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  3. Calycosin

Calycosin  (Synonyms: Cyclosin; カリコシン)

製品番号: HY-N0519 純度: 99.96%
COA 取扱説明書 Technical Support

Calycosin is a compound that can be isolated from Radix Astragali. Calycosin has strong antioxidant, anti-inflammatory and apoptosis-modulating effects. Calycosin can be used for the research of ovarian cancer and breast cancer.

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研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 20575-57-9

容量 価格(税別) 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
USD 77 在庫あり
Solution
10 mM * 1 mL in DMSO USD 77 在庫あり
Solid
5 mg $70 在庫あり
10 mg $120 在庫あり
25 mg $262 在庫あり
50 mg $420 在庫あり
100 mg $630 在庫あり
200 mg   お問い合わせ  
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カスタマーレビュー

Based on 21 publication(s) in Google Scholar

Other Forms of Calycosin:

Top Publications Citing Use of Products

    Calycosin purchased from MedChemExpress. Usage Cited in: Pharm Biol. 2022 Dec;60(1):990-996.  [Abstract]

    The cell viability was measured in HK-2 cells treated with HG and different concentrations (0, 5, 10, 20, 40 and 80 μM; 48 h) of Calycosin.

    Calycosin purchased from MedChemExpress. Usage Cited in: Pharm Biol. 2022 Dec;60(1):990-996.  [Abstract]

    The levels of LDH, MDA, GSH were measured in HK-2 cells treated with HG and different concentrations (0, 10, 20 and 40 μM) of calycosin.

    Calycosin purchased from MedChemExpress. Usage Cited in: Pharm Biol. 2022 Dec;60(1):990-996.  [Abstract]

    HK-2 cells were treated with erastin (35 μM) and/or Calycosin (40 μM). Then, the lipid ROS levels were detected.

    Calycosin purchased from MedChemExpress. Usage Cited in: Pharm Biol. 2022 Dec;60(1):990-996.  [Abstract]

    Calycosin plays a protective role in db/db mouse through the inhibition of ferroptosis. The db/db mice were randomly divided into a db/db group, a db/db plus 10 mg/kg/day Calycosin group, and a db/db plus 20 mg/kg/day Calycosin group. The db/m mice served as controls. Calycosin or saline was intraperitoneally injected into the mice for 4 weeks. HE and Masson’s Trichrome staining of kidney sections.

    Calycosin purchased from MedChemExpress. Usage Cited in: Pharm Biol. 2022 Dec;60(1):990-996.  [Abstract]

    Calycosin plays a protective role in db/db mouse through the inhibition of ferroptosis. The db/db mice were randomly divided into a db/db group, a db/db plus 10 mg/kg/day Calycosin group, and a db/db plus 20 mg/kg/day Calycosin group. The db/m mice served as controls. Calycosin or saline was intraperitoneally injected into the mice for 4 weeks. Protein levels of GPX4 and NCOA4 were detected.

    Calycosin purchased from MedChemExpress. Usage Cited in: Br J Pharmacol. 2018 May;175(9):1439-1450.  [Abstract]

    Calycosin (0-10 μM) dose dependently promoted luciferase activity in COS7 cells transfected with PPARγ and PPRE‐luciferase plasmids.

    Calycosin purchased from MedChemExpress. Usage Cited in: Br J Pharmacol. 2018 May;175(9):1439-1450.  [Abstract]

    The effect of Calycosin (0-3 μM) on Ucp1 expression in primary differentiated inguinal adipocytes was analyzed using qPCR.\
    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Calycosin is a compound that can be isolated from Radix Astragali. Calycosin has strong antioxidant, anti-inflammatory and apoptosis-modulating effects. Calycosin can be used for the research of ovarian cancer and breast cancer[1][2].

