2. Metabolic Enzyme/Protease
  3. Phospholipase
  4. CAY10650

CAY10650 is a highly potent cytosolic phospholipase A2α (cPLA2α) inhibitor with an IC50 value of 12 nM. CAY10650 suppresses lipid droplets formation and PGE2 secretion.

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CAY10650

CAY10650 構造式

CAS 番号 : 1233706-88-1

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 156 在庫あり
Solution
10 mM * 1 mL in DMSO USD 156 在庫あり
Solid
5 mg $150 在庫あり
10 mg $225 在庫あり
25 mg $450 在庫あり
50 mg $630 在庫あり
100 mg $850 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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Based on 8 publication(s) in Google Scholar

Other Forms of CAY10650:

CAY10650 関連抗体

Top Publications Citing Use of Products

Phospholipase アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
Phospholipase A PLD Phospholipase C

  • 生物活性

  • 純度とドキュメンテーション

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製品説明

CAY10650 is a highly potent cytosolic phospholipase A2α (cPLA2α) inhibitor with an IC50 value of 12 nM. CAY10650 suppresses lipid droplets formation and PGE2 secretion[1][2].

体外実験

CAY10650 (12 nM; 30 min; neutrophils) inhibits the expression of the phosphorylated cPLA2-α (p-cPLA2-α) in cells[1].
CAY10650 (12 nM; 2 h; neutrophils) inhibits PGE2 release in neutrophils[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CAY10650 関連抗体

Western Blot Analysis[1]

Cell Line: Neutrophils
Concentration: 12 nM
Incubation Time: 30 minutes
Result: Inhibited cPLA2-α and inhibited the expression of the p-cPLA2-α.

Western Blot Analysis[1]

Cell Line: Neutrophils
Concentration: 12 nM
Incubation Time: 2 hours
Result: Inhibited the PGE2 secretion and inhibited PGE2 realease.
体内実験

CAY10650 (50 μg/5 μL; Inject with topical eye-drop; Chinese hamsters) relieves acanthamoeba keratitis in vivo.[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Chinese hamsters with infected with parasite-laden contact lenses[1]
Dosage: 50 μg/5 μL
Administration: Inject with topical eye-drop under the contact lens; three times a day for 6 days and topically on days 7 to 20 postinfection
Result: Reduced the severity of the keratitis and hasten the onset of resolution.
Had little mild inflammation and very few PMNs infiltration in the corneal stroma.
分子量

471.50

分子式

C28H25NO6
CAS 番号
Appearance

Solid

Color

White to off-white

SMILES

O=C(CN1C(C=CC(C(O)=O)=C2)=C2C(C(C(C)C)=O)=C1)COC(C=C3)=CC=C3OC4=CC=CC=C4
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMSO : 100 mg/mL (212.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1209 mL 10.6045 mL 21.2089 mL
5 mM 0.4242 mL 2.1209 mL 4.2418 mL
10 mM 0.2121 mL 1.0604 mL 2.1209 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (5.30 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (5.30 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: 98.0%

COA (248 KB) HNMR (293 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1209 mL 10.6045 mL 21.2089 mL 53.0223 mL
5 mM 0.4242 mL 2.1209 mL 4.2418 mL 10.6045 mL
10 mM 0.2121 mL 1.0604 mL 2.1209 mL 5.3022 mL
15 mM 0.1414 mL 0.7070 mL 1.4139 mL 3.5348 mL
20 mM 0.1060 mL 0.5302 mL 1.0604 mL 2.6511 mL
25 mM 0.0848 mL 0.4242 mL 0.8484 mL 2.1209 mL
30 mM 0.0707 mL 0.3535 mL 0.7070 mL 1.7674 mL
40 mM 0.0530 mL 0.2651 mL 0.5302 mL 1.3256 mL
50 mM 0.0424 mL 0.2121 mL 0.4242 mL 1.0604 mL
60 mM 0.0353 mL 0.1767 mL 0.3535 mL 0.8837 mL
80 mM 0.0265 mL 0.1326 mL 0.2651 mL 0.6628 mL
100 mM 0.0212 mL 0.1060 mL 0.2121 mL 0.5302 mL
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CAY10650 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CAY106501233706-88-1CAY 10650CAY-10650Phospholipaselipid dropletsPGE2neutrophilsacanthamoebakeratitismannose-induced proteincorneal epithelial cellsInhibitorinhibitorinhibit

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製品名:
CAY10650
製品番号:
HY-10801
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