 2014 Sep;2(9):846-56. [Content Brief]

Nivolumab Related Classifications

Molaritätsrechner
Verdünnungsrechner

Die Formel zur Berechnung von Molaritäten

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass		Concentration		Volume		Molecular Weight *
	=		×		×

The dilution calculator equation

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Diese Gleichung wird häufig abgekürzt als: C₁V₁ = C₂V₂

Concentration _(start)	×	Volume _(start)	=	Concentration _(final)	×	Volume _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Nivolumab (Synonyms: BMS-936558; ONO-4538; MDX-1106)

Kundenbewertung

Nivolumab Spezialitätsempfehlungen:

Nivolumab Related Antibodies

22 Publications Citing Use of MCE Nivolumab

Spezialitätsempfehlungen

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biologische Aktivität

Reinheit & Dokumentation

Verweise

Kundenbewertung

Nivolumab Related Antibodies

Nivolumab Related Classifications

Molaritätsrechner

Verdünnungsrechner

Die Formel zur Berechnung von Molaritäten

The dilution calculator equation

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Ihre kürzlich angesehenen Produkte:

Kundenbewertung

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