    Cellular Effect
    Cell Line Type Value Description References
    A549 IC50
    > 100 3
    Compound: 14
    Cytotoxicity against human A549 cells after 72 hrs by MTT assay
    Cytotoxicity against human A549 cells after 72 hrs by MTT assay
    [PMID: 18440233]
    A549 IC50
    >100 3
    Compound: 14
    Cytotoxicity against human A549 cells after 72 hrs by MTT assay
    Cytotoxicity against human A549 cells after 72 hrs by MTT assay
    [PMID: 18440233]
    A549 IC50
    > 100 3
    Compound: 14
    Cytotoxicity against human A549 cells after 72 hrs by MTT assay
    Cytotoxicity against human A549 cells after 72 hrs by MTT assay
    [PMID: 18440233]
    B16-BL6 IC50
    > 100 3
    Compound: 14
    Cytotoxicity against mouse B16-BL6 cells after 72 hrs by MTT assay
    Cytotoxicity against mouse B16-BL6 cells after 72 hrs by MTT assay
    [PMID: 18440233]
    B16-BL6 IC50
    >100 3
    Compound: 14
    Cytotoxicity against mouse B16-BL6 cells after 72 hrs by MTT assay
    Cytotoxicity against mouse B16-BL6 cells after 72 hrs by MTT assay
    [PMID: 18440233]
    B16-BL6 IC50
    > 100 3
    Compound: 14
    Cytotoxicity against mouse B16-BL6 cells after 72 hrs by MTT assay
    Cytotoxicity against mouse B16-BL6 cells after 72 hrs by MTT assay
    [PMID: 18440233]
    BV-2 IC50
    39.56 3
    Compound: 6
    Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction based assay
    Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction based assay
    [PMID: 28911817]
    BV-2 IC50
    39.56 3
    Compound: 6
    Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction based assay
    Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction based assay
    [PMID: 28911817]
    BV-2 IC50
    39.56 3
    Compound: 6
    Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction based assay
    Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction based assay
    [PMID: 28911817]
    HeLa IC50
    >100 3
    Compound: 14
    Cytotoxicity against human HeLa cells after 72 hrs by MTT assay
    Cytotoxicity against human HeLa cells after 72 hrs by MTT assay
    [PMID: 18440233]
    HeLa IC50
    > 100 3
    Compound: 14
    Cytotoxicity against human HeLa cells after 72 hrs by MTT assay
    Cytotoxicity against human HeLa cells after 72 hrs by MTT assay
    [PMID: 18440233]
    HT-1080 IC50
    > 100 3
    Compound: 14
    Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay
    Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay
    [PMID: 18440233]
    HepG2 IC50
    >100 3
    Compound: 7
    Anticancer activity against human HepG2 cells assessed as cell viability after 48 hrs by MTT assay
    Anticancer activity against human HepG2 cells assessed as cell viability after 48 hrs by MTT assay
    [PMID: 24974349]
    HepG2 IC50
    > 100 3
    Compound: 7
    Anticancer activity against human HepG2 cells assessed as cell viability after 48 hrs by MTT assay
    Anticancer activity against human HepG2 cells assessed as cell viability after 48 hrs by MTT assay
    [PMID: 24974349]
    HeLa IC50
    > 100 3
    Compound: 14
    Cytotoxicity against human HeLa cells after 72 hrs by MTT assay
    Cytotoxicity against human HeLa cells after 72 hrs by MTT assay
    [PMID: 18440233]
    HepG2 IC50
    > 100 3
    Compound: 7
    Anticancer activity against human HepG2 cells assessed as cell viability after 48 hrs by MTT assay
    Anticancer activity against human HepG2 cells assessed as cell viability after 48 hrs by MTT assay
    [PMID: 24974349]
    HT-1080 IC50
    >100 3
    Compound: 14
    Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay
    Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay
    [PMID: 18440233]
    HT-1080 IC50
    > 100 3
    Compound: 14
    Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay
    Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay
    [PMID: 18440233]
    Lewis lung carcinoma cell line IC50
    >100 3
    Compound: 14
    Cytotoxicity against mouse LLC cells after 72 hrs by MTT assay
    Cytotoxicity against mouse LLC cells after 72 hrs by MTT assay
    [PMID: 18440233]
    Lewis lung carcinoma cell line IC50
    > 100 3
    Compound: 14
    Cytotoxicity against mouse LLC cells after 72 hrs by MTT assay
    Cytotoxicity against mouse LLC cells after 72 hrs by MTT assay
    [PMID: 18440233]
    N9 IC50
    17.43 3
    Compound: 5
    Antineuroinflammatory activity in mouse N9 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay
    Antineuroinflammatory activity in mouse N9 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay
    [PMID: 28073678]
    N9 IC50
    17.43 3
    Compound: 5
    Antineuroinflammatory activity in mouse N9 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay
    Antineuroinflammatory activity in mouse N9 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay
    [PMID: 28073678]
    RAW264.7 IC50
    > 50 3
    Compound: 9
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess assay
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess assay
    [PMID: 25592708]
    RAW264.7 IC50
    >50 3
    Compound: 9
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess assay
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess assay
    [PMID: 25592708]
    U-937 IC50
    43 3
    Compound: 7, clycosin
    Antiproliferative activity against human U937 cells after 72 hrs by WST-8 assay
    Antiproliferative activity against human U937 cells after 72 hrs by WST-8 assay
    [PMID: 17158054]
    N9 IC50
    17.43 3
    Compound: 5
    Antineuroinflammatory activity in mouse N9 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay
    Antineuroinflammatory activity in mouse N9 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay
    [PMID: 28073678]
    U-937 IC50
    49 3
    Compound: 7, clycosin
    Antiproliferative activity against human U937 cells after 24 hrs by WST-8 assay
    Antiproliferative activity against human U937 cells after 24 hrs by WST-8 assay
    [PMID: 17158054]
    U-937 IC50
    48 3
    Compound: 7, clycosin
    Antiproliferative activity against human U937 cells after 48 hrs by WST-8 assay
    Antiproliferative activity against human U937 cells after 48 hrs by WST-8 assay
    [PMID: 17158054]
    U-937 IC50
    48 3
    Compound: 7, clycosin
    Antiproliferative activity against human U937 cells after 48 hrs by WST-8 assay
    Antiproliferative activity against human U937 cells after 48 hrs by WST-8 assay
    [PMID: 17158054]
    U-937 IC50
    49 3
    Compound: 7, clycosin
    Antiproliferative activity against human U937 cells after 24 hrs by WST-8 assay
    Antiproliferative activity against human U937 cells after 24 hrs by WST-8 assay
    [PMID: 17158054]
    PC-3 IC50
    192 3
    Compound: 5
    Cytotoxicity against human PC3 cells
    Cytotoxicity against human PC3 cells
    [PMID: 16441066]
    U-937 IC50
    43 3
    Compound: 7, clycosin
    Antiproliferative activity against human U937 cells after 72 hrs by WST-8 assay
    Antiproliferative activity against human U937 cells after 72 hrs by WST-8 assay
    [PMID: 17158054]
    RAW264.7 IC50
    > 50 3
    Compound: 9
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess assay
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess assay
    [PMID: 25592708]
    U-937 IC50
    43 3
    Compound: 7, clycosin
    Antiproliferative activity against human U937 cells after 72 hrs by WST-8 assay
    Antiproliferative activity against human U937 cells after 72 hrs by WST-8 assay
    [PMID: 17158054]
    U-937 IC50
    48 3
    Compound: 7, clycosin
    Antiproliferative activity against human U937 cells after 48 hrs by WST-8 assay
    Antiproliferative activity against human U937 cells after 48 hrs by WST-8 assay
    [PMID: 17158054]
    U-937 IC50
    49 3
    Compound: 7, clycosin
    Antiproliferative activity against human U937 cells after 24 hrs by WST-8 assay
    Antiproliferative activity against human U937 cells after 24 hrs by WST-8 assay
    [PMID: 17158054]
    Vero IC50
    159 3
    Compound: 5
    Cytotoxicity against african green monkey Vero cells
    Cytotoxicity against african green monkey Vero cells
    [PMID: 16441066]
    体外実験

    Calycosin (0-100 μM, 24-72 h) inhibits the proliferation of SKOV3 cells in a dose- and time-dependent manner[1].
    Calycosin (25-100 μM, 48 h) upregulates the Bax/Bcl-2 ratio and the expression of cleaved caspase-3 and cleaved caspase-9 in a dose-dependent manner in SKOV3 cells[1].
    Calycosin (0-16 μM, 0-240 min) stimulates rapid activation of ERK1/2 in a time- and dose-dependent manner in MCF-7 cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Proliferation Assay[2]

    Cell Line: MCF-7 cells
    Concentration: 2-32 μM
    Incubation Time: 48 h
    Result: Increased the MCF-7 cell number at the doses of 2-8 μM.
    Inhibited the proliferation of MCF-7 cells at the doses of 32 μM.
    体内実験

    Calycosin (1-4 mg/kg, i.p., daily, 20 days) inhibits the protein expression of ERα in OVX mice at the doses of 1 mg/kg[2].
    Calycosin (7.5-30 mg/kg, i.g., daily, 3 days) has a neuroprotective effect against cerebral ischemia/reperfusion injury in adult male Sprague-Dawley rats[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Adult Male Sprague-Dawley Rats[3]
    Dosage: 7.5-30 mg/kg
    Administration: i.g. daily, 3 days
    Result: Improved neurological deficit and infarct volum.
    Reduced the content of malondialdehyde (MDA), protein carbonyl and reactive oxygen species (ROS).
    Increased the activities of superoxide dismutase (SOD), catalase and glutathione peroxidase (GSH-Px) in a dose-dependent manner.
    Inhibited the expression of 4-Hydroxy-2-nonenal (4-HNE).
    分子量

    284.26

    分子式

    C16H12O5

    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C1C(C2=CC=C(OC)C(O)=C2)=COC3=CC(O)=CC=C13

    Structure Classification
    Initial Source
    輸送条件

    Room temperature in continental US; may vary elsewhere.

    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : ≥ 100 mg/mL (351.79 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.5179 mL 17.5895 mL 35.1791 mL
    5 mM 0.7036 mL 3.5179 mL 7.0358 mL
    10 mM 0.3518 mL 1.7590 mL 3.5179 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます:C1V1 = C2V2

    濃度 (開始)

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    体積 (開始)

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    体積 (終了)

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    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (8.79 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (8.79 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

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    (per animal)

    g

    Dosing volume
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    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
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    +
    %
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    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.96%

    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.5179 mL 17.5895 mL 35.1791 mL 87.9477 mL
    5 mM 0.7036 mL 3.5179 mL 7.0358 mL 17.5895 mL
    10 mM 0.3518 mL 1.7590 mL 3.5179 mL 8.7948 mL
    15 mM 0.2345 mL 1.1726 mL 2.3453 mL 5.8632 mL
    20 mM 0.1759 mL 0.8795 mL 1.7590 mL 4.3974 mL
    25 mM 0.1407 mL 0.7036 mL 1.4072 mL 3.5179 mL
    30 mM 0.1173 mL 0.5863 mL 1.1726 mL 2.9316 mL
    40 mM 0.0879 mL 0.4397 mL 0.8795 mL 2.1987 mL
    50 mM 0.0704 mL 0.3518 mL 0.7036 mL 1.7590 mL
    60 mM 0.0586 mL 0.2932 mL 0.5863 mL 1.4658 mL
    80 mM 0.0440 mL 0.2199 mL 0.4397 mL 1.0993 mL
    100 mM 0.0352 mL 0.1759 mL 0.3518 mL 0.8795 mL
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    • Molarity Calculator

    • Dilution Calculator

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます:C1V1 = C2V2

    濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
    × = ×
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    製品名:
    Calycosin
    製品番号:
    HY-N0519
    数量:
    MCE 日本正規代理店